As part of a larger study looking at the seasonal distribution and relative abundance of the southern house mosquito Culex quinquefasciatus in Kīpahulu Valley, Haleakalā National Park, adult and immature mosquito distributions and abundance was monitored at two altitudinal sites throughout the year. Study sites at Delta Camp (915 m asl) and Palikea Camp (1280 m asl) were visited twice during each three-month season from September 2022 to September 2023. At each study site, 15 BioGents (BG) Sentinel II traps (S), 15 BioGents Pro traps (P), 15 Center for Disease Control (CDC) Gravid traps (G), and 15 ovicups to monitor egg laying females (and collect egg rafts) were operated for 4-7 nights across a 1-kilometer square area. Concurrent with adults trapping, terrestrial and stream transects were surveyed for potential larval mosquito habitat and the presence and abundance of larval mosquitoes. This data file consists of spatial coordinates for all adult mosquito trap and larval mosquito site locations along terrestrial transects and streams, as well as environmental DNA (eDNA) water samples taken at both field sites (the results for which will be included in a future release).