The toxicity of sediments in Pensacola, Choctawhatchee, St. Andrew and Apalachicola Bays was determined as part of bioeffects assessments performed by NOAA's National Status and Trends Program. The objectives of the survey were to determine: (1) the spatial patterns in toxicity throughout each bay, (2) the spatial extent of toxicity throughout and among the bays, (3) the severity or degree of toxicity, and (4) the relationships between chemical contamination and toxicity. The survey was conducted over two years: Pensacola Bay and St. Andrew Bay were sampled in 1993; and Choctawhatchee Bay, Apalachicola Bay and Bayou Chico (a sub-basin of Pensacola Bay) were sampled during 1994. Surficial sediment samples were collected from 123 randomly-chosen locations throughout the five areas. Multiple toxicity tests were conducted on all samples, and chemical analyses were performed on 102 of the 123 samples. Toxicological tests were conducted to determine survival, reproductive success, morphological development, metabolic activity, and genotoxicity; all bays showed toxicity in at least some of the samples. Toxicity was most severe in Bayou Chico, an industrialized basin adjoining Pensacola Bay. Other developed bayous adjoining Pensacola Bay and the other bays also showed relatively severe toxicity. The main basins of the bays generally showed lower toxicity than the adjoining bayous. The different toxicity tests, however, indicated differences in severity, incidence, spatial patterns, and spatial extent in toxicity. The most sensitive test, a bioassay of metabolic activity of bioluminescent bacteria, indicated toxicity was pervasive throughout the entire study area. The least sensitive test, an acute bioassay performed with a benthic amphipod, indicated toxicity was restricted to a very small portion of the area. Causes of toxicity were not determined in the survey. However, mixtures of potentially toxic substances, including pesticides, petroleum constituents, trace metals, and ammonia, were associated statistically with the measures of toxicity. The concentrations of many substances were highest in Bayou Chico, where the most severe toxicity was observed. At these toxic sites, some of the substances had considerably elevated concentrations, often exceeding numerical guidelines or known toxicity thresholds. The relationships between toxicity and chemical concentrations differed among the bays and toxicity tests.

Phytoplankton species abundance (cell/L) and oceanographic conditions (temperature, salinity, chlorophylle-a (mg/m³) for some years and nutrient content (mmol/m³)) at stations of the Harmful Algae Monitoring Programme (HAMP) from1994 to 2016. The layer presents the station positions of the HAMP. Two files are attached to each station: one containing the cell counts and the second the oceanographic conditions. Purpose The summer growth of many toxic and harmful microalgae species poses a serious threat for the public health and commercial or recreational exploitation of some marine species. The Department of Fisheries and Oceans (DFO) initiated the Harmful Algae Monitoring Programme (HAMP) in 1989 in order to complete the monitoring program for paralytic shellfish poisoning (PSP). Under the responsibility of Maurice-Lamontagne Institute scientists, the HAMP is to monitor, by means of a coastal station network, the natural occurrence of toxic and harmful algae in the St. Lawrence in order to determine their spatio-temporal distribution and the environmental conditions leading to their bloom. The network is made up of 11 coastal stations which are sampled every week from April to November and which are established along Quebec eastern shores. It extends from Tadoussac to Tête-à-la-Baleine on the St. Lawrence north shore and from Sainte-Flavie to Carleton on the south shore along the Gaspé peninsula. Another station is located in Havre-Aux-Maisons, Magdalen Islands. The HAMP was discontinued in 2010 but opportunistic samplings are still done at some stations. Additional information The sampling and analysis protocol is described in details in the following publication apart from the fact that the number of identified and counted species significantly has been increasing with time. Phytoplankton samples are preserved in a lugol solution. Blasco D., M. Levasseur, R. Gélinas, R. Larocque, A.D. Cembella, B. Huppertz et E. Bonneau.1998. Monitorage du phytoplancton toxique et des toxines de type IPM dans les mollusques du Saint-Laurent: 1989 à 1994. Rapp. stat. can. hydrogr. sci. océan. 15 1 : x i-117 p.

The U.S. Environmental Protection Agency (EPA) National Coastal Condition Assessment (NCCA) is a nationwide survey of coastal and estuarine water quality. During the 2015 EPA NCCA, samples were collected at 732 sites for analysis of algal toxins and cyanotoxins at the Organic Geochemistry Research Laboratory (OGRL) at the U.S. Geological Survey Kansas Water Science Center (KSWSC) by liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS). Samples from the Atlantic, Gulf, and Pacific coasts of the conterminous U.S. were analyzed for anatoxin-a, cylindrospermopsin, domoic acid, dinophysistoxin-1, dinophysistoxin-2, gymnodimine, 10 microcystin congeners, nodularin, okadaic acid, pectenotoxin-2, and 13-desmethyl spirolide c.

Harmful algal blooms of the toxic dinoflagellate, Karenia brevis, have caused massive fish kills in the Gulf of Mexico since the 1500's, with most occurrences on the west coast of Florida. In 1996, the list of states that have experiences natural resource, public health and economic impacts related to this organism expanded, with the addition of Alabama, Mississippi, and Louisiana, to include all the Gulf-coast states and North Carolina. Estimates of economic impacts to Florida and North Carolina from two moderate intensity blooms ranged from 15 to 25 million dollars respectively. The harmful impacts caused by K. brevis occur only when cell concentrations increase significantly above low background concentrations that are present year-round in the Eastern Gulf of Mexico. Once a bloom has developed offshore in typically oligotrophic waters, cell concentrations at the 105 level can be maintained for months. During 21 of the past 22 years, red tide blooms have been observed within the region between Tampa Bay and Charlotte Harbor. The key to understanding any HAB lies in knowing how one algal species has adapted and come to dominate in its particular realm of physical, biological and chemical conditions. Our ability to predict initiation, maintenance, and dispersal of blooms on the Florida shelf has been severely limited by the lack of a quantitative description, or model, of their population dynamics and the physical, biological and chemical regime in which they are embedded. The modeling components of this project will incorporate the quantitative description of blooms and their surrounding environment provided by the field and laboratory portions of this project. The field component will employ a set of annual process cruises.

An Ecopath with Ecosim (EwE) model of the WFS explicitly simulating HABs was recently developed for illuminating the potential ecosystem impacts of red tides within the WFS region. These results were produced by research funded by the National Oceanic and Atmospheric Administration (NOAA’s) RESTORE Act Science Program under award NA15NOS4510233 a the University of Miami and award NA15NOS4510232 to the University of South Florida.

The EPA National Coastal Condition Assessment (NCCA) is a nation-wide survey of coastal and estuarine water quality. During the 2015 EPA NCCA, samples were collected for analysis of algal toxins and cyanotoxins at the Organic Geochemistry Research Laboratory (OGRL) at the U.S. Geological Survey Kansas Water Science Center (KSWSC) by liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS). The 542 samples collected from the Great Lakes were analyzed for anatoxin-a, cylindrospermopsin, domoic acid, 10 microcystin congeners, nodularin, and okadaic acid. A subset of samples were also analyzed for dinophysistoxin-1, dinophysistoxin-2, gymnodimine, pectenotoxin-2, and 13-desmethyl spirolide c.

The database provides information on prescribed amounts, levels detected in aquatic environments, chemical structure, molecular weight, octanol-water partition coefficients, water solubility, environmental persistence, general toxicity information and specific toxicity levels to five groups of organisms (algae, mollusks, finfish, crustaceans, and select terrestrial animals).

This study was based on the sediment quality triad (SQT) approach. A stratified probabilistic sampling design was utilized to characterize the Kachemak Bay system in terms of chemical contamination, sediment toxicity and benthic infaunal community structure. The purpose was to define the extent and magnitude of toxicity and other biological effects associated with contaminants in the Kachemak Bay system. Five strata (Homer harbor, Western intertidal, Western subtidal, Eastern intertidal, and Eastern subtidal) were established in the shallow (less than 10 fathoms) northern area of the bay. Sediment samples were collected at multiple stations in each strata. A broad suite of sediment contaminants were analyzed at each station, including polynuclear aromatic hydrocarbons (PAHs), chlorinated pesticides including DDT and its metabolites, polychlorinated biphenyls (PCBs), trace elements, and butyl-tins Other parameters included grain size analysis, total organic/inorganic carbon (TOC/TIC), and percent solids. Characterization of infaunal assemblages and the abundance of organisms present in sediments provide additional information to help determine areas of degraded sediments. Whole sediment toxicity bioassays with two species of amphipod were conducted to test for overt contaminant toxicity. This project provides invaluable baseline data on sediment infauna species richness, chemical contamination and toxicity that is georeferenced and posted on the internet through the NOAA's National Status and Trends data portal.