These data include raw sequencing data (bacterial 16S and eukaryote 18S rRNA genes) and a summary result table for bacteria classification in an Excel file. This dataset is associated with the following publication: Hwang, J., H. Ryu, K. Rodriguez, S. Fahad, J. SantoDomingo, A. Kushima, and W. Lee. A strategy for power generation from bilgewater using a photosynthetic microalgal fuel cell (MAFC). JOURNAL OF POWER SOURCES. Elsevier Science Ltd, New York, NY, USA, 484: 229222, (2021).

Sequence reads (16S rDNA- and 16S rRNA-based) were processed and analyzed using Mothur software. The results presented in the attached Excel file. Also, the other MS word file includes taxonomic summary tables for bacterial communities on biofilms from the MAIFAS reactor as well as the detailed description of Materials & Methods. This dataset is associated with the following publication: Church, J., H. Ryu, A. Sadmani, A. Randall, J. Santodomingo, and W.H. Lee. Multiscale investigation of a symbiotic microalgal-integrated fixed film activated sludge (MAIFAS) process for nutrient removal and photo-oxygenation. Bioresource Technology. Elsevier Online, New York, NY, USA, 268: 128-138, (2018).

Sequence reads (16S rDNA- and 16S rRNA-based) were processed and analyzed using Mothur software. The results presented in the attached Excel file. Also, the other MS word file includes taxonomic summary tables for bacterial communities on biofilms from the MAIFAS reactor as well as the detailed description of Materials & Methods. This dataset is associated with the following publication: Church, J., H. Ryu, A. Sadmani, A. Randall, J. Santodomingo, and W.H. Lee. Multiscale investigation of a symbiotic microalgal-integrated fixed film activated sludge (MAIFAS) process for nutrient removal and photo-oxygenation. Bioresource Technology. Elsevier Online, New York, NY, USA, 268: 128-138, (2018).

These data are bacterial 16S rRNA sequences and a taxonomic summary table for biofilm samples from the bio-reactors. The data may provide background/supporting information for other researchers who have a similar experimental plan with a microbial electrochemical cell reactor. This dataset is associated with the following publication: Santodomingo, J., H. Lee, B. Dhar, J. An, B. Rittmann, H. Ren, and J. Chae. The Roles of Biofilm Conductivity and Donor Substrate Kinetics in a Mixed-Culture Biofilm Anod. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 50(23): 12799-12807, (2016).

These data are bacterial 16S rRNA sequences and a taxonomic summary table for biofilm samples from the bio-reactors. The data may provide background/supporting information for other researchers who have a similar experimental plan with a microbial electrochemical cell reactor. This dataset is associated with the following publication: Santodomingo, J., H. Lee, B. Dhar, J. An, B. Rittmann, H. Ren, and J. Chae. The Roles of Biofilm Conductivity and Donor Substrate Kinetics in a Mixed-Culture Biofilm Anod. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 50(23): 12799-12807, (2016).

Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene uptake was tracked over time and reported in T1_soil_microcosm_v2.0.csv. DNA was extracted from soil microcosm samples for microbial community analysis based on 16S rRNA gene sequencing; the resulting operational taxonomic units are presented in T2_soil_OTU_v2.0.csv. Bradyrhizobium sp. strain I71 was isolated from the soil microcosms and acetylene uptake and cell growth data for the isolate over time are shown in T3_soil_isolate_v2.0.csv. Nitrogen fixation assays for the pure culture of Bradyrhizobium sp. strain I71 are reported in T4_N2_fixation_v2.0.csv. Acetylene concentrations and cell densities from acetylenotrophic and heterotrophic growth assays for Bradyrhizobium sp. strain I71 are reported in T5_GrowthCurve_v2.0.csv

The excel spreadsheet includes sample IDs and labeling information for DNA sequencing raw data. In addition, DNA concentrations for all the biofilm samples analyzed are presented. This dataset is associated with the following publication: Li, L., Y. Jeon, S. Lee, H. Ryu, J. Santodomingo, and Y. Seo. Dynamics of the Physiochemical and Community Structures of Biofilms under the Influence of Algal Organic Matter and Humic Substances. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 158: 136-145, (2019).

Data in these tables appear in the manuscript and were used to produce the published figures. This dataset is associated with the following publication: Wu, S., M. Yassine, M. Suidan, and A. Venosa. Anaerobic Biodegradation of soybean biodiesel and diesel blends under sulfate-reducing conditions. Jacob de Boer, Shane Snyder CHEMOSPHERE. Elsevier Science Ltd, New York, NY, USA, 161: 382-389, (2016).

Data used to generate Figures 2, 3, and 4 as we ll as Table 3. This dataset is associated with the following publication: Banerji, A., M. Bagley, J. Shoemaker, D. Tettenhorst, C. Nietch, J. Allen, and J. Santodomingo. Evaluating putative ecological drivers of microcystin spatiotemporal dynamics using metabarcoding and environmental data. Harmful Algae. Elsevier B.V., Amsterdam, NETHERLANDS, 86: 84-95, (2019).