The data set is six separate csv files. Four of which contain the quantity of DNA copy numbers and fluor used to analyze the DNA quantities collected from water samples from four separate portions of the study (adult SL field, adult SL lab, larval SL field, larval SL lab) and need to be in their own csv file. Also included is a csv with adult SL trapping data, a csv for larval SL shocking data, and a csv with the volume that was filtered for our DNA extractions.

These data contain biodata, net catch, and detection records for juvenile sea lamprey sampled in natural streams in Michigan and Quebec, Canada and those stocked into an artificial stream at the USGS Hammond Bay Biological Station for monitoring diel activity. During October 31 through November 9, 2011 scientists collected downstream migrating juvenile sea lamprey from the Little Carp River, Michigan, Lake Superior (46°50'6.34"N 88°28'57.58"W). Collections were permitted by the State of Michigan under the Michigan Department of Natural Resources Scientific Collectors Permit issued to U.S. Geological Survey, Hammond Bay Biological Station December 12, 2007; amended February 23, 2011. Between November 3, 2014 and December 15, 2014, scientists monitored lamprey downstream passage using passive integrated transponder (PIT) telemetry in Morpion Stream, Quebec, Canada, Lake Champlain (45°10'23.68"N 73° 2'16.98"W). During October 2014 through February 2015, scientists monitored movement activity of juvenile sea lamprey using PIT telemetry in an artificial stream located at the Hammond Bay Biological Station, Millersburg, Michigan. Collections from Morpion Stream, Quebec during 2014 were conducted under Quebec Ministere des Resources Naturelles permis a des fins scientifiques 2012-10-01-1436-16-SP to Ellen Marsden and a scientific collection permit from the Vermont Department of Fish and Wildlife. Fish care and protocols for fish holding, surgery, and tagging were conducted under a University of Vermont Institutional Animal Care and Use Committee, permit 13-017.

qPCR detection results for Pacific Lamprey eDNA surveys from Western Washington Rivers and Streams. Water samples were collected via filtration and shipped to the laboratory for analysis. DNA was extracted from filters using commercially available extraction kits. Lab staff used two existing Pacific Lamprey qPCR eDNA surveys to screen DNA extracts for the presence of Pacific Lamprey DNA. Eight molecular replicates were run for each samples. Results presented in this table show the number of positive detections out of 8 for each sample and the corresponding mean Cq values for each sample with a detection.

qPCR detection results for Pacific Lamprey eDNA surveys from Western Washington Rivers and Streams. Water samples were collected via filtration and shipped to the laboratory for analysis. DNA was extracted from filters using commercially available extraction kits. Lab staff used two existing Pacific Lamprey qPCR eDNA surveys to screen DNA extracts for the presence of Pacific Lamprey DNA. Eight molecular replicates were run for each samples. Results presented in this table show the number of positive detections out of 8 for each sample and the corresponding mean Cq values for each sample with a detection.

This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis