This repository holds genome assemblies for Staphylococcus, Burkholderia, and Ralstonia bacterial isolates isolated from the ISS between the years of 2006-2015. Information on the overall microbial isolation effort is stored under NASA's Life Science Data Archive experiment 13823 (https://lsda.jsc.nasa.gov/Experiment/exper/13823). For the genomes presented here, isolates were received from NASA's Johnson Space Center and their genomes were sequenced and assembled thanks to NASA Space Biology awards to Michael D. Lee (NNH16ZTT001N-MOBE) and Aubrie O'Rourke (80NSSC17K0035).

As part of an ongoing effort to catalogue microbial communities inhabiting the International Space Station (ISS) crew-associated environmental samples were collected from the Japanese Kibo Russian and US research modules. Initial analysis based on 16S rRNA gene sequencing identified 11 Bacillus isolates (two from Kibo Japanese Experiment Module (JEM) four from US Segment Harmony Node 2 and five from Russian Segment Zvezda module sites) all belonging to the Bacillus anthracis-B. cereus-B. thuringiensis group. Isolates were further characterized by whole genome comparative analysis. Each isolate was sequenced assembled and aligned against all members of the B. cereus sensu lato group. Based on genome size estimates (5.2 - 5.3 Mbp) strain nucleotide identity (>99.99%) and maximum likelihood phylogenetic placement all isolates were found to exhibit a very high level of similarity. With respect to gene content all isolates were inspected for cry proteins common to B. thuringiensis as well as toxins specific to B. cereus. No significant hits were found to any known cry genes while full-length matches were found for multiple B. cereus toxin genes. Finally the isolates were closely screened against B. anthracis genomes and no B. anthracis signatures were identified. Notably all isolates contained the plcR ancestral C allele and lacked significant hits to pXO1 and pXO2 plasmids and toxins. The collective results from the analysis confirm that these strains belong to a previously uncharacterized Bacillus species hereafter referred to as Bacillus issensis. The type strain is ISSFR-003T (=NRRL B-65389T= DSMZ 101676T).

Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene uptake was tracked over time and reported in T1_soil_microcosm_v2.0.csv. DNA was extracted from soil microcosm samples for microbial community analysis based on 16S rRNA gene sequencing; the resulting operational taxonomic units are presented in T2_soil_OTU_v2.0.csv. Bradyrhizobium sp. strain I71 was isolated from the soil microcosms and acetylene uptake and cell growth data for the isolate over time are shown in T3_soil_isolate_v2.0.csv. Nitrogen fixation assays for the pure culture of Bradyrhizobium sp. strain I71 are reported in T4_N2_fixation_v2.0.csv. Acetylene concentrations and cell densities from acetylenotrophic and heterotrophic growth assays for Bradyrhizobium sp. strain I71 are reported in T5_GrowthCurve_v2.0.csv

Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene uptake was tracked over time and reported in T1_soil_microcosm_v2.0.csv. DNA was extracted from soil microcosm samples for microbial community analysis based on 16S rRNA gene sequencing; the resulting operational taxonomic units are presented in T2_soil_OTU_v2.0.csv. Bradyrhizobium sp. strain I71 was isolated from the soil microcosms and acetylene uptake and cell growth data for the isolate over time are shown in T3_soil_isolate_v2.0.csv. Nitrogen fixation assays for the pure culture of Bradyrhizobium sp. strain I71 are reported in T4_N2_fixation_v2.0.csv. Acetylene concentrations and cell densities from acetylenotrophic and heterotrophic growth assays for Bradyrhizobium sp. strain I71 are reported in T5_GrowthCurve_v2.0.csv

This web application dataset includes data collected by the U.S. Geological Survey (USGS) as well as environmental climatic, geochemical, and mineralogical variables from various sources. NOTE: This web application is no longer being supported, and has been removed from ArcGIS Online as of September 30, 2023. Please see the Process Steps of this metadata record for more information. Layers include: U.S. Boundary Layers (States, Counties, Watersheds, and EPA Regions), Bacillus anthracis PCR results (rpoB, pXO1, pXO2 genetic markers), Outbreak Counties, NOAA U.S. Climate Normals for Precipitation 1981-2010 (inches per year), USDA Census Data and Non-Agricultural Bison Herd Population Data, Soil pH (SSURGO), Soil pH (STATSGO), Slope (SSURGO), Slope (STATSGO), Flood Frequency (SSURGO), Flood Frequency (STATSGO), Drainage Class (SSURGO), Drainage Class (STATSGO), USGS Soil Geochemistry (0-5 cm depth), USGS Soil Geochemistry (A-horizon), USGS Soil Geochemistry (C-horizon), NOAA NCDC - Average Precipitation, NOAA NCDC - Average Temperature, USGS 2012 100m National Elevation Dataset (NED), and the NDFD Real-Time 72 Hour Precipitation Forecast.

Continuous monitoring of bacterial community structure in the ISS-Kibo. This data contains numerous sequence reads of 16S rRNA gene fragments.

Draft genomes of various bacterial phyla isolated from different locations on the International Space Station as part of the Microbial Tracking 2 mission (ISS-MOP).

The BRIC-21 mission was designed to identify the response of Bacillus subtilis to the human spaceflight environment. For this mission samples were grown in rich-medium using the Biological Research in Canister Petri Dish Fixation Units (BRIC-PDFU) spaceflight hardware. B. subtilis spores were inoculated during spaceflight grown at the ambient ISS temperature and frozen in the onboard -80 C freezer prior to returning to Earth. RNA was extracted from samples grown onboard the International Space Station (ISS) and matching Ground Controls for transcriptome analysis.

Comparing the transcriptional responses of Bacillus subtilis strains WN624 and WN1106 at 5 kPa and 101 kPa. WN1106 is a 5 kPa-evolved strain with increased fitness compared to ancestor-WN624 strain at 5 kPa. This experiment probed the difference in response when the strains are grown at 5 kPa. Two-condition experiment 5 kPa vs. 101 kPa for both strains. And two component condition of WN1106 compared to WN624 at either 5 kPa or 101 kPa. 4 pressure comparisons.