This data release is for all native bee and plant records collected at four national parks in Maryland and Virginia, 2021 and 2022. The purpose of the study was to document the floral resources and native bees that depend on them within variously-managed park grasslands, with the goal of providing managers with information that will help them maximize pollinator habitat while concurrently meeting other management objectives. The zip file "Bee_and_Plant_Metadata_NPS2021-2022.7z" contains two metadata records: 1) "Battlefield_Bees_metadata_08.07.2024.xml" contains all native bee data, and 2. "Battlefield_Plants_metadata_08.07.2024.xml" contains all plant data.

Bee-Gap describes the ecology, life-history, and distribution of 3,925 bee species in the United States that have geographical data and verified taxonomy. The database was constructed by compiling information from a broad range of internet sources and peer-reviewed journal articles. The 10 traits included in the database are: native status (native versus exotic/introduced), state and territory presence/absence, adult habitat, nesting strategy, voltinism, sociality, host bee species (for parasitic bees only), foraging strategy (generalist vs specialist), known host plants, and tongue length.

This study generated genetic 'metabarcode' data using high-throughput sequencing to characterize pollen foraging behavior of pollinating bee species on managed field habitat within units of the National Park Service. Specimens were collected within parks of the National Capital Region from 2021-2023 and subsequently identified to species or genus. DNA was then extracted from specimens using leg samples if pollen was adherent to the corbiculae ("pollen baskets") of corbiculate bees, otherwise using whole-body samples. This data release consists of three tab-delimited files and a file of DNA sequences: 1) sample.metadata.txt includes sample identifiers and the accessions they have been assigned by the National Center for Biotechnology Information (NCBI), the authoritative repository for publicly funded genetic data in the United States. These accessions can be used individually to obtain raw sequencing data or sample information at www.ncbi.nlm.nih.gov. Alternatively, the BioProject accession PRJNA1236404 can be searched to retrieve the full set of data and sample accessions listed in the file. Entity and attribute metadata are provided for this file herein. 2) ITS2.raw.pollen.counts.txt includes the inferred taxon counts at the ITS2 locus, i.e. number of ITS2 sequences in a sample attributable to each identified taxon in each sample. 3. potential.contaminants.txt lists plant taxa that were over-represented in negative controls samples within a particular sequence run. Values for these plant taxa in these runs should either be zeroed-out or adjusted based on a statistical model to account for potential sample contamination. Censoring data based on results in negative controls is a standard practice in metabarcoding. Many samples in this study were very small and/or had no visible pollen, which increases the potential for contamination as the endogenous DNA concentration is expected to be very low in these cases. 3) reference.db.fas contains the plant reference DNA sequences used for taxonomic assignment of the pollen sample sequences.

Data included in this data set are for blue vane trap captured native bees from Logan County, Colorado starting in 2012 and ending in 2014. Data were collected the number of bees captured per date, per field, and identified to genus. Net level data contains 16,229 records.

This data release includes sampling location identification and timing data as well as plant and insect pollinator taxonomic information in Conservation Reserve Program fields. Sampling took place during July and August of 2019. Fields were located on private land managed for the U.S.Department of Agriculture Conservation Reserve Program in eastern central Iowa, U.S.A.

,Pollen data from the manuscript titled: Identity and diversity of pollens collected by two managed bee species while in blueberry fields for pollination, published in Environmental Entomology. Pollen was collected from honey bee (Apis mellifera) and bumble bee (Bombus impatiens) forager returning to the colony, while in commercial highbush blueberry fields in SW Michigan for crop pollination. Pollen was identified visually using morphological features.,Funding also provided by Project GREEEN award GR18-038. Project GREEEN, Michigan’s plant agriculture initiative housed at Michigan State University, is a cooperative effort by plant-based commodity groups and businesses in cooperation with Michigan State University AgBioResearch, Michigan State University Extension and the Michigan Department of Agriculture and Rural Development to advance Michigan’s economy through plant-based agriculture.,

,Contemporary data (2017/2018): An open area on the north side of the ESGR (GPS coordinates: 42.461808, -84.011128) was the primary site for this study as it corresponds to the location of “Evans’ Old Field”, one of the areas historically sampled for bees. The field was described by Evans as a 7.7 ha abandoned field with a mid-successional community of plants surrounded by oak-hickory woods. It is now 1.3 ha of semi-open habitat with significant encroachment of the surrounding oak-hickory woods and invasive autumn olive (Elaeagnus umbellata Thunb.). The site was visited every other week during the summers of 2017 and 2018 to sample bees. In 2017, the first sampling day was June 1 and the final sampling day was September 25. In 2018, the first sampling day was May 8 and the final day was October 3. We expanded sampling in 2018 to include a wider diversity of bees with narrower phenological periods.,During each visit we sampled bees using three methods. First, we walked to the center of the open field and randomly selected a direction to start the first 25 meter transect. Three other 25 m transects were then established based on the first one, each at a 90-degree angle from the neighboring transect for a total of 100m sampled, with each transect segment moving away from a central location. Each transect was walked for 10 minutes each, a total of 40 minutes of sampling. We used aerial insect nets to collect bees found within 1.5m of the transect, and time was stopped for specimen processing. The host plant was recorded for all specimens captured from flowers. Flowering plants were identified to the lowest taxonomic level in the field using Newcomb’s guide and the PlantNet app, usually to species. Second, we spent 20 minutes collecting bees from plants of any species in the general vicinity of the open field. Third, to most closely match the methods used by Evans (see below), we spent 30 minutes sampling bees at each of the primary blooming plant species located in the field. Total time spent conducting this final sampling method varied based on the number of primary blooming plants at each visit, with a minimum of 30-minutes if there was only one primary plant. This sampling method was always done last, and included any plants that we collected more than one bee from that day. All bees were identified to species (or lowest possible taxonomic level) using relevant keys. All specimens collected in 2017 and 2018 are currently held in the Isaacs Lab at Michigan State University (as of 2024), and will eventually be deposited at the A.J. Cook Arthropod Collection at Michigan State University for long-term inclusion in that collection.,Historical data (1921-1999): The University of Michigan Museum of Zoology Insect Collection (UMMZI), Ann Arbor, MI, holds over 4,000 bee specimens from the historical collections at the ESGR, and specimens were databased as part of this study. Historical data were checked for entry errors and outdated taxonomies. Specimens with questionable species determinations were re-examined and re-identified using relevant keys (see above) where possible. Bees that could not be confidently identified to the species level were excluded from the dataset, and entries that were missing the date of collection were also removed. Excluded entries accounted for less than 1% of the specimens. There were notable gaps in records at the ESGR, as there were no focused survey efforts since Evans’ last efforts in 1989, and only occasional specimen records from 1990-1999. There were no surveys and no records for the ESGR after 1999 and prior to this study in 2017/2018. All specimens from the ESGR were included in this dataset, not only those specifically collected at the Evans’ Old Field.,In addition to the 4,000 plus records from the ESGR since 1921, we also include Evans’ dataset from his 1972 and 1973 collection effort. Evans’ original dataset from 1972/1973 was available through UM records. The dataset is unique compared to the

These raw survey data and associated explanatory covariates were used to model western bumble bee occupancy across the western conterminous United States from 1998 to 2020. These data underlie the analyses found in the publication of “Recent and future declines of a historically widespread pollinator linked to climate, land cover, and pesticides” by Janousek et al. 2023 in the Proceedings of the National Academy of Sciences vol. 120, issue 5. The covariate data are provided in a scaled format for use in the occupancy model, and the scaling parameters used to transform the data to the original (real) scale are provided in the scaling information file.