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SciHub Data for Haugland et al JMM 2020
Paired data for estimates of E. coli concentrations by culture analysis and E. coli gene copies by Draft Method C for 6965 ambient water samples from state of Michigan. This dataset is associated with the following publication: Haugland, R., K. Oshima, and M. Sivaganesan. Large-scale comparison of E. coli levels determined by culture and a qPCR method (EPA Draft Method C) in Michigan towards the implementation of rapid, multi-site beach testing. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 184: 106186, (2021).
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Identify sources of high E. coli concentrations, beaches of southern Lake Michigan, 2015, (version 2.0, July 2020)
공공데이터포털
Data were collected as part of a study to identify sources of E. coli contamination at several beaches located in the Grand Calumet River Areas of Concern, located in northern Indiana on Lake Michigan, as well as in Illinois and Wisconsin on Lake Michigan. Water samples were collected at each site in Indiana three times a week for thirteen weeks and at each site in Illinois and Wisconsin. All samples were analyzed for E. coli bacteria (an indicator bacteria for fecal contamination) and species-specific molecular markers (microbial source tracking, MST), including human, gull, and dog. Presence of MST markers indicates a fecal source at that location associated with the target animal. Field conditions were recorded during each site visit (e.g., including air and water temp, wind speed and direction, rainfall, wave height, currents, observations). Water samples were analyzed in the laboratory for E. coli using defined substrate technology and for MST markers using quantitative polymerase chain reaction (qPCR) methods and also turbidity. Lat_long_v2 file includes information regarding sampling locations and their corresponding latitude and longitudes. The E coli_turbidity_v2 data include E. coli densities and turbidity measurements. Data from the qpcr_v4 file includes results from quantitative polymerase chain reaction (qPCR) method for detection of host-specific microbial source tracking markers. Data from SanitarySurvey includes ambient conditions measured in the field: water and air temperature, current speed and direction (eastward, westward, float method), wind direction and speed, wave height, rainfall, and cloud cover. Data from SanitarySurvey_birds included number of birds (gulls, geese, ducks, cormorants, etc.) counted on the beach and in the water. The PowerWater.pdf is the DNA extraction kits' user manual giving detailed instructions for use.
Identify sources of high E. coli concentrations, beaches of southern Lake Michigan, 2015, (version 2.0, July 2020)
공공데이터포털
Data were collected as part of a study to identify sources of E. coli contamination at several beaches located in the Grand Calumet River Areas of Concern, located in northern Indiana on Lake Michigan, as well as in Illinois and Wisconsin on Lake Michigan. Water samples were collected at each site in Indiana three times a week for thirteen weeks and at each site in Illinois and Wisconsin. All samples were analyzed for E. coli bacteria (an indicator bacteria for fecal contamination) and species-specific molecular markers (microbial source tracking, MST), including human, gull, and dog. Presence of MST markers indicates a fecal source at that location associated with the target animal. Field conditions were recorded during each site visit (e.g., including air and water temp, wind speed and direction, rainfall, wave height, currents, observations). Water samples were analyzed in the laboratory for E. coli using defined substrate technology and for MST markers using quantitative polymerase chain reaction (qPCR) methods and also turbidity. Lat_long_v2 file includes information regarding sampling locations and their corresponding latitude and longitudes. The E coli_turbidity_v2 data include E. coli densities and turbidity measurements. Data from the qpcr_v4 file includes results from quantitative polymerase chain reaction (qPCR) method for detection of host-specific microbial source tracking markers. Data from SanitarySurvey includes ambient conditions measured in the field: water and air temperature, current speed and direction (eastward, westward, float method), wind direction and speed, wave height, rainfall, and cloud cover. Data from SanitarySurvey_birds included number of birds (gulls, geese, ducks, cormorants, etc.) counted on the beach and in the water. The PowerWater.pdf is the DNA extraction kits' user manual giving detailed instructions for use.
Coalinga Canal E. coli MPN/100mL Time Series Data
공공데이터포털
Measurements of E. coli collected at Coalinga Canal. Currently collected twice a year, previously collected quarterly. Access further information for this data set by contacting Bureau of Reclamation, California-Great Basin Region, Environmental Affairs Division (CGB-157). See ResultAttributes for STAFF_GAUGE, SMPL_DEPTH, SMPL_CATEGORY_NAME, METHOD_CODE, RESULT_RL, RESULT_RL-UNIT_STD_NAME, RESULT_MDL, RESULT_MDL-UNIT_STD_NAME, USBR_QA_SUBTYPE_NAME, USBR_QULFR_DESCRIPTION. STAFF_GAUGE is the water height in decimal feet measured by gauge (e.g., 15.2). SMPL_DEPTH is the vertical depth at which sample is collected (e.g., 0 - 15 cm). For water samples: depth below water/air interface. For sediment and soil samples: depth below water/solid or air/solid interface. SMPL_CATEGORY_NAME is the category type of sample (e.g., Composite). METHOD_CODE is the name of method used to obtain result (e.g., EPA 200.8). RESULT_RL is the result reporting limit (accounting for dilution) (e.g., 0.02). RESULT_RL-UNIT_STD_NAME is the unit associated with RESULT_RL (e.g., mg/L). RESULT_MDL is the result method detection limit (e.g., 0.007). RESULT_MDL-UNIT_STD_NAME is the unit associated with RESULT_MDL (e.g., mg/L). USBR_QA_SUBTYPE_NAME is the quality control type of the sample (e.g., USBR_BLANK_SPIKE). USBR_QULFR_DESCRIPTION is the quality assurance description (if any) (e.g., Result may have a high bias.).
E. coli dataset at from the Outfall of the Nogales International Wastewater Treatment Plant, geographic NAD83 (Water Years 2009–2015)
공공데이터포털
This dataset contains E. coli data collected at the Outfall of the Nogales International Wastewater Treatment Plant (NIWTP; SC6, 312724110580501) by U.S. International Boundary and Water Commission (IBWC) personnel. The IBWC collects discrete samples at the Outfall of the NIWTP daily or weekly by dipping a 100 mL sterilized IDEXX bottle in the centroid of flow. Samples are kept chilled and shipped to Turner Laboratories Inc. or Legend Technical Services Inc. Both laboratories use standard method 9223B for E. coli analysis, which uses the Colilert™-24 (Colilert) and Quanti-Tray®/2000 (Quanti-Tray) system. The holding time specified for wastewater is 30 hours. The dataset is a compilation of multiple paper records. E.coli concentrations reported as 0 most probable number (MPN) per100 mL were qualified as less than the 1 MPN/100mL specified by the Colilert test. The datasest includes E. coli concentration data for the period of June 01, 2009 to March 31, 2015.
E. coli dataset at from the Outfall of the Nogales International Wastewater Treatment Plant, geographic NAD83 (Water Years 2009–2015)
공공데이터포털
This dataset contains E. coli data collected at the Outfall of the Nogales International Wastewater Treatment Plant (NIWTP; SC6, 312724110580501) by U.S. International Boundary and Water Commission (IBWC) personnel. The IBWC collects discrete samples at the Outfall of the NIWTP daily or weekly by dipping a 100 mL sterilized IDEXX bottle in the centroid of flow. Samples are kept chilled and shipped to Turner Laboratories Inc. or Legend Technical Services Inc. Both laboratories use standard method 9223B for E. coli analysis, which uses the Colilert™-24 (Colilert) and Quanti-Tray®/2000 (Quanti-Tray) system. The holding time specified for wastewater is 30 hours. The dataset is a compilation of multiple paper records. E.coli concentrations reported as 0 most probable number (MPN) per100 mL were qualified as less than the 1 MPN/100mL specified by the Colilert test. The datasest includes E. coli concentration data for the period of June 01, 2009 to March 31, 2015.
Klamath Straits Drain At Hwy 161 E. coli MPN/100mL Time Series Data
공공데이터포털
Measurements of E. coli collected at Klamath Straits Drain At Hwy 161. Currently collected twice a year, previously collected quarterly. Access further information for this data set by contacting Bureau of Reclamation, California-Great Basin Region, Environmental Affairs Division (CGB-157). See ResultAttributes for STAFF_GAUGE, SMPL_DEPTH, SMPL_CATEGORY_NAME, METHOD_CODE, RESULT_RL, RESULT_RL-UNIT_STD_NAME, RESULT_MDL, RESULT_MDL-UNIT_STD_NAME, USBR_QA_SUBTYPE_NAME, USBR_QULFR_DESCRIPTION. STAFF_GAUGE is the water height in decimal feet measured by gauge (e.g., 15.2). SMPL_DEPTH is the vertical depth at which sample is collected (e.g., 0 - 15 cm). For water samples: depth below water/air interface. For sediment and soil samples: depth below water/solid or air/solid interface. SMPL_CATEGORY_NAME is the category type of sample (e.g., Composite). METHOD_CODE is the name of method used to obtain result (e.g., EPA 200.8). RESULT_RL is the result reporting limit (accounting for dilution) (e.g., 0.02). RESULT_RL-UNIT_STD_NAME is the unit associated with RESULT_RL (e.g., mg/L). RESULT_MDL is the result method detection limit (e.g., 0.007). RESULT_MDL-UNIT_STD_NAME is the unit associated with RESULT_MDL (e.g., mg/L). USBR_QA_SUBTYPE_NAME is the quality control type of the sample (e.g., USBR_BLANK_SPIKE). USBR_QULFR_DESCRIPTION is the quality assurance description (if any) (e.g., Result may have a high bias.).
Folsom Lake On Dam Face E. coli MPN/100mL Time Series Data
공공데이터포털
Measurements of E. coli collected at Folsom Lake On Dam Face. Currently collected twice a year, previously collected quarterly. Access further information for this data set by contacting Bureau of Reclamation, California-Great Basin Region, Environmental Affairs Division (CGB-157). See ResultAttributes for STAFF_GAUGE, SMPL_DEPTH, SMPL_CATEGORY_NAME, METHOD_CODE, RESULT_RL, RESULT_RL-UNIT_STD_NAME, RESULT_MDL, RESULT_MDL-UNIT_STD_NAME, USBR_QA_SUBTYPE_NAME, USBR_QULFR_DESCRIPTION. STAFF_GAUGE is the water height in decimal feet measured by gauge (e.g., 15.2). SMPL_DEPTH is the vertical depth at which sample is collected (e.g., 0 - 15 cm). For water samples: depth below water/air interface. For sediment and soil samples: depth below water/solid or air/solid interface. SMPL_CATEGORY_NAME is the category type of sample (e.g., Composite). METHOD_CODE is the name of method used to obtain result (e.g., EPA 200.8). RESULT_RL is the result reporting limit (accounting for dilution) (e.g., 0.02). RESULT_RL-UNIT_STD_NAME is the unit associated with RESULT_RL (e.g., mg/L). RESULT_MDL is the result method detection limit (e.g., 0.007). RESULT_MDL-UNIT_STD_NAME is the unit associated with RESULT_MDL (e.g., mg/L). USBR_QA_SUBTYPE_NAME is the quality control type of the sample (e.g., USBR_BLANK_SPIKE). USBR_QULFR_DESCRIPTION is the quality assurance description (if any) (e.g., Result may have a high bias.).
Santa Ynez River Below Lake Cachuma E. coli MPN/100mL Time Series Data
공공데이터포털
Measurements of E. coli collected at Santa Ynez River Below Lake Cachuma. Currently collected twice a year, previously collected quarterly. Access further information for this data set by contacting Bureau of Reclamation, California-Great Basin Region, Environmental Affairs Division (CGB-157). See ResultAttributes for STAFF_GAUGE, SMPL_DEPTH, SMPL_CATEGORY_NAME, METHOD_CODE, RESULT_RL, RESULT_RL-UNIT_STD_NAME, RESULT_MDL, RESULT_MDL-UNIT_STD_NAME, USBR_QA_SUBTYPE_NAME, USBR_QULFR_DESCRIPTION. STAFF_GAUGE is the water height in decimal feet measured by gauge (e.g., 15.2). SMPL_DEPTH is the vertical depth at which sample is collected (e.g., 0 - 15 cm). For water samples: depth below water/air interface. For sediment and soil samples: depth below water/solid or air/solid interface. SMPL_CATEGORY_NAME is the category type of sample (e.g., Composite). METHOD_CODE is the name of method used to obtain result (e.g., EPA 200.8). RESULT_RL is the result reporting limit (accounting for dilution) (e.g., 0.02). RESULT_RL-UNIT_STD_NAME is the unit associated with RESULT_RL (e.g., mg/L). RESULT_MDL is the result method detection limit (e.g., 0.007). RESULT_MDL-UNIT_STD_NAME is the unit associated with RESULT_MDL (e.g., mg/L). USBR_QA_SUBTYPE_NAME is the quality control type of the sample (e.g., USBR_BLANK_SPIKE). USBR_QULFR_DESCRIPTION is the quality assurance description (if any) (e.g., Result may have a high bias.).
Identify sources of high E. coli concentrations, Grand Calumet River Area of Concern beaches of southern Lake Michigan, 2016-2018
공공데이터포털
Data were collected as part of a study to identify sources of E. coli contamination at several beaches located in the Grand Calumet River Areas of Concern, located in northern Indiana on Lake Michigan. The study was funded by the Great Lakes Restoration Initiative. Water samples were collected at each site (Jeorse Park 1, Jeorse Park 2, Hammond East, Hammond West, Whihala West, Whihala East, Whihala west breakwater, Hammond Marina, Whihala offshore locations, and the Grand Calumet River) one day a week or three times a week between 2015 and 2018. While the 2015 data were included in analysis, these data were previously publicly released https://doi.org/10.5066/F7H70F3D. Samples (water, sand, sediment) were analyzed for E. coli bacteria (an indicator bacterium for fecal contamination) and species-specific molecular markers (microbial source tracking, MST), including human (HF183, Mnif), gull (Gull2), and dog (DogBact). Presence of MST markers indicates a fecal source associated with the target animal at that location. Bird counts were recorded during each site visit. Water samples were analyzed in the laboratory for E. coli using defined substrate technology, for MST markers using quantitative polymerase chain reaction (qPCR) methods, and turbidity, conductivity, and pH. Additionally in 2015, samples were collected during two dry and one wet event and subjected to metagenomics analysis for determining bacterial communities. The additional samples were collected at Whihala, Jeorse Park, Grand Calumet River, as well as three offshore locations associated with the Grand Calumet River: mouth to the lake at a peninsular structure and north and east of the structure. Lat_long file includes information regarding sampling locations and their corresponding latitude and longitudes. The EcoliWaterChem data include E. coli densities and water chemistry (turbidity, conductivity, pH) measurements. Data from the MST file includes results from quantitative polymerase chain reaction (qPCR) method for detection of host-specific microbial source tracking markers as follows: Gulls (Gull2), Dogs (DogBact), and human (HF183, Mnif). Data from Birds included number of birds (gulls, geese, ducks, cormorants, etc.) counted on the beach and in the water. The metagenomics file includes taxonomic information and corresponding percent abundance of each organism for each sample. The metagenomics file includes taxonomic information and corresponding organisms' percent abundance for each sample. The metagenomicsKey file includes information pertinent to individual samples.
Identify sources of high E. coli concentrations, Grand Calumet River Area of Concern beaches of southern Lake Michigan, 2016-2018
공공데이터포털
Data were collected as part of a study to identify sources of E. coli contamination at several beaches located in the Grand Calumet River Areas of Concern, located in northern Indiana on Lake Michigan. The study was funded by the Great Lakes Restoration Initiative. Water samples were collected at each site (Jeorse Park 1, Jeorse Park 2, Hammond East, Hammond West, Whihala West, Whihala East, Whihala west breakwater, Hammond Marina, Whihala offshore locations, and the Grand Calumet River) one day a week or three times a week between 2015 and 2018. While the 2015 data were included in analysis, these data were previously publicly released https://doi.org/10.5066/F7H70F3D. Samples (water, sand, sediment) were analyzed for E. coli bacteria (an indicator bacterium for fecal contamination) and species-specific molecular markers (microbial source tracking, MST), including human (HF183, Mnif), gull (Gull2), and dog (DogBact). Presence of MST markers indicates a fecal source associated with the target animal at that location. Bird counts were recorded during each site visit. Water samples were analyzed in the laboratory for E. coli using defined substrate technology, for MST markers using quantitative polymerase chain reaction (qPCR) methods, and turbidity, conductivity, and pH. Additionally in 2015, samples were collected during two dry and one wet event and subjected to metagenomics analysis for determining bacterial communities. The additional samples were collected at Whihala, Jeorse Park, Grand Calumet River, as well as three offshore locations associated with the Grand Calumet River: mouth to the lake at a peninsular structure and north and east of the structure. Lat_long file includes information regarding sampling locations and their corresponding latitude and longitudes. The EcoliWaterChem data include E. coli densities and water chemistry (turbidity, conductivity, pH) measurements. Data from the MST file includes results from quantitative polymerase chain reaction (qPCR) method for detection of host-specific microbial source tracking markers as follows: Gulls (Gull2), Dogs (DogBact), and human (HF183, Mnif). Data from Birds included number of birds (gulls, geese, ducks, cormorants, etc.) counted on the beach and in the water. The metagenomics file includes taxonomic information and corresponding percent abundance of each organism for each sample. The metagenomics file includes taxonomic information and corresponding organisms' percent abundance for each sample. The metagenomicsKey file includes information pertinent to individual samples.