The goal of the tidal-fluvial estuary study is to determine the estuary's contribution to the spatial structure and life history diversity of Columbia River salmon stocks and the implications for estuary restoration. The study targets salmon use of tidal-fresh habitats in the estuary from Rkm 75 to Bonneville Dam, and addresses four primary objectives: 1. Characterize the temporal and spatial distribution of Chinook salmon genetic stock groups throughout the estuary (March 2010 - March 2012). 2. Determine stock-specific habitat use, life histories, and performance of juvenile salmon in key habitat complexes to fill data gaps in the tidal fluvial reaches of the estuary (2012-2016). 3. Monitor juvenile salmon life histories and their contributions to adult returns in selected estuary tributaries, including tributary examples where tidal habitats have been restored (2012-2018). 4. Evaluate estuary restoration needs for recovery of all salmon ESUs and account for projected effects of climate change through application of a salmon life-cycle model (2011-1015). The study, funded by the U.S. Army Corps of Engineers, involves a large team of researchers organized by NOAA Fisheries, including researchers from the Oregon Health and Sciences University, University of Washington, and Washington Department of Fish and Wildlife. The study addresses critical uncertainties identified in the research, monitoring, and evaluation (RME) program for the Federal Columbia River Estuary Program (FCREP). The Estuary Program is intended to conserve and restore the estuary ecosystem to improve the performance of listed salmonid populations. Products from the tidal-fluvial study will include: 1. Descriptions of stock-specific temporal and spatial distributions of Chinook salmon throughout the estuary. 2. Estimates of variations in Chinook salmon stock composition and stock-specific growth, food habits, consumption rates, and bioenergetic efficiencies within selected tidal-fluvial habitats. 3. Estimated contributions of estuarine life histories among returning adult Chinook salmon from selected populations throughout the Columbia River Basin. 4. A hydrological model quantifying the dynamics of rearing habitat opportunities for juvenile salmon at estuary reach and habitat scales. 5. Improved life-cycle models to account for the estuarine life histories of juvenile salmon and estimating the potential effectiveness of estuary restoration actions on the recovery and viability of selected salmon stocks. These results will directly address information needs to support estuary actions specified in the Federal Columbia River Power System (FCRPS) Biological Opinion for the Columbia River. The tidal-fluvial estuary study is part of an ongoing estuary research program initiated in 2002. The current study expands upon earlier research conducted in the lower 100 km of the estuary from 2002 to 2008. Although all objectives will be addressed by 2018 to correspond with a review of progress implementing the FCRPS Biological Opinion, some sampling activities may extend beyond this date to allow brood-year reconstruction of estuary contributions to adult returns in selected streams (Objective 3). Bimonthly genetic stock group distribution for juvenile Chinook Salmon collected from 3 habitats each from 6 tidal-fluvial estuary reaches and monthly fish species composition, abundance, and length:weight; Chinook salmon life history and genetic stock ID.

This is a database of a variety of biological, reproductive, and energetic data collected from fish on the continental shelf in the northwest Atlantic Ocean. Species sampled in this database thus far include winter flounder, yellowtail flounder, summer flounder, haddock, cusk, Atlantic wolffish, and Atlantic herring. Data are collected from fish provided principally from fishermen participating in the NECRP Study Fleet. Some fish are taken from other NECRP research studies, and a small number from NEFSC surveys and surveys by MADMF and URI GSO. The catch location data is provided in views from the relevant FVTR, SVDBS, or other tables for a few cooperative research or external programs. The biological data includes general physical data (weights, lengths, organ weights, macroscopic maturity stage), age data, and other reproductive data. Measures collected from preserved gonad samples includes data for estimation of fish fecundity (oocyte counts and diameters) and from grading gonad histology for determination of maturity and seasonal reproductive status. In addition, relative measures of energetic condition are collected (including tissue wet weight and dry weights and bioimpedance data), and for some fish food habits data were collected in the first 18 months of data collection.

Sablefish (Anoplopoma fimbria) have a wide distribution along the Pacific coast, extending from Baja California to Alaska, the Bering Sea and through to the eastern coast of Japan. A unique feature of these fish is the wide variation in temperature and depth that sablefish experience throughout their life cycle, extending from depths 200m as adults to the surface as larvae and juveniles. While the landed weight of sablefish in the commercial fishery is relatively small, the exceptionally high value of this species ranks it 3rd in economic value to walleye pollock and Pacific cod. As such, sablefish are highly managed throughout the Pacific, and understanding the biology of this species is essential for proper management. The aim of this project is to characterize the reproductive life history of two populations of sablefish in coastal Washington and California. Fish will be collected from the same geographical location on a monthly basis for one year. The reproductive status will be determined from gonadal histology and plasma sex steroid levels, and age will be determined from otoliths. It is expected that data on size, age, rate of gonadal development, seasonal timing of spawning, fecundity, frequency of reproduction, and potential shifts in distribution of sexes will be obtained. This study applies directly to the Magnuson-Stevens Fishery Conservation and Management Reauthorization Act, because the data will be used to improve stock assessments and estimates of spawning biomass in this commercially important species. This project is a cooperation with the commercial fishing industry and scientists at the Northwest Fisheries Science Center (NWFSC) and Southwest Fisheries Science Center (SWFSC). Size, sex, gonad stage, fecundity.

The southern residents face several well-documented external threats. However, the population might also be subject to internal factors that limit population growth, including a reduction in fitness due to inbreeding. Understanding how inbreeding affects individual fitness and thus the health status of the population is critical for evaluating the relative influence of other factors on southern resident recovery. Assessing the risk of inbreeding depression – specifically called for in the NMFS recovery plan – is important for conducting accurate Population Viability Analyses and correctly understanding the urgency of recovery efforts. Here, we propose using genomic methods to evaluate inbreeding and inbreeding depression in the southern resident population and a comparable but healthier Alaskan resident population. Measures of inbreeding will serve as an important health marker, supporting the integration of individual metrics aimed at understanding population performance. Measures of inbreeding can be obtained directly by estimating variation at millions of DNA markers in an individual’s genome. Complete genomic sequences for 100 southern and 50 Alaska residents will be collected in collaboration with the genomics company BGI. Inbreeding values for each individual will be obtained using genome wide measures of homozygosity and relatedness. We will then combine measured of inbreeding with data on individual fitness, to evaluate whether inbreeding leads to inbreeding depression. Generalized additive models will be used to determine whether survivorship, fecundity and size-at-age is influenced by different levels of inbreeding. Using this data, we will measure the degree of current and predicted future of inbreeding in the southern residents and compare this risk with the Alaska residents that have experienced consistent population growth. We will then evaluate whether inbreeding depression explains individual variance in fitness, and estimate its influence on the status of southern residents, using Population Viability Analyses. Microsatellite, SNP, and mtDNA sequence data from southern resident killer whales.

Samples of skin and other tissues are collected from marine mammals along the North-Central Gulf of Mexico from live animals through remote biopsy or live-capture or stranded animals during 2010-2014. DNA is extracted from these samples, sequenced, and analyzed using a variety of methods to support phylogenetic studies, stock delineation studies, and a variety of other analyses related to cetacean genetics associated with the Natural Resources Damage Assessment associated with the Deepwater Horizon oil spill. These data sets include the sequence and other genetic data collected from these samples along with analytical results. These data support stock delineation studies and a variety of other analyses related to cetacean genetics and are incorporated into the Deepwater Horizon Damage Assessment and Restoration Plan.

Data collected on this cruise included the following: We conducted a juvenile fish and benthic fish prey survery in the eastern Bering Sea (61 3-meter beam trawls, 64 modified tucker epibenthic plankton sled tows, 54 van veen benthic grab collections).

This project evaluates data from coded wire tagging with that from parental based tagging to identify stock of origin for Chinook salmon landed in Washington state commercial troll fishery.