This dataset contains sample information about the origin of the Desmognathus wrighti and Plethodon jordani salamanders collected from Great Smoky Mountains National Park and sequenced, and their corresponding SNP genotype data. These data were used to examine patterns of genomic structure of these two salamander species within Great Smoky Mountains National Park. The raw sequence data are archived in the GenBank Bioproject PRJNA1062342 at: https://www.ncbi.nlm.nih.gov/bioproject/

Summary of proposed field methods and activities: Sampling Myriapods is a low-impact process. Collecting is done by turning over leaf piles with a millipede rake (see Means et al. 2015) to expose specimens at the soil-leaf interface. Rocks and logs are also rolled over to search for specimens. Leaf litter samples are sometimes taken and processed with a Berlese funnel to extract small-bodied individuals. After an area has been searched, logs and rocks are turned back over and leaves spread out to return the area to its pre-disturbed state. Numbers of Myriapods collected will range from 10-30 specimens, depending on quality of the habitat and success of the search. Both millipedes (Diplopoda) and centipedes (Chilopoda) will be collected, to contribute to related research on species groups by the Marek Lab. In the case of leaf litter samples, other incidental leaf litter arthropods including insects and spiders are collected as well, but not in numbers large enough to cause strains on the local populations.

Summary of proposed field methods and activities: Sampling Myriapods is a low-impact process. Collecting is done by turning over leaf piles with a millipede rake (see Means et al. 2015) to expose specimens at the soil-leaf interface. Rocks and logs are also rolled over to search for specimens. Leaf litter samples are sometimes taken and processed with a Berlese funnel to extract small-bodied individuals. After an area has been searched, logs and rocks are turned back over and leaves spread out to return the area to its pre-disturbed state. Numbers of Myriapods collected will range from 10-30 specimens, depending on quality of the habitat and success of the search. Both millipedes (Diplopoda) and centipedes (Chilopoda) will be collected, to contribute to related research on species groups by the Marek Lab. In the case of leaf litter samples, other incidental leaf litter arthropods including insects and spiders are collected as well, but not in numbers large enough to cause strains on the local populations.

Field Methods: Fly collections. Drosophila will be collected from forests during summer months (May to October) by sweep-netting or aspirating over naturally-occurring mushrooms. In some instances, boiled and thus killed commercial mushrooms (Agaricus bisporus) will be set out as baits. In addition, flies may be bred from field-collected mushrooms. Laboratory maintenance of fly cultures. In the laboratory, the culture medium consists of Instant Drosophila medium (Carolina Biological), a small piece of commercial mushroom, and a dental cotton roll, which serves as a pupation site. Cultures are maintained at 20C, 60%RH, and 14:10 light:dark schedule.

Field Methods: Fly collections. Drosophila will be collected from forests during summer months (May to October) by sweep-netting or aspirating over naturally-occurring mushrooms. In some instances, boiled and thus killed commercial mushrooms (Agaricus bisporus) will be set out as baits. In addition, flies may be bred from field-collected mushrooms. Laboratory maintenance of fly cultures. In the laboratory, the culture medium consists of Instant Drosophila medium (Carolina Biological), a small piece of commercial mushroom, and a dental cotton roll, which serves as a pupation site. Cultures are maintained at 20C, 60%RH, and 14:10 light:dark schedule.

This data set contains individual museum catalog numbers, associated Genbank accession numbers, and species designations based on the mitochondrial genome and ten nuclear loci for 55 Martes americana, 28 Martes caurina, 2 Martes of uncertain taxonomic designation based on genetic data alone, and one each of Martes zibellina, M. pennanti, Gulo gulo, Neovison vison, and Mustela frenata.

Field Methods: Collection of 10-20 samples of about 20 g of soil from just under the leaf litter of assorted forest locations. Soil is scraped only from the surface of patches about 10 cm square and stored in small sterile plastic collection bags. No digging is necessary because the organisms are only on the surface. The soil will be cultured in the laboratory to produce spores and then cloned and stored for future research. Dictyostelium is a common model organism and we are developing a library of wild strains to compare.

This data release comprises a dataset that contains sample collection information and microsatellite genotypes, and another dataset that contains single nucleotide polymorphism (SNP) genotypes with sample collection information for populations of white-tailed ptarmigan across the species' range. There is also an additional file (accession numbers.xlsx) linking samples to accession numbers in Genbank. This data was collected in order to address the following: The delineation of intraspecific units that are evolutionarily and demographically distinct is an important step in the development of species-specific management plans. Neutral genetic variation has served as the primary data source for delineating units for conservation, but with recent advances in genomic technology, we now have an unprecedented ability to utilize information about neutral and adaptive variation across the entire genome. Here, we use traditional genetic markers (microsatellites) and a newer reduced-representation genomic approach (single nucleotide polymorphisms) to delineate distinct groups of white-tailed ptarmigan (Lagopus leucura), an alpine-obligate species that is distributed in naturally fragmented habitats from Alaska to New Mexico.

This data release comprises a dataset that contains sample collection information and microsatellite genotypes, and another dataset that contains single nucleotide polymorphism (SNP) genotypes with sample collection information for populations of white-tailed ptarmigan across the species' range. There is also an additional file (accession numbers.xlsx) linking samples to accession numbers in Genbank. This data was collected in order to address the following: The delineation of intraspecific units that are evolutionarily and demographically distinct is an important step in the development of species-specific management plans. Neutral genetic variation has served as the primary data source for delineating units for conservation, but with recent advances in genomic technology, we now have an unprecedented ability to utilize information about neutral and adaptive variation across the entire genome. Here, we use traditional genetic markers (microsatellites) and a newer reduced-representation genomic approach (single nucleotide polymorphisms) to delineate distinct groups of white-tailed ptarmigan (Lagopus leucura), an alpine-obligate species that is distributed in naturally fragmented habitats from Alaska to New Mexico.

These data were compiled to provide land-management relevant information on the native and nonnative subspecies (subsp.) of Phragmites australis (subsp. americanus and subsp. australis, respectively) in Glen Canyon National Recreation Area (NRA), Grand Canyon National Park (NP), Arches National Park, and Bill Williams National Wildlife Refuge. Specifically, the goals of this work were to determine the extent and distribution of Phragmites australis subspecies in the Glen and Grand Canyon regions, evaluate if P.a. subsp. americanus can be reliably distinguished from P.a. subsp. australis using morphological characters in this region, and determine if P.a. subsp. americanus exhibits genetic structure in the study area. The objectives of our study were to determine how prevalent the nonnative Phragmites is in the study area and support native plant material development for restoration activities in Glen Canyon NRA and Grand Canyon NP. These data represent real-time PCR cycle threshold values (Ct scores) for two target regions, nuclear microsatellite data for seven loci, and morphological data. These data were collected for 84 sites including five nonnative Phragmites locations and 79 native Phragmites locations. Tissue samples were mostly collected along the Colorado River between Glen Canyon Dam and Lake Mead, around Lake Powell, and in tributaries to the Colorado River from 2021 through 2024. Tissue samples and herbarium collections were collected from one to fifteen ramets across each stand, where multiple samples from one stand were spread across its full area. Reference samples for both the native and nonnative Phragmites, Phragmites australis subsp. berlandieri, and a hybrid of P.a. australis and P.a. americanus were acquired and included in analyses. Samples were dried and total genomic DNA was extracted using Qiagen DNeasy Plant MiniKits. Real-time polymerase chain reactions (PCR) with two target regions, AMER and AMAU, were used to identify nonnative Phragmites individuals (Lindsay et al, 2023). We then amplified 7 microsatellite loci (Saltonstall, 2003; Meyerson and others, 2010) using PCR and analyzed the fragments on an ABI 3730XL Genetic Analyzer with GeneScan LIZ500 internal size standard. Although P. australis is polyploid, these loci all had no more than two alleles, so were treated as diploid data. We then collected field and lab data on morphological characters for the stands we genetically tested, following previously described diagnostic characters (Swearington and Saltonstall, 2012; McTavish et al, 2023) These data can be used to evaluate if a stand of Phragmites is nonnative, native, or a hybrid of the two. It can also be used to determine genetic diversity and structure across the sampled stands. Finally, they can be used to assess morphological variability in native Phragmites stands across the region.