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Cruise NF-12-04-SERA (Sanctuary Regional Development and Assessment) (Reson7125). 1)Ship based mapping and characterization of benthic habitats in the waters around Gray’s Reef National Marine Sanctuary. Collected data will need to include backscatter.2)Continuation of invertebrate recruitment investigation in Gray’s Reef. This project will include diving to assess the colonization and succession of invertebrates to a natural substrate. 3)Continue investigations to quantify variation in space and time of the abundance ofschooling prey and mid-water predators at mid-shelf reefs: this involves conducting survey lines using the EK-60 survey system. 4)Continue collecting data on the abundance,diversity and distribution of both fishes and invertebrates both in side and outside the proposed Research Area in Gray’s Reef.This will require divers to conduct visual fis hcensuses along transects and take pictures of quadrats to assess invertebrate fauna. 5)Gray’s Reef would like to explore the possibility of conducting night dives aboard its own diving vessels provided that this does not present theNancy Fostercrew with unduestaffing/overtime issues and would be worked out in advance of conducting operations.

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Cruise NF-12-06-FKNMS (Florida Keys National Marine Sanctuary) (Reson7125). 1) Multibeam survey over Boca Grande Bar, the purported mutton spawning aggregation site near Western Dry Rocks. Couple with fishery acoustics, FL FWC’s ROV, divers or drop camera for habitat validation. 2)Multibeam target areas in the vicinity of the Dry Tortugas (DT Westbank Gaps) and northwest of Loggerhead Key (“The River”), including fisheries acoustics survey for fish biomass distribution. 3)Fisheries acoustics mapping in select region(s) bisected by the TNER boundary to contribute to existing data on distribution of fish biomass in relation to Ecological Reserve boundary. 4)Drop camera and/or diver visual surveys over bathymetry habitat features of interest, particularly in “The River”. 5)Service array of VR2 receivers in the vicinity of Dry Tortugas using divers. 6)Dive and lower camera over the side of ship or a small boaton mutton spawning aggregation locations on Riley’s Hump on/around August full moon on/around 2 August.

Mapping Essential Fish Habitat in the US Caribbean to Inform MPA Management (NF-15-01-USVI, Reson 7125). Scientists will collect high resolution multibeam and acoustic fisheries data in mid-water depths approximately 10 to 2000 meters so as to continue to characterize seafloor habitats within all U.S. States, Territories, and Commonwealths. The objective of this project is to collect a multibeam bathymetry dataset with 100% seafloor ensonification, along with multibeam backscatter suitable for seafloor characterization. Fishery acoustics data will be collected to characterize broad-scale fish abundance, biomass, and utilization patterns, as well as to locate and document fish spawning aggregations. Multibeam data will be collected to conform to IHO Order 1 (less than 100m) and Order 2 (greater than 100m) accuracy standards. The strategies developed for each survey area will take into account the minimum depths, general bathymetry, and time allotment. The delineation and identification of seafloor habitats will be assisted by the use of a moderate-depth Remotely Operated Vehicle (ROV). The vehicle has video and frame camera capability to depths of 300 meters and will be used for transect sampling within areas mapped during this mission. Two Slocum Glider G2 will be deployed during the duration of the project to collect oceanographic data and passive fish acoustics in the study region.

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