,Modern swine production facilitates indoor respiratory contact between human employees and pigs in their care, creating conditions for interspecies transmission of influenza A virus (IAV). Sow vaccination is routinely practiced in the U.S.A. to transfer maternal derived antibodies (MDA) to piglets. Weaning is a highly stressful period for piglets that requires increased human interaction. Weaned piglets potentially have mixed immunity from MDA: matched, mismatched, and naïve. Since there have been multiple introductions of human seasonal H3N2 to swine, we assessed the effect of matched and mismatched MDA acquired from vaccinated sows and the stress of weaning on the susceptibility of piglets to a human-origin H3N2 IAV. The H3N2 virus was generated by reverse genetics to mimic the 2010.1 H3N2 introduction from humans to swine. Challenged seeder piglets were divided by immune and weaning status. Two days post inoculation, naïve direct contact pigs were placed with seeders. IAV quantitative reverse transcription polymerase chain reaction (qRT-PCR) and virus titration were performed on nasal swabs and bronchoalveolar lavage fluid to evaluate shedding and transmission kinetics. Matched MDA were effective in reducing shedding in challenged pigs and minimizing transmission of the human-like H3N2 to contacts. There was an increase in shedding and transmission in weaned pigs compared to littermates that remained on the sow. These results identify critical control points in production where changing practices could mitigate human-to-swine and swine-to-swine transmission to prevent establishment of novel lineages in pig populations.,

,The dataset contains measurements from a swine research study evaluating influenza A immune responses and protection for replicon particle and live attenuated influenza virus vaccines. The research study was conducted by United States Department of Agriculture, Agricultural Research Service (USDA-ARS) scientists and postdoctoral scientists at the National Animal Disease Center, USDA-ARS to characterize heterologous virus immunity from live attenuated influenza A virus vaccines and IAV replicon particle vaccines. A better understanding of vaccine immune responses to heterologous viruses will aid in development of improved swine IAV vaccination strategies. The dataset contains lesion scores, virus shedding in nasal swabs and bronchoalveolar lavage fluid, serum and bronchoalveolar lavage fluid antibody responses, and isolated blood and lung T cell responses.,

,Groundwater samples were collected in an observational study design by dead-end ultrafiltration from private wells on 40 Iowa swine farms and analyzed by quantitative polymerase chain reaction (qPCR) to assess contamination by endemic swine pathogens and swine manure markers. These data facilitate investigation of groundwater as a biosecurity risk on swine farms. Each farm was sampled one time. Twenty farms were sampled in spring of 2024 (4/15/2024 – 5/29/2024) and twenty farms were sampled in fall of 2024 (9/16/2024 – 10/29/2024). Sample volumes were 639–853 L (mean = 759). Control samples were collected in the field for each field sample, and control samples were tested for all organisms if the corresponding field sample tested positive for any organism. Samples were shipped on ice to the laboratory where they were backflushed, underwent secondary concentration, and archived at -80 degrees Celsius. Secondary concentrates were subsampled for nucleic acid extraction using the QIAGEN QIAcube Connect system, and each nucleic acid extract was analyzed in duplicate by qPCR using a Roche LightCycler 480 II for the following microorganisms: Cryptosporidium spp., enteropathogenic Escherichia coli, porcine circovirus type 2, porcine epidemic diarrhea virus, porcine reproductive and respiratory syndrome virus, rotavirus group C, Salmonella spp., swine Bacteroidales (2 qPCR assays), and swine influenza virus. Negative and positive controls were included at lab steps for concentration, nucleic acid extraction, reverse transcription, and qPCR. PCR inhibition was assessed in each nucleic acid extract and mitigated by dilution if necessary. Data are expressed as genomic copies per liter of groundwater sampled unless otherwise indicated. Dataset consists of 1 spreadsheet file: Dataset 01102025_V4.csv. Variables in this file are described in the included data dictionary.,

Emergence of avian influenza viruses with high lethality to humans, such as the currently circulating highly pathogenic A(H5N1) and A(H7N9) cause serious concern for the global economic and public health sectors. To improve our understanding of the spatial and temporal trends in transmission risk we developed a model that predicts hotspots of novel virus emergence between domestic and wild birds and incorporates H5N1 risk factors. Models were produced at 30 km spatial resolution across two temporal seasons. Files are named using a TransmisisonDirection_Season_DataType format such that Transmission direction can end in a (poultry to waterfowl) or b (waterfowl to poultry), Season can be breeding (br) or wintering (wi), and data type can be the mean predicted transmission (mn) or the coefficient of variation (cv).

Emergence of avian influenza viruses with high lethality to humans, such as the currently circulating highly pathogenic A(H5N1) and A(H7N9) cause serious concern for the global economic and public health sectors. To improve our understanding of the spatial and temporal trends in transmission risk we developed a model that predicts hotspots of novel virus emergence between domestic and wild birds and incorporates H5N1 risk factors. Models were produced at 30 km spatial resolution across two temporal seasons. Files are named using a TransmisisonDirection_Season_DataType format such that Transmission direction can end in a (poultry to waterfowl) or b (waterfowl to poultry), Season can be breeding (br) or wintering (wi), and data type can be the mean predicted transmission (mn) or the coefficient of variation (cv).

This data layer depicts the relative susceptibility of poultry to the transmission of Avian Influenza from wild birds based upon poultry type and density for the contiguous United States.

This data layer depicts the relative susceptibility of poultry to the transmission of Avian Influenza from wild birds based upon poultry type and density for the contiguous United States.

Data set containing avian influenza A virus (IAV) sampling information for Emperor Geese in Alaska, 2015-2017. The data are in three tables: 1) collection data and IAV screening results from fecal samples at several sites in southwestern Alaska, 2) results of blocking enzyme-linked immunosorbent assay (bELISA) tests for IAV antibodies in blood serum collected from nesting female Emperor geese near the Manokinak River on the Yukon-Kuskokwim River Delta, and 3) results of influenza virus microneutralization assays to test for the specific IAV subtypes in the Manokinak samples.

This data set describes genomic sequence information from 2022 used to infer spatiotemporal trends pertaining to the introductions of highly pathogenic H5N1 avian influenza viruses into Alaska and spread among wild birds, backyard poultry, and mammals.