Mesophotic and Deep Benthic Communities: Coral Propagation Technique Development (CPT) and Habitat Assessment and Evaluation (HAE) Projects during Expedition PS-22-22 on R/V Point Sur in the Gulf of Mexico
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This dataset collection contains raw (unprocessed) data, including physical, navigational, and biological data, as well as map images, stills, and video files collected during the Mesophotic and Deep Benthic Communities (MDBC) expedition PS-22-22 aboard R/V Point Sur between 2022-05-31 and 2022-06-11. The data were collected in 2022 as a part of the Mesophotic and Deep Benthic Communities (MDBC): CPT and HAE Projects. These projects were a few of many selected by the Open Ocean Trustee Implementation Group to restore natural resources injured by the 2010 Deepwater Horizon oil spill in the Gulf of Mexico. Twenty-three remotely operated vehicle (ROV) dives were conducted at twelve sites during the cruise. ROV operations were conducted to sample and survey sites for husbandry and genetics. Live coral fragments were collected for propagation at federal labs. Coral snips were also collected for microbiology, population genetics, taxonomic barcoding, and reproduction data. CTD rosette casts were conducted daily with water sampling for husbandry and environmental DNA, also known as eDNA. Data loggers were deployed to monitor temperature and currents, and one data sensor that had been previously deployed by a separate project to monitor light and temperature parameters was recovered. Highlight videos were collected for outreach and education purposes as well. Additional related processed data products may also be included in this collection as they are submitted to the NCEI archive.
Mesophotic and Deep Benthic Communities (MDBC) eDNA metabarcoding: NF2206, R/V Nancy Foster, August 2022
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Data from environmental DNA (eDNA) samples filtered from seawater collected in the Gulf of America during a research expedition on the R/V Nancy Foster in August 2022. Samples were collected at depths throughout the water column using Niskins mounted to rosettes on Connectivity-Temperature-Depth instruments (CTDs) or Niskins mounted to remote operated vehicles (ROVs). Each eDNA sample was amplified using four primer sets: MiFish 12S rRNA marker targeting ray-finned fishes; 28S rRNA marker targeting corals and other cnidarians; and 18S rRNA and CO1 mtDNA general markers for eukaryotic plankton, protists, and other invertebrates. Sequences were then processed bioinformatically to infer amplicon sequence variants (ASVs) and assign taxonomic identifications from the WoRMs database. This data was collected as part of the Mesophotic and Deep Benthic Communities (MDBC) restoration project launched after the Deepwater Horizon oil spill. The data is formatted as per guidelines for upload to the Ocean Biodiversity Information System (OBIS, https://obis.org/).
Mesophotic and Deep Benthic Communities: Mesophotic Fish and Coral Presence, Absence, and Density Data in the Northeast Gulf of Mexico from 2018-04-18 to 2020-08-04 (NCEI Accession 0276878)
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Presence, absence and density information for fish and corals in the northeast Gulf of Mexico and the West Florida shelf. Data was collected from three different projects over a two-year period from 2018-2020. The Deepwater Horizon Natural Resource Damage Assessment, Open Ocean Trustee Implementation Groupâs Mesophotic and Deep Benthic Communities Restoration: Coral Propagation Technique Development (CPT) Project funded re-analysis of previously collected, separately funded ROV video surveys for acquisition of relevant coral and sponge presence, absence, and density data, and performed a quality assurance/quality check prior to submitting the data to NCEI. Data is formatted into two Excel spreadsheets, one with observations and one with absence data. This data package also includes representative images for each site taken from ROV video. This dataset was generated by William F. Patterson III, University of Florida (UF) during ROV surveys undertaken to monitor Lionfish trap sites and to perform a count of Red Snapper at designated areas. The corresponding video was then analyzed for corals and sponges by Joe Tarnecki at UF. The coral and sponge data in this accession were funded by the Mesophotic and Deep Benthic Communities Restoration: Coral Propagation Technique Development (CPT) project. Members of the CPT Project performed a quality check on the annotations prior to submission to NCEI.