The Nuclear Magnetic Resonance Spectral Measurement Database (NMR-SMDB) was developed for the purpose of organizing and searching NMR spectral data of protein therapeutics, linking spectra to corresponding sample information and enabling quick access to full datasets and entire studies. In addition to supporting internal NIST research, the system could facilitate data access to stakeholders outside of NIST, and future versions of the database software itself could be installed by others for their own data storage and retrieval.

The NISTmAb Interlaboratory NMR Study is a joint effort between 25 organizations to evaluate the performance of two-dimensional (2D) heteronuclear NMR as used to characterize the high order structure (HOS) of proteins, with the aim of harmonizing 2D-NMR methods to support industrial use of NMR as applied to mAb therapeutics. This study features nearly equal representation between laboratories from academia, government, and industry, including 4 laboratories from regulatory agencies. In the study, each participating lab measured a series of NMR spectra on the NIST-Fab, which was derived by papain cleavage of the NISTmAb. A 20%-enriched 13C, uniformly enriched 15N NIST-Fab, produced from Pichia pastoris, served as the system suitability sample.This package contains all of the original time-domain data as measured by the collaborating partners, converted to NMRPipe format. It also includes corresponding spectra as processed at NIST, and the software scripts and processing parameters that were used to generate the spectra. The entire package is greater than 4 GB. The software NMRPipe, version 9.6 or later, is required for this data package.

국립암센터 대장암 분자병리 NRAS MUTATION 종류 데이터로 인증키, 페이지수, 결과코드, 기준년도, 목록리스트 등의 항목을 제공합니다. 국립암센터 대장암 분자병리 NRAS MUTATION 종류 공공데이터는 아래의 URL을 통해서도 신청 및 사용하실 수 있습니다. https://www.bigdata-cancer.kr/ncc/viewOpenApiDetail.do?datasetIdentifier=15240

Background NF1 refers to type 1 neurofibromatosis syndrome, which has been linked with mutations of the large NF1 gene. NF1 tumor suppressor protein, neurofibromin, has been shown to regulate ras: the NF1 protein contains a GTPase activating protein (GAP) related domain which functions as p21rasGAP. Our studies have previously demonstrated that the NF1 protein forms a high affinity association with cytokeratin 14 during the formation of desmosomes and hemidesmosomes in cultured keratinocytes. Methods The expression of NF1 protein was studied in developing human epidermis using western transfer analysis, indirect immunofluorescence, confocal laser scanning microscopy, immunoelectron microscopy, and in situ hybridization. Results The expression of NF1 protein was noted to be highly elevated in the periderm at 8 weeks estimated gestational age (EGA) and in the basal cells at 8–14 weeks EGA. During this period, NF1 protein was associated with cytokeratin filaments terminating to desmosomes and hemidesmosomes. NF1 protein did not display colocalization with α-tubulin or actin of the cytoskeleton, or with adherens junction proteins. Conclusions These results depict an early fetal period when the NF1 tumor suppressor is abundantly expressed in epidermis and associated with cytokeratin filaments. This period is characterized by the initiation of differentiation of the basal cells, maturation of the basement membrane zone as well as accentuated formation of selected cellular junctions. NF1 tumor suppressor may function in the regulation of epidermal histogenesis via controlling the organization of the keratin cytoskeleton during the assembly of desmosomes and hemidesmosomes.

,NeuroMorpho.Org is a centrally curated inventory of digitally reconstructed neurons associated with peer-reviewed publications. It contains contributions from over 80 laboratories worldwide and is continuously updated as new morphological reconstructions are collected, published, and shared.,

This is the taxonomy that is used for classifying information on the NIST website. It comprises a set of top-level designations that include the research areas within NIST, with two levels of subcategories beneath.

,dbSNP is a database of single nucleotide polymorphisms (SNPs) and multiple small-scale variations that include insertions/deletions, microsatellites, and non-polymorphic variants.,

,Gene Expression Omnibus is a public functional genomics data repository supporting MIAME-compliant submissions of array- and sequence-based data. Tools are provided to help users query and download experiments and curated gene expression profiles.,

This dataset was utilized to evaluate associations between accelerated epigenetic and transcriptomic age and NMR-measured metabolic and inflammatory biomarkers. It contains information on biological and chronological age, confounders, and NMR-measured 'omic profiles. This dataset is not publicly accessible because: The data is derived from medical records which cannot be disclosed per federal law (HIPPA) and existing data use agreements. It can be accessed through the following means: The data can be accessed via sending a request to the CATHGEN Executive Committee (https://dmpi.duke.edu/studies/cathgen). An approved IRB application may be required by the committee. Format: The data is tabular data containing columns specifying the epigenetic and transcriptomic age of the individuals along with NMR-measured metabolic biomarkers. Social and demographic confounders are are included within the dataset. This dataset is associated with the following publication: Raab, H., E. Hauser, L. Kwee, S. Shah, W. Kraus, and C. Ward-Caviness. Associations among NMR-measured inflammatory and metabolic biomarkers and accelerated aging in cardiac catheterization patients. Aging. Impact Journals, LLC, Orchard Park, NY, USA, 16(8): 6652-6672, (2024).

,The NIDDK Central Repository stores biosamples, genetic and other data collected in designated NIDDK-funded clinical studies. The purpose of the NIDDK Central Repository is to expand the usefulness of these studies by allowing a wider research community to access data and materials beyond the end of the study.,