The purpose of is this study was to evaluate the potential for biotransformation in the gastrointestinal tissues (GIT) of fish to impact chemical bioaccumulation. In vitro biotransformation of two polycyclic aromatic hydrocarbons, pyrene (PYR) and benzo[a]pyrene (BAP), and two organic sunscreen agents, 2-ethylhexyl-4-methoxycinnamate (EHMC) and octocrylene (OCT), was measured using S9 fractions isolated from liver tissue and tissues of the upper GIT in rainbow trout. For PYR, BAP, and EHMC, activity was substantially higher in liver S9 fractions than in GIT S9 fractions. For OCT, activity was highest in GIT S9 fractions. An existing in vitro-in vivo extrapolation (IVIVE) model for fish, which yields a whole-animal biotransformation rate constant (kMET), was expanded to consider biotransformation in the GIT. The kMET values obtained using measured rates of in vitro activity (liver and GIT) were in good agreement with kMET values measured in controlled in vivo experiments, providing strong support for the IVIVE approach. Moreover, inclusion of GIT activity into the model prediction for OCT resulted in much better agreement with the empirical kMET estimate than was obtained using a ‘liver only’ model. These findings suggest that current ‘liver only’ approaches to IVIVE modeling may underestimate in vivo whole-animal biotransformation rates for chemicals that undergo substantial biotransformation in the GIT. Thus, failure to consider biotransformation in the GIT may lead to overestimation of true levels of bioaccumulation. This dataset is associated with the following publication: Saunders, L., P. Fitzsimmons, J. Nichols, and F. Gobas. In vitro-in vivo extrapolation of hepatic and gastrointestinal biotrasnformation rates of hydrophobic chemicals in rainbow trout. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 228: 1-12, (2020).

,Zbiór zawiera wyniki kompleksowych testów laboratoryjnych komór laminarnych BioTectum DELTA (wersje: 1.2, 1.5 i 1.8) przeprowadzonych w warunkach badawczych przez Uniwersytet Przyrodniczo-Humanistyczny w Siedlcach. Zakres badań obejmuje ocenę konstrukcji, ergonomii, funkcjonalności, skuteczności filtracji powietrza, działania lamp UV oraz przydatności komór do prac mikrobiologicznych, molekularnych i kulturowania roślin in vitro. Celem testów było określenie przydatności komór do prowadzenia badań w warunkach sterylnych.,Dane obejmują m.in.:,

This Excel spreadsheet provides data that appear in Tables 1- 2 and Figures 1-4 of the main document, as well as data that appear in Figures S1-S3 of the Supplementary Information. This dataset is associated with the following publication: Nichols, J., M. Ladd, A. Hoffman, and P. Fitzsimmons. Biotransformation of polycyclic aromatic hydrocarbons by trout liver S9 fractions: Evaluation of competitive inhibition using a substrate depletion approach. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 38(12): 2729-2739, (2019).

,Zbiór zawiera wyniki testów funkcjonalnych oraz mikrobiologicznych komór laminarnych serii BioTectum DELTA (modele 1.2, 1.5, 1.8), przeprowadzonych w warunkach laboratoryjnych na Wydziale Nauk Biologicznych i Weterynaryjnych Uniwersytetu Mikołaja Kopernika w Toruniu. Celem badań było potwierdzenie skuteczności urządzeń w zapewnianiu środowiska sterylnego oraz ich przydatności do prac biologicznych, weterynaryjnych i biotechnologicznych.,Zakres testów obejmuje:,Raport dokumentuje metodykę badań, analizę wyników, dokumentację fotograficzną i wnioski końcowe,

Data corresponding to the figures in the paper. This dataset is associated with the following publication: Xing, Y., A. Ellis, M. Magnuson, and W. Harper. Adsorption of bacteriophage MS2 to colloids: Kinetics and particle interactions. Colloids and Surfaces A: Physicochemical and Engineering Aspects. Elsevier B.V., Amsterdam, NETHERLANDS, 585: 124099, (2020).

These data are bacterial 16S rRNA sequences and a taxonomic summary table for biofilm samples from the bio-reactors. The data may provide background/supporting information for other researchers who have a similar experimental plan with a microbial electrochemical cell reactor. This dataset is associated with the following publication: Santodomingo, J., H. Lee, B. Dhar, J. An, B. Rittmann, H. Ren, and J. Chae. The Roles of Biofilm Conductivity and Donor Substrate Kinetics in a Mixed-Culture Biofilm Anod. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 50(23): 12799-12807, (2016).

Understanding biotransformation pathways in aquatic species is an integral part of ecological risk assessment with respect to the potential bioactivation of chemicals to more toxic metabolites. The long-range goal is to gain sufficient understanding of fish metabolic transformation reactions to be able to accurately predict fish xenobiotic metabolism. While some metabolism data exist, there are few fish in vivo exposure studies where metabolites have been identified and the metabolic pathways proposed. Previous biotransformation work has focused on in vitro studies which have the advantage of high throughput but may have limited metabolic capabilities, and in vivo studies which have full metabolic capacity but are low throughput. An aquatic model system with full metabolic capacity in which a large number of chemicals could be tested would be a valuable tool. The current study evaluated the ex vivo rainbow trout liver slice model, which has the advantages of high throughput as found in vitro models and non-dedifferentiated cells and cell to cell communication found in in vivo systems. The pesticide diazinon, which has been previously tested both in vitro and in vivo in a number of mammalian and aquatic species including rainbow trout, was used to evaluate the ex vivo slice model as a tool to study biotransformation pathways. While somewhat limited by the analytical chemistry method employed, results of the liver slice model, mainly that hydroxypyrimidine was the major diazinon metabolite, are in line with the results of previous rainbow trout in vivo studies. Therefore, the rainbow trout liver slice model is a useful tool for the study of metabolism in aquatic species. This dataset is associated with the following publication: Tapper, M., J. Serrano, P. Schmieder, D. Hammermeister, and R. Kolanczyk. Metabolism of diazinon in rainbow trout liver slices. Applied In Vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 4(1): 13-23, (2018).

The dataset, which is presented as an Excel spreadsheet, contains all data which is presented as figures in Nichols et al., Measurement of kinetic parameters for biotransformation of polycyclic aromatic hydrocarbons by trout liver S9 fractions: Implications for bioaccumulation assessment, accepted for publication in Applied In Vitro Toxicology 04/2017. Additional information if provided regarding reaction conditions used to characterize liver S9 fractions and perform PAH depletions studies. This dataset is associated with the following publication: Nichols, J., M. Ladd, and P. Fitzsimmons. Measurement of kinetic parameters for biotransformation of polycyclic aromatic hydrocarbons by trout liver S9 fractions: Implications for bioaccumulation assessment. Applied In Vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 4(4): 365-378, (2018).