The prevalence of antibiotic resistance genes in microbial communities from sewage wastewater streams and from offshore marine sediments in the vicinity of sewage wastewater outfalls in Southeast Florida was investigated from June 2018 to March 2019. Sediment and wastewater samples were analyzed for 15 different antibiotic resistant gene targets via polymerase chain reaction (PCR) presence/absence assays in Southeast Florida coral reef environments. Data collected from five sites (Broward North Wastewater Treatment Plant (WWTP), Broward North WWTP Outfall, Haµlover (Miami-Dade North) Outfall, Hollywood Outfall, Hollywood WWTP, and Miami-Dade North WWTP) illustrated widespread prevalence of antibiotic resistance genes in these microbial communities with the highest concentrations occurring in the sewage wastewater stream and in close proximity to the outfall pipe and outfall. Data indicated seasonal (wet versus dry season) trends and potential public and ecosystem health risks. Additionally, a reef in the Florida Keys was evaluated during the wet season using the same tools and approach prior to and after diseased corals were treated with amoxicillin. Resistance to amoxicillin was only observed in the post-treatment sample set.

Crocker Reef was the site of an integrated reefscape characterization effort focused on calcification and related biogeochemical processes as part of the USGS Coral Reef Ecosystem Study (CREST) project. This effort included two intensive seasonal sampling trips to capture summer (July 8 to 17, 2014) and winter (January 29 to February 5, 2015) conditions. This data release represents water column microbial and environmental data collected for use as metadata in future publications examining reef metabolic processes via metagenomes derived from water samples and fine-scale temporal and spatial carbonate chemistry measurements. Microbial data are total bacterial counts per milliliter seawater, total viral counts per milliliter of seawater, and plate counts using Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar of Vibrio (bacteria) per milliliter of seawater. Environmental data are water temperature, salinity, dissolved oxygen, and pH.

Crocker Reef was the site of an integrated reefscape characterization effort focused on calcification and related biogeochemical processes as part of the USGS Coral Reef Ecosystem Study (CREST) project. This effort included two intensive seasonal sampling trips to capture summer (July 8 to 17, 2014) and winter (January 29 to February 5, 2015) conditions. This data release represents water column microbial and environmental data collected for use as metadata in future publications examining reef metabolic processes via metagenomes derived from water samples and fine-scale temporal and spatial carbonate chemistry measurements. Microbial data are total bacterial counts per milliliter seawater, total viral counts per milliliter of seawater, and plate counts using Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar of Vibrio (bacteria) per milliliter of seawater. Environmental data are water temperature, salinity, dissolved oxygen, and pH.

Isolates listed by unique identifiers and levels of resistance to various antibiotics. This dataset is associated with the following publication: Hoelle, J., J.R. Johnson, B. Johnston, B. Kinkle, L. Boczek, H. Ryu, and S. Hayes. Survey of US wastewater for carbapenem-resistant Enterobacteriaceae. JOURNAL OF WATER AND HEALTH. IWA Publishing, London, UK, 17(2): 219-226, (2019).

Isolates listed by unique identifiers and levels of resistance to various antibiotics. This dataset is associated with the following publication: Hoelle, J., J.R. Johnson, B. Johnston, B. Kinkle, L. Boczek, H. Ryu, and S. Hayes. Survey of US wastewater for carbapenem-resistant Enterobacteriaceae. JOURNAL OF WATER AND HEALTH. IWA Publishing, London, UK, 17(2): 219-226, (2019).

In this study, the abundance and distribution of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs), as well as the concentrations of antibiotics present in a mixed-use watershed in Athens, GA, USA were examined, in order to enhance understanding of the existing state of AR in the freshwater environment. The current study has shown that antibiotic-related contaminants are prevalent in the freshwater environment, including commensal and pathogenic bacteria that are resistant to antibiotics used for human and veterinary purposes, medically important antibiotics, as well as the genes associated with resistance to these antibiotics. This dataset is not publicly accessible because: Data belong to coauthor at USDA ARS. It can be accessed through the following means: The data presented in this study are available on request from the corresponding author, Jonathan Frye at USDA. Format: Statistical analysis of data from surface water samples, see the journal article's Supplementary Materials for additional information: https://www.mdpi.com/article/10.3390/antibiotics12111586/s1. This dataset is associated with the following publication: Cho, S., L. Hiott, Q. Read, J. Damashek, J. Westrich, M. Edwards, R. Seim, D. Glinski, J. Bateman McDonald, E. Ottesen, E. Lipp, M. Henderson, C. Jackson, and J. Frye. Distribution of Antibiotic Resistance in a Mixed-Use Watershed and the Impact of Wastewater Treatment Plants on Antibiotic Resistance in Surface Water. The Journal of Antibiotics. Springer Nature, New York, NY, USA, 12(11): 1586, (2023).

Includes annual probabilities of colonization (Pcol), probabilities of skin and blood stream (bs) infection (Pinf), and associated disability adjusted life years (DALY) for exposure to methicillin-susceptible and -resistant Staphylococcus aureus (MSSA/MRSA) in reclaimed greywater (GW) and wastewater (WW) as described in the article's text. A data dictionary is included in the metadata tab of the workbook. This dataset is associated with the following publication: Schoen, M., M. Jahne, J. Garland, L. Ramirez, A. Lopatkin, and K. Hamilton. Quantitative microbial risk assessment of antimicrobial resistant and susceptible Staphylococcus aureus in reclaimed wastewaters. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 55(20): 13413-14330, (2021).

Includes annual probabilities of colonization (Pcol), probabilities of skin and blood stream (bs) infection (Pinf), and associated disability adjusted life years (DALY) for exposure to methicillin-susceptible and -resistant Staphylococcus aureus (MSSA/MRSA) in reclaimed greywater (GW) and wastewater (WW) as described in the article's text. A data dictionary is included in the metadata tab of the workbook. This dataset is associated with the following publication: Schoen, M., M. Jahne, J. Garland, L. Ramirez, A. Lopatkin, and K. Hamilton. Quantitative microbial risk assessment of antimicrobial resistant and susceptible Staphylococcus aureus in reclaimed wastewaters. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 55(20): 13413-14330, (2021).

This data set includes information on collections of surface water and fecal samples from wild gulls (Larus spp.) at two locations on the Kenai Peninsula, Alaska, USA. Samples were screened for Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) and tested for resistance to multiple antibiotics.

This data set includes information on collections of surface water and fecal samples from wild gulls (Larus spp.) at two locations on the Kenai Peninsula, Alaska, USA. Samples were screened for Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) and tested for resistance to multiple antibiotics.