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Background The ability of Mycobacterium tuberculosis to survive and replicate in macrophages is crucial for the mycobacterium's ability to infect the host and cause tuberculosis. To identify Mycobacterium tuberculosis genes involved in survival in macrophages, a library of non-pathogenic Mycobacterium smegmatis bacteria, each carrying an individual integrated cosmid containing M. tuberculosis H37Rv genomic DNA, was passed through THP-1 human macrophages three times. Results Two of the clones recovered from this enrichment process, sur2 and sur3, exhibited significantly increased survival relative to wild-type bacteria. In coinfection experiments, the ratio of sur2 colonies to wild-type colonies was 1:1 at 0 hours but increased to 20:1 at 24 hours post phagocytosis. The ratio of sur3 colonies to wild-type colonies was 1:1 at 0 hours and 5:1 at 24 hours. The M. tuberculosis ORFs responsible for increased survival were shown to be Rv0365c for the sur2 clone and Rv2235 for the sur3 clone. These ORFs encode proteins with as-of-yet unknown functions. Conclusions We identified two M. tuberculosis ORFs which may be involved in the ability of tubercle bacilli to survive in macrophages.

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This product consists of multiple tabular datasets and associated metadata for clinical status, gene transcripts, and lab results related to presence of Mycoplasma spp. in desert reptiles. To better understand immune responses to pathogenic infections, we conducted an experiment to quantify innate and induced immune responses using gene transcript profiles and measured induced antibody levels for Mycoplasma spp. in Mojave Desert tortoises (Gopherus agassizii). Data consists of: - Clinical Status Data - Clinical status of presence or absence of nasal discharge, eroded nares, or labored respiration in 15 captive tortoises classified as naive, exposed, or infected. - Gene Transcript Data - Gene transcription values for 11 genes of interest for 14 adult captive male tortoises and 13 adult wild tortoises in Clark County, Nevada, USA. - Laboratory Data - Disease laboratory results for qPCR and ELISA test for five control, five exposed, and five infected captive adult tortoises and 14 reference wild adult tortoises in Clark County, Nevada, USA.

This product consists of multiple tabular datasets and associated metadata for clinical status, gene transcripts, and lab results related to presence of Mycoplasma spp. in desert reptiles. To better understand immune responses to pathogenic infections, we conducted an experiment to quantify innate and induced immune responses using gene transcript profiles and measured induced antibody levels for Mycoplasma spp. in Mojave Desert tortoises (Gopherus agassizii). Data consists of: - Clinical Status Data - Clinical status of presence or absence of nasal discharge, eroded nares, or labored respiration in 15 captive tortoises classified as naive, exposed, or infected. - Gene Transcript Data - Gene transcription values for 11 genes of interest for 14 adult captive male tortoises and 13 adult wild tortoises in Clark County, Nevada, USA. - Laboratory Data - Disease laboratory results for qPCR and ELISA test for five control, five exposed, and five infected captive adult tortoises and 14 reference wild adult tortoises in Clark County, Nevada, USA.