데이터셋 상세
미국
Pseudogymnoascus destructans survival at elevated temperatures – Artificial media count data
The survival of Pseudogymnoascus destructans (Pd) was evaluated at temperatures outside of its thermal range of growth on three different artificial growth media; Sabouraud dextrose agar (SD), brain-heart infusion agar (BHI), and brain-heart infusion agar supplemented with 10% sheep red blood cells (BHI+B). Pd was harvested from starting cultures grown of MEA agar at 7˚C for 60 days. Harvested conidia were diluted in Phosphate Buffered Saline + Tween20 and spread onto plates of a given medium. Plate were then incubated at either 24, 30 or 37˚C. Plates were incubated for 1, 5, 9, 15, 30, 60, 90, 120, or 150 days before being transferred to a 7˚C incubator for 50 days. Colony forming units (CFUs) of Pd were then enumerated, resulting in a time series of Pd survival on a given medium at a given temperature. As each medium was inoculated from a different starting culture of Pd, a control group for each medium was created by inoculating plates as above and then immediate incubation at 7˚C for 50 days. The number of CFUs on the control plates was used as a statistical offset factor which allowed for the fair comparison of Pd survival between different media. The number of conidia initially plated onto each plate varied between 100, 1000 , and 10,000, depending on the temperature-medium treatment combination. In order to ensure robust statistical analysis, all data was rescaled by an appropriate correction factor which is also presented in the datafile.
데이터 정보
연관 데이터
Determinants of Pseudogymnoascus destructans within bat hibernacula: data
공공데이터포털
This dataset includes data used to summarize trends and identify best-fit models to explain patterns in presence-absence and abundance of Pseudogymnoascus destructans (Pd) in environmental substrates and on bats within six bat hibernacula at different stages of white-nose syndrome (WNS). Data relating to environmental substrates include: dates and relative spatial locations of samples collected within study hibernacula, presence and quantity of Pd in samples based on qPCR analysis, and daily temperature parameters at each sample location on the days samples were collected. Data relating to bats include: dates and relative spatial locations of hibernating bats that were sampled, species, sex, weight(g), forearm length(mm), body mass index (weight/forearm), proportion of the wing with visible fungus or fluorescence characteristic of WNS under hand-held UVA light and presence and quantity of Pd in wing-skin swab samples based on qPCR analysis. Measures of time since first detection of WNS at each study hibernaculum are also included in the dataset.
Protein predictions for Calonectria pseudonaviculata CBS 139707 (aka cpsCT01)
공공데이터포털
,Boxwood blight disease, caused by the fungi Calonectria henricotiae and C. pseudonaviculata, is an emergent threat to natural and managed landscapes worldwide. This dataset contains protein predictions and identifications generated from Calonectria pseudonaviculata CBS 139707 (aka cpsCT01) genome dataset https://doi.org/10.15482/USDA.ADC/1410184.,,
Data from the development and evaluation of a CRISPR/Cas12a-based biosensor for the detection of DNA from Pseudogymnoascus destructans using samples collected from various species of bats across the United States, from 2018 to 2023
공공데이터포털
Reported here are the fluorescence data points recorded from CRISPR/Cas12a biosensor assays to detect the presence or absence of DNA from Pseudogymnoascus destructans (Pd), the causative fungal agent of white-nose syndrome in bats, from artificial controls, dermal swab, and guano samples. Ct values from qPCR experiments are also provided in a table. Furthermore, we also provide the GenBank accession numbers used to develop the aforementioned CRISPR/Cas12a biosensor assay for Pd DNA detection.
Data from: Changes in plant architecture in Brazilian peppertree damaged by the biological control agent, Pseudophilothrips ichini Hood (Thysanoptera: Phlaeothripidae)
공공데이터포털
,These data come from a roughly 4-year study on the growth response of a highly invasive woody plant to the damage induced by its biological control agent (an insect) and to soil fertilizer levels. The study design was an interrupted time series in which data were collected from plants for 2 years ("pre"), agents were introduced, and data collection continued for 2 years with sustained agent releases ("post"). Each month, stem tip counts and other measurements were collected from roughly 200 plants: 100 in each of two garden plots (IPRL and UF/IRREC). As plants grew larger (eventually to ~250 cm tall), data collection was spaced to roughly every 2 months and some replicates within each treatment were dropped. In addition to the "interruption" of introducing the agents, the other treatment was the addition of various concentrations of liquid fertilizer to the soil throughout the study. While there were no control plants that did not receive insects, each plant was followed over the course of the pre-post design experiment and Bayesian mixed modelling was used to interpret the effect of releasing the insect on how plant growth parameters changed over time and under different fertilizer levels. We have included raw data in .csv and .xlsx formats, one set for each garden plot as plots were analyzed separately. The Excel files have notes in the header column providing more information about each variable. However, not all variables were analyzed in the published study. The R code is also attached, which runs the Bayesian analyses for each plot and each response variable of interest in the published study. Raw data are also provided for soil and foliar nitrogen content. Soil nitrogen was analyzed at both plots, but foliar nitrogen was only analyzed at one of the plots (IPRL).,
Post-fire vegetation cover, plant species diversity, and Ustilago bullata infection rates at Boise River Wildlife Management Area 2018-2019
공공데이터포털
Invasive-plant treatments often target a single or few species, but many landscapes are diversely invaded. Exotic annual grasses (EAGs) increase wildfires and degrade native perennial plant communities in cold-desert rangelands, and herbicides are thus sprayed to inhibit EAG germination and establishment. We asked how EAG-target and nontarget species responded to an herbicide mixture sprayed over a large, topographically diverse landscape after wildfire. We focused on how whole-community and natural EAG-pathogen treatment responses varied over years and physical properties of sites. We monitored plant cover and diversity in 41 pairs of plots located inside or outside areas (486 ha total) treated with a combined aerial broadcast spray of pre-emergent herbicide (imazapic) and weed-suppressive bacteria (Pseudomonas fluorescens, “MB906”) to target EAGs after wildfires in southwest Idaho, USA.