Concentrations of PMMoV and SARS-CoV-2 from wastewater using two different methods. This dataset is associated with the following publication: Ahmed, W., A. Bivins, A. Korajkic, S. Metcalfe, W.J.M. Smith, and S.L. Simpson. Comparative analysis of Adsorption-Extraction (AE) and Nanotrap® Magnetic Virus Particles (NMVP) workflows for the recovery of endogenous enveloped and non-enveloped viruses in wastewater. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 895(1): 160072, (2023).

This dataset contains the raw droplet counts resulting from of ddPCR or RT-ddPCR of nucleic acid extracts from wastewater. The wastewater was collected from three different sewersheds in Southwest Ohio (Mill Creek WWTP, Taylor Creek WWTP, and a sub-sewershed, Lick Run). ddPCR counts (positive droplets and total droplets) are provided for the following targets: N1 and N2 (SARS-CoV-2 nucleocapsid genes), crAssphage, PMMoV, HF183 (all fecal indicators), and OC43 (an RNA spike-in from a cultured coronavirus). Other metadata (pH, flow, temperature, TSS, CBOD5) are provided where available. This dataset is associated with the following publication: Nagarkar, M., S. Keely, M. Jahne, E. Wheaton, C. Hart, B. Smith, J. Garland, E. Varughese, A. Braam, B. Wiechman, B. Morris, and N. Brinkman. SARS-CoV-2 Monitoring at three sewersheds of different scales and complexity demonstrates distinctive relationships between wastewater measurements and COVID-19 case data. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 816: 151534, (2022).

Data described concentrations of human adenovirus, crAssphage and Pepper Mild Mottle virus in 1 hour composite wastewater samples and 24 h composite wastewater samples. This dataset is not publicly accessible because: Data is the property of CSIRO. It can be accessed through the following means: Contact Warish Ahmed, Warish.Ahmed@csiro.au. Format: Data is in excel format. This dataset is associated with the following publication: Ahmed, W., A. Bivins, P.M. Bertsch, K. Bibby, P. Gyawali, S.P. Sherchan, S.L. Simpson, K.V. Thomas, R. Verhagen, M. Kitajima, J.F. Mueller, and A. Korajkic. Intraday variability of indicator and pathogenic viruses in 1-h and 24-h composite wastewater samples: Implications for wastewater-based epidemiology. ENVIRONMENTAL RESEARCH. Elsevier B.V., Amsterdam, NETHERLANDS, 193: 110531, (2021).

Data describes percent losses of SARS-CoV-2 from wastewater. Also details recovery of endogenous SARS-CoV-2 from wastewater using two volumetrically different methods. This dataset is associated with the following publication: McMinn, B., A. Korajkic, A. Pemberton, J. Kelleher, W. Ahmed, and E. Villegas. Assessment of two volumetrically different concentration approaches to improve sensitivities for SARS-CoV-2 detection during wastewater monitoring. JOURNAL OF VIROLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 311: 114645, (2023).

Percent recovery data for SARS-CoV-2, concentration data for enteroviruses, adenoviruses and wastewater associated (Lachno3) marker genes. This dataset is not publicly accessible because: Data is not the property of EPA. It can be accessed through the following means: Contact korajkic.asja@epa.gov. Format: Data is the property of the University of Notre Dame. This dataset is associated with the following publication: Ahmed, W., A. Bivins, S.L. Simpson, W.J.M. Smith, S. Metcalfe, B. McMinn, E.M. Symonds, and A. Korajkic. Comparative Analysis of Rapid Concentration Methods for the Recovery of SARS-CoV-2 and Quantification of Human Enteric Viruses and Sewage-associated Lachno3 Marker Genes in Untreated Wastewater. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 799: 149386, (2021).

This data includes the results of an interlab methods comparison to recover and quantify SARS-CoV-2 from wastewater. This dataset is associated with the following publication: Pecson, B., E. Darby, C. Haas, Y. Amha, M. Bartolo, R. Danielson, Y. Dearborn, G. DiGiovanni, C. Ferguson, S. Fevig, E. Gaddis, D. Gray, G. Lukasik, B. Mull, L. Olivas, A. Olivieri, Y. Qu, and S. CoV-2. Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S.. Environmental Science: Water Research & Technology. Royal Society of Chemistry, Cambridge, UK, 7(3): 504-520, (2021).