Pesticide concentrations determined in honey bee hive matrices from GC/qToF-MS analysis. Including neonicotinoid concentrations determined by LC-MS/MS as published in Lin et al., 2021. This dataset is associated with the following publication: Glinski, D., S. Purucker, J. Minucci, R. Richardson, C. Lin, R. Johnson, and W. Henderson. Analysis of contaminant residues in honey bee hive matrices. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 954: 176329, (2024).

Data on the presence of corn seed treatment insecticides in bee-collected pollen and increased honey bee mortality associated with corn planting, persistence of the insecticides inside honey bee colonies, and long-term growth of these colonies in central Ohio. We also constructed spatial models, based on empirical data of honey bee foraging and dispersion patterns of planter dust, and landscape compositions, to simulate hypothesized exposure routes via contamination of foraging resources and aerial exposure resulting from flight through localized dust plumes from planters and diffuse dust in the landscape over all resulting from widespread planting activity. Insecticide concentrations under different hypothesized exposure routes were then compared with the observed levels of contamination to evaluate these hypotheses. This dataset is associated with the following publication: Kuan, C., G. DeGrandi-Hoffman, R. Curry, K. Garber, A. Kanarek, M. Snyder, K. Wolfe, and T. Purucker. Sensitivity analyses for simulating pesticide impacts on honey bee colonies. ENVIRONMENTAL MODELLING AND SOFTWARE. Elsevier Science Ltd, New York, NY, USA, 376: 15-27, (2018).

Data on the presence of corn seed treatment insecticides in bee-collected pollen and increased honey bee mortality associated with corn planting, persistence of the insecticides inside honey bee colonies, and long-term growth of these colonies in central Ohio. We also constructed spatial models, based on empirical data of honey bee foraging and dispersion patterns of planter dust, and landscape compositions, to simulate hypothesized exposure routes via contamination of foraging resources and aerial exposure resulting from flight through localized dust plumes from planters and diffuse dust in the landscape over all resulting from widespread planting activity. Insecticide concentrations under different hypothesized exposure routes were then compared with the observed levels of contamination to evaluate these hypotheses. This dataset is associated with the following publication: Kuan, C., G. DeGrandi-Hoffman, R. Curry, K. Garber, A. Kanarek, M. Snyder, K. Wolfe, and T. Purucker. Sensitivity analyses for simulating pesticide impacts on honey bee colonies. ENVIRONMENTAL MODELLING AND SOFTWARE. Elsevier Science Ltd, New York, NY, USA, 376: 15-27, (2018).

,Honey bees (Apis mellifera) often encounter a variety of stressors in their environment, including poor nutrition and pesticides. These stressors interact and can be exacerbated in large-scale agroecosystems. We investigated how diets varying in macronutrient ratios can affect nurse bee susceptibility to pesticide stressors. Nurse bees were fed trace concentrations of clothianidin (CLO), a neonicotinoid insecticide known to have sublethal and lethal effects on honey bees, after newly emerged bees were given diets varying in proteins and lipids, a natural pollen diet, or sucrose solution diet. Bees given pollen had improved longevity, physiology, enzyme activity, and gene expression related to pesticide detoxification. The artificial diets helped improve bee health and physiology but did little to promote bee detoxification enzymes and genes. There was no effect of the trace CLO treatments on its own, but there was an interactive effect between our higher CLO treatment and poor nutrition on bee longevity and vitellogenin expression. Our results suggest that 1.) exposure to even trace concentrations of CLO can interact with poor nutrition to undermine adult bee health and 2.) macronutrients in artificial diets can help promote bee physiology, but other nutrients in pollen, such as potentially phytochemicals, are more directly linked honey bee tolerance to pesticide stress.,,This work was supported by United States Department of Agriculture, Agricultural Research Service, Research Project No. 6066-21000-001-000-D, Ecological Assessment and Mitigation Strategies to Reduce the Risks of Bees to Stressors in Southern Crop Ecosystems.,

Dataset for figures included in associated publication. This dataset is associated with the following publication: Lehmann, D., M. Batres, and W. Williams. Impact of Diflubenzuron on Bombus Impatiens Microcolony Development. ENVIRONMENTAL ENTOMOLOGY. Entomological Society of America, Lantham, MD, USA, 49(1): 203-210, (2020).

Dataset for figures included in associated publication. This dataset is associated with the following publication: Lehmann, D., M. Batres, and W. Williams. Impact of Diflubenzuron on Bombus Impatiens Microcolony Development. ENVIRONMENTAL ENTOMOLOGY. Entomological Society of America, Lantham, MD, USA, 49(1): 203-210, (2020).

,Honey bees (Apis mellifera) often encounter a variety of stressors in their environment, including poor nutrition and pesticides. These stressors interact and can be exacerbated in large-scale agroecosystems. We investigated how diets varying in macronutrient ratios can affect nurse bee susceptibility to pesticide stressors. Nurse bees were fed sublethal concentrations of clothianidin (CLO), a neonicotinoid insecticide known to have sublethal and lethal effects on honey bees, after newly emerged bees were given diets varying in proteins and lipids, a natural pollen diet, or no diet. Bees given pollen had improved longevity, physiology, enzyme activity, and gene expression related to pesticide detoxification. The artificial diets helped improve bee health and physiology but did little to promote bee detoxification enzymes and genes. There was no effect of the sublethal CLO treatments on its own, but there was an interactive effect between our high CLO treatment and nutrition on bee longevity and vitellogenin expression. Honey bees given pollen upregulated of detoxification genes and had higher enzyme activity related to pesticide metabolism. Our results suggest that macronutrients in artificial diets can help promote bee physiology, but other nutrients in pollen, such as potentially phytochemicals, are more directly linked honey bee tolerance to pesticide stress.,,This work was supported by United States Department of Agriculture, Agricultural Research Service, Research Project No. 6066-21000-001-000-D, Ecological Assessment and Mitigation Strategies to Reduce the Risks of Bees to Stressors in Southern Crop Ecosystems.,