It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from infected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered saline as an untreated control.

It is unclear whether DNA (Deoxyribonucleic Acid) lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data release consists of three tabular datasets of raw absorbance measurements that were collected with a BioRad Model 3550 ELISA (Enzyme linked Immunosorbant Assay) plate reader at a wavelength setting of 405 nm from serial dilutions of whole blood that were preserved with either PBS (phosphate buffered saline) or two different DNA lysis buffers and a single tabular dataset of Percent ELISA Values that were derived from the raw absorbance measurements. The whole blood was collected from uninfected Hawaii Amakihi and Hawaii Amakihi experimentally infected with Plasmodium relictum that were collected from Upper Waikea Forest Reserve on Hawaii Island.

It is unclear whether DNA (Deoxyribonucleic Acid) lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data release consists of three tabular datasets of raw absorbance measurements that were collected with a BioRad Model 3550 ELISA (Enzyme linked Immunosorbant Assay) plate reader at a wavelength setting of 405 nm from serial dilutions of whole blood that were preserved with either PBS (phosphate buffered saline) or two different DNA lysis buffers and a single tabular dataset of Percent ELISA Values that were derived from the raw absorbance measurements. The whole blood was collected from uninfected Hawaii Amakihi and Hawaii Amakihi experimentally infected with Plasmodium relictum that were collected from Upper Waikea Forest Reserve on Hawaii Island.

It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides derived Percent ELISA (Enzyme linked Immunosorbant Assay) Values calculated from adjusted absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm.

It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set reports raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an infected Hawaii Amakihi that were preserved in PBS (phosphate buffered saline), TEN (Tris-Ethylenediaminetetraacetic Acid) Buffer, or TEN buffer that was subsequently treated with SDS (sodium dodecyl sulfate - ethylenediaminetetracetic acid) and Proteinase K.

It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set reports raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an infected Hawaii Amakihi that were preserved in PBS (phosphate buffered saline), TEN (Tris-Ethylenediaminetetraacetic Acid) Buffer, or TEN buffer that was subsequently treated with SDS (sodium dodecyl sulfate - ethylenediaminetetracetic acid) and Proteinase K.

Native and introduced forest birds were captured and then released across the Hawaiian Islands to acquire a blood sample for obtaining DNA and test for exposure to avian malaria (Plasmodium relictum). A total of 2,945 samples were collected and analyzed for avian malaria prevalence from 39 species captured at 66 sites from Kauai, Oahu, Molokai, Maui, and Hawaii islands.

Native and introduced forest birds were captured and then released across the Hawaiian Islands to acquire a blood sample for obtaining DNA and test for exposure to avian malaria (Plasmodium relictum). A total of 2,945 samples were collected and analyzed for avian malaria prevalence from 39 species captured at 66 sites from Kauai, Oahu, Molokai, Maui, and Hawaii islands.

This data publication contains information collected as part of a field and laboratory effort to determine prevalence of avian malaria (Plasmodium relictum) and pox-like lesions (Avipoxvirus spp.) in native and non-native forest birds on the northern, high elevation (>1500 m) slopes of Haleakala volcano on Maui during the last decade of the 20th century. Forest bird banding data and blood samples for diagnosis of Plasmodium relictum, the agent responsible for avian malaria in Hawaii, were collected in 1993 and again in 1996 at Hanawi Natural Area Reserve and Waikamoi Preserve. Both areas comprise critical habitat for several species of endangered forest birds including Akohekohe (Palmeria dolei), Kiwikiu (Pseudonestor xanthophrys) and Poouli (Melamprosops phaeosoma) which were still extant in the wild at the time of the study. This data file reports morphometric data, age, sex, molt status, lesion occurrence, and diagnostic results for avian malaria from native and non-native forest birds that were captured with mist nets at the two study sites. This XML describes one tabular CSV file: Maui Banding Diagnostics Data Final.CSV.

This data publication contains information collected as part of a field, laboratory, and modeling effort aimed at uncovering ecological drivers of avian malaria transmission and impacts on Hawaiian honeycreepers across an altitudinal gradient on the eastern flank of Mauna Loa and Kilauea volcanoes. Forest bird banding data and blood samples for diagnosis of Plasmodium relictum, the agent responsible for avian malaria in Hawaii, were collected from 2001 - 2005 at nine sites ranging 25 to 1,800 m above sea level. This data file reports morphometric data, age, sex, molt status and diagnostic results for avian malaria from native and non-native forest birds that were captured with mist nets at the nine study sites. Related data describing site and sampling coordinate data, mosquito capture data, mosquito avian malaria infection data, and point-transect distance sampling data is available at https://doi.org/10.5066/P95LVJIC. This XML describes one tabular CSV file: Banding Diagnostics Merge Final.CSV.