This dataset contains information on the Bullseye Snakehead fish found only in southeastern Florida. It is a subset of a larger database, the Nonindigenous Aquatic Species Database (NAS). This information resource is an established central repository for spatially referenced biogeographic accounts of introduced aquatic species. The NAS website provides scientific reports, online/real-time queries, spatial data sets, distribution maps, fact sheets, and general information.

This dataset contains information on the Bullseye Snakehead fish found only in southeastern Florida. It is a subset of a larger database, the Nonindigenous Aquatic Species Database (NAS). This information resource is an established central repository for spatially referenced biogeographic accounts of introduced aquatic species. The NAS website provides scientific reports, online/real-time queries, spatial data sets, distribution maps, fact sheets, and general information.

The data were collected by U.S.G.S. biologists and their partners during several field trips from March 2013 to April 2014 in Guantanamo Bay, Cuba. Data were collected during the manatee health assessments. The U.S. Geological Survey’s Sirenia Project is a federal research effort focusing on manatee biology and ecology with a long history of studies in the U.S., Puerto Rico, and the international arena. The status of the Antillean manatee in Cuba is not well known. Manatee population census techniques are mostly prohibited due to regulation of the airspace, but it is speculated that the population of manatee in Cuba is not very large. Manatee captures will enable health assessments and radio tagging to determine movement patterns.

West Indian manatees (Trichechus manatus) are separated into two allopatric subspecies: the Florida manatee (T. m. latirostris) and the Antillean manatee (T. m. manatus). In the winter of 2020-2021, an adult manatee was sighted off the coast of Cancun, Quintana Roo, Mexico, in areas where Antillean manatees are not typically seen. The individual had distinct watercraft scars on its body, which were matched using photo-identification to a known male Florida manatee (PE424) that had been repeatedly photographed in Florida since 1998. The data presented are the verified photo-documented sightings history of PE424 from Florida and Mexico.

Bullseye snakehead, Channa marulius, was first detected in 2000 in the southern Florida town of Tamarac and has been expanding its geographic range. Environmental DNA (eDNA) analysis is a newly-developed technique used to non-invasively detect cryptic or low-density species, or those that are logistically difficult to study. Genetic material shed into the environment through tissue and body fluids is concentrated from water samples and analyzed for the presence of target species eDNA. To help delineate bullseye snakehead’s geographic range, we developed and validated a species-specific eDNA assay for both quantitative and droplet digital PCR (ddPCR). We then used ddPCR to assess 16 locations in southeast Florida using 222 water samples collected from 2015-2018. Positive eDNA detections were obtained at all six locations that were within the known geographic range of bullseye snakehead. Furthermore, eDNA was detected in six of 10 locations that were previously thought to be outside the periphery of the range but hydrologically connected through the extensive canal system. Over the four years of sampling, estimated occurrence rates (ψ) remained stable and relatively high (ψ = 0.67 [95% Confidence Interval (CI) 0.33-0.95]) near Tamarac, FL, as compared to the most southern sampling sites (ψ = 0.0-0.37). Bullseye snakehead eDNA estimated occurrence rates in the middle region increased between 2016 (0.28 [95% CI 0.03-0.94]) and 2017 (0.66 [95% CI 0.24-0.98]), potentially reflecting eDNA detections related to a growing or expanding population. Bullseye snakehead eDNA was detected at low concentrations on the northern and eastern borders of Everglades National Park, which is an important conservation area and UNESCO World Heritage Site. Despite extensive sampling via electrofishing, no bullseye snakeheads were visually detected in several locations that yielded positive eDNA samples. It is unclear whether eDNA was transported through flowing water or another vector. To date, collection records for this species are confined to urban canals; however, bullseye snakehead may use the interconnected system of canals to disperse to natural conservation areas, such as Everglades National Park, Big Cypress National Preserve and Water Conservation Areas, where it may impact native species via predation and competition.

Bullseye snakehead, Channa marulius, was first detected in 2000 in the southern Florida town of Tamarac and has been expanding its geographic range. Environmental DNA (eDNA) analysis is a newly-developed technique used to non-invasively detect cryptic or low-density species, or those that are logistically difficult to study. Genetic material shed into the environment through tissue and body fluids is concentrated from water samples and analyzed for the presence of target species eDNA. To help delineate bullseye snakehead’s geographic range, we developed and validated a species-specific eDNA assay for both quantitative and droplet digital PCR (ddPCR). We then used ddPCR to assess 16 locations in southeast Florida using 222 water samples collected from 2015-2018. Positive eDNA detections were obtained at all six locations that were within the known geographic range of bullseye snakehead. Furthermore, eDNA was detected in six of 10 locations that were previously thought to be outside the periphery of the range but hydrologically connected through the extensive canal system. Over the four years of sampling, estimated occurrence rates (ψ) remained stable and relatively high (ψ = 0.67 [95% Confidence Interval (CI) 0.33-0.95]) near Tamarac, FL, as compared to the most southern sampling sites (ψ = 0.0-0.37). Bullseye snakehead eDNA estimated occurrence rates in the middle region increased between 2016 (0.28 [95% CI 0.03-0.94]) and 2017 (0.66 [95% CI 0.24-0.98]), potentially reflecting eDNA detections related to a growing or expanding population. Bullseye snakehead eDNA was detected at low concentrations on the northern and eastern borders of Everglades National Park, which is an important conservation area and UNESCO World Heritage Site. Despite extensive sampling via electrofishing, no bullseye snakeheads were visually detected in several locations that yielded positive eDNA samples. It is unclear whether eDNA was transported through flowing water or another vector. To date, collection records for this species are confined to urban canals; however, bullseye snakehead may use the interconnected system of canals to disperse to natural conservation areas, such as Everglades National Park, Big Cypress National Preserve and Water Conservation Areas, where it may impact native species via predation and competition.

Morphometric data (mass, length, circumference, age) collected during an observation of a radio-telemetered female Burmese python (Python bivitattus) that ingested an adult female white-tailed deer (Odocoileus virginianus), retained the deer within the digestive tract for 10 days, and then vomited the deer coinciding with a drop in air temperature.

This dataset provides morphological and blood-derived physiological data from invasive Burmese pythons (Python bivittatus) captured in Florida. Data were collected on pythons at capture in the field and in varying lengths of time in captivity. Samples were collected immediately at handling (baseline) and 1 hour post-restraint. Physiological data include circulating corticosterone hormone levels, functional immune assays, and blood cell counts. Also included is R code ( Python_captivity_capture_code.R ) to replicate analyses used in the publication "Scales of Stress: Effects of capture and captivity in an invasive snake". For reference, the following data files are relevant to each section of the corresponding manuscript: 1. Effects of different timescales of stressors: python_long.csv cellcountpart1.csv 2. Finer scale and non-linear relationships between metrics and captivity: python_wide.csv cellcountpart2.csv 3. Viral Infection Status: virus_data_full.csv

This dataset provides morphological and blood-derived physiological data from invasive Burmese pythons (Python bivittatus) captured in Florida. Data were collected on pythons at capture in the field and in varying lengths of time in captivity. Samples were collected immediately at handling (baseline) and 1 hour post-restraint. Physiological data include circulating corticosterone hormone levels, functional immune assays, and blood cell counts. Also included is R code ( Python_captivity_capture_code.R ) to replicate analyses used in the publication "Scales of Stress: Effects of capture and captivity in an invasive snake". For reference, the following data files are relevant to each section of the corresponding manuscript: 1. Effects of different timescales of stressors: python_long.csv cellcountpart1.csv 2. Finer scale and non-linear relationships between metrics and captivity: python_wide.csv cellcountpart2.csv 3. Viral Infection Status: virus_data_full.csv

We investigated fine-scale genetic patterns of the federally threatened Eastern Massasauga Rattlesnake (Sistrurus catenatus) on a relatively undisturbed island in northern Michigan, USA. This species often persists in habitat islands throughout much of its distribution due to extensive habitat loss and distance-limited dispersal. These data are from 102 individual Eastern Massasauga Rattlesnakes sampled at Bois Blanc Island, Michigan and genotyped at 15 microsatellite loci. Samples were collected as part of a study to examine functional connectivity for the Eastern Massasauga. We found that the entire island population exhibited weak genetic structuring with spatially segregated variation in effective migration and genetic diversity.