Dataset containing experimental data and R scripts used for data analyses. This dataset is associated with the following publication: Van Meter, R., D. Glinski, M. Henderson , and T. Purucker. Soil organic matter content effects on dermal pesticide bioconcentration in American toads (Bufo americanus).. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 35(11): 2734–2741, (2016).

Dermal uptake from soil is known to occur in amphibians, but predicting pesticide availability and bioconcentration across soil types is not well understood. The present study was designed to compare uptake of 5 current-use pesticides (imidacloprid, atrazine, triadimefon, fipronil, and pendimethalin) in American toads (Bufo americanus) from exposure on soils with significant organic matter content differences (14.1% = high organic matter and 3.1% = low organic matter). We placed toads on high- or low-organic matter soil after applying individual current-use pesticides on the soil surface for an 8-h exposure duration. Whole body tissue homogenates and soils were extracted and analyzed using liquid chromatography–mass spectrometry to determine pesticide tissue and soil concentration, as well as bioconcentration factor in toads. Tissue concentrations were greater on the low-organic matter soil than the high-organic matter soil across all pesticides (average ± standard error; 1.23 ± 0.35 ppm and 0.78 ± 0.23 ppm, respectively), and bioconcentration was significantly higher for toads on the low-organic matter soil (analysis of covariance p = 0.002). Soil organic matter is known to play a significant role in the mobility of pesticides and bioavailability to living organisms. Agricultural soils typically have relatively lower organic matter content and serve as a functional habitat for amphibians. The potential for pesticide accumulation in amphibians moving throughout agricultural landscapes may be greater and should be considered in conservation and policy efforts. This dataset is associated with the following publication: VanMeter, R., D. Glinski, T. Hong, M. Cyterski , M. Henderson , and T. Purucker. Estimating terrestrial amphibian pesticide body burden through dermal exposure. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 193: 262-268, (2014). NOTE: This dataset has been removed from public access due to revocation. Please refer inquiries regarding this dataset to the listed contact person.

Dermal uptake from soil is known to occur in amphibians, but predicting pesticide availability and bioconcentration across soil types is not well understood. The present study was designed to compare uptake of 5 current-use pesticides (imidacloprid, atrazine, triadimefon, fipronil, and pendimethalin) in American toads (Bufo americanus) from exposure on soils with significant organic matter content differences (14.1% = high organic matter and 3.1% = low organic matter). We placed toads on high- or low-organic matter soil after applying individual current-use pesticides on the soil surface for an 8-h exposure duration. Whole body tissue homogenates and soils were extracted and analyzed using liquid chromatography–mass spectrometry to determine pesticide tissue and soil concentration, as well as bioconcentration factor in toads. Tissue concentrations were greater on the low-organic matter soil than the high-organic matter soil across all pesticides (average ± standard error; 1.23 ± 0.35 ppm and 0.78 ± 0.23 ppm, respectively), and bioconcentration was significantly higher for toads on the low-organic matter soil (analysis of covariance p = 0.002). Soil organic matter is known to play a significant role in the mobility of pesticides and bioavailability to living organisms. Agricultural soils typically have relatively lower organic matter content and serve as a functional habitat for amphibians. The potential for pesticide accumulation in amphibians moving throughout agricultural landscapes may be greater and should be considered in conservation and policy efforts. This dataset is associated with the following publication: VanMeter, R., D. Glinski, T. Hong, M. Cyterski , M. Henderson , and T. Purucker. Estimating terrestrial amphibian pesticide body burden through dermal exposure. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 193: 262-268, (2014). NOTE: This dataset has been removed from public access due to revocation. Please refer inquiries regarding this dataset to the listed contact person.

Scripts and datasets used to merge available amphibian exposure into a collated data set with data analyses. This dataset is associated with the following publication: Purucker, T., M. Snyder, D. Glinski, R. Van Meter, K. Garber, E. Chelsvig, M. Cyterski, S. Sinnathamby, E. Paulukonis, and M. Henderson. Estimating dermal contact soil exposure for amphibians. Integrated Environmental Assessment and Management. Allen Press, Inc., Lawrence, KS, USA, 19(1): 9-16, (2022).

Scripts and datasets used to merge available amphibian exposure into a collated data set with data analyses. This dataset is associated with the following publication: Purucker, T., M. Snyder, D. Glinski, R. Van Meter, K. Garber, E. Chelsvig, M. Cyterski, S. Sinnathamby, E. Paulukonis, and M. Henderson. Estimating dermal contact soil exposure for amphibians. Integrated Environmental Assessment and Management. Allen Press, Inc., Lawrence, KS, USA, 19(1): 9-16, (2022).

GC/MS data resulting from the metabolomic analysis of amphibian livers following exposure to common agrochemicals (fertilizer and pesticides) and their mixtures. This dataset is associated with the following publication: Van Meter, R.J., D. Glinski, T. Purucker, and M. Henderson. Induced Hepatic Glutathione and Metabolomic Alterations Following Mixed Pesticide and Fertilizer Exposures in Juvenile Leopard Frogs (Lithobates sphenocephala). ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 41(1): 122-133, (2022).

GC/MS data resulting from the metabolomic analysis of amphibian livers following exposure to common agrochemicals (fertilizer and pesticides) and their mixtures. This dataset is associated with the following publication: Van Meter, R.J., D. Glinski, T. Purucker, and M. Henderson. Induced Hepatic Glutathione and Metabolomic Alterations Following Mixed Pesticide and Fertilizer Exposures in Juvenile Leopard Frogs (Lithobates sphenocephala). ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 41(1): 122-133, (2022).

GC/MS and LC/MS-MS analysis of exposure media and concentrations in amphibians following exposure studies. This dataset is associated with the following publication: Glinski, D., R.J. Van Meter, T. Purucker, and M. Henderson. Route of exposure influences pesticide body burden and the hepatic metabolome in post-metamorphic leopard frogs. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 779: 146358, (2021).

GC/MS and LC/MS-MS analysis of exposure media and concentrations in amphibians following exposure studies. This dataset is associated with the following publication: Glinski, D., R.J. Van Meter, T. Purucker, and M. Henderson. Route of exposure influences pesticide body burden and the hepatic metabolome in post-metamorphic leopard frogs. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 779: 146358, (2021).

Amphibian metabolite data used in Snyder, M.N., Henderson, W.M., Glinski, D.G., Purucker, S. T., 2017. Biomarker analysis of american toad (Anaxyrus americanus) and grey tree frog (Hyla versicolor) tadpoles following exposure to atrazine. Aquatic Toxicology, 182(184-193). doi: 10.1016/j.aquatox.2016.11.018. This dataset is associated with the following publication: Snyder, M., M. Henderson, D. Glinski, and T. Purucker. Biomarker analysis of American toad (Anaxyrus americanus) and grey tree frog (Hyla versicolor) tadpoles following exposure to atrazine.. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 182: 184-193, (2017).