Using Evironmental Samplers to Detect Ceratocystis Fungi in Orchidland 2016
공공데이터포털
Rapid ʽŌhiʽa Death (ROD) currently threatens ōhiʽa lehua (Metrosideros polymorpha) on Hawaiʽi Island. First identified in Puna in 2014, the disease has now spread island wide. Besides direct sampling of trees, environmental sampling could serve as an easier and broader strategy to detect Ceratocystis spp., the fungi causing Rapid Ohia Death (ROD). Envrionmental sampling could also help monitor the effect of felling ROD infected trees. We developed Passive and Active Environmental Samplers for collecting airborne particulates and deployed them at a property in Puna, where both C. lukuohia, and C. huliohia had been detected, and where the land owner practiced the management method of felling infected trees. We set up 2 Active Environmental Samplers (modified mosquito traps connected to a battery that uses a fan to continuously draw in air) and 3 Passive Environmental Samplers (uses a vane to move in the direction of the wind without the use of electricity) from July 12th to October 25th, 2016. The Active Traps contained one slide (1 replicate) each, while the Passive Traps contained 4 slides (4 replicates) each. Wind and precipitation data from a National Oceanic and Atmospheric Agency (NOAA) weather station at the Hilo airport was used in analysis. The dataset contains a list of sampling weeks, their start and end dates, and whether or not tree felling occurred during that week.
Using Evironmental Samplers to Detect Ceratocystis Fungi in Orchidland 2016
공공데이터포털
Rapid ʽŌhiʽa Death (ROD) currently threatens ōhiʽa lehua (Metrosideros polymorpha) on Hawaiʽi Island. First identified in Puna in 2014, the disease has now spread island wide. Besides direct sampling of trees, environmental sampling could serve as an easier and broader strategy to detect Ceratocystis spp., the fungi causing Rapid Ohia Death (ROD). Envrionmental sampling could also help monitor the effect of felling ROD infected trees. We developed Passive and Active Environmental Samplers for collecting airborne particulates and deployed them at a property in Puna, where both C. lukuohia, and C. huliohia had been detected, and where the land owner practiced the management method of felling infected trees. We set up 2 Active Environmental Samplers (modified mosquito traps connected to a battery that uses a fan to continuously draw in air) and 3 Passive Environmental Samplers (uses a vane to move in the direction of the wind without the use of electricity) from July 12th to October 25th, 2016. The Active Traps contained one slide (1 replicate) each, while the Passive Traps contained 4 slides (4 replicates) each. Wind and precipitation data from a National Oceanic and Atmospheric Agency (NOAA) weather station at the Hilo airport was used in analysis. The dataset contains a list of sampling weeks, their start and end dates, and whether or not tree felling occurred during that week.
ROD tree description, Hawaii, 2019
공공데이터포털
This data release includes metadata and tabular data that describes the location, diameter and treatment date for each ohia (Metrosideros polymorpha) involved in the study. Overall, 27 trees were selected for inclusion in the study. Six were inoculated with Ceratocystis lukuohia and three were not inoculated and acted as controls, at each of three study sites.
Hawaiʻi Island Rapid ʻŌhiʻa Death Ambrosia Beetle Communities and Frass 2018-2019
공공데이터포털
This data release includes data and metadata containing (1) study site locations and elevation where bolts (tree stem sections) infected with Ceratocystis lukuohia and/or C. huliohia were collected on Hawai'i Island, (2) the ambrosia beetle (Coleoptera: Scolytinae) species that were reared from bolts, (3) Ceratocystis spp. viability results from culturing tests of the beetles' frass, (4) frass production estimates from three ambrosia beetle species, and (5) quantitative Polymerase Chain Reaction (qPCR) DNA results of viability subcultures. Data were used in the analysis for the manuscript "Ambrosia beetle communities and frass production in ʻōhiʻa (Metrosideros polymorpha) infected with Ceratocystis fungi responsible for Rapid ʻŌhiʻa Death." Beetles and frass were collected in the lab from Rapid 'Ōhiʻa Death-infected trees from 2018-2019.