These data contain the study site locations and elevation information where bolts (tree stem sections) infected with Ceratocystis lukuohia and/or C. huliohia were collected on Hawai'i Island.

This data release includes data and metadata containing (1) study site locations and elevation where bolts (tree stem sections) infected with Ceratocystis lukuohia and/or C. huliohia were collected on Hawai'i Island, (2) the ambrosia beetle (Coleoptera: Scolytinae) species that were reared from bolts, (3) Ceratocystis spp. viability results from culturing tests of the beetles' frass, (4) frass production estimates from three ambrosia beetle species, and (5) quantitative Polymerase Chain Reaction (qPCR) DNA results of viability subcultures. Data were used in the analysis for the manuscript "Ambrosia beetle communities and frass production in ʻōhiʻa (Metrosideros polymorpha) infected with Ceratocystis fungi responsible for Rapid ʻŌhiʻa Death." Beetles and frass were collected in the lab from Rapid 'Ōhiʻa Death-infected trees from 2018-2019.

This data release includes data and metadata containing (1) study site locations and elevation where bolts (tree stem sections) infected with Ceratocystis lukuohia and/or C. huliohia were collected on Hawai'i Island, (2) the ambrosia beetle (Coleoptera: Scolytinae) species that were reared from bolts, (3) Ceratocystis spp. viability results from culturing tests of the beetles' frass, (4) frass production estimates from three ambrosia beetle species, and (5) quantitative Polymerase Chain Reaction (qPCR) DNA results of viability subcultures. Data were used in the analysis for the manuscript "Ambrosia beetle communities and frass production in ʻōhiʻa (Metrosideros polymorpha) infected with Ceratocystis fungi responsible for Rapid ʻŌhiʻa Death." Beetles and frass were collected in the lab from Rapid 'Ōhiʻa Death-infected trees from 2018-2019.

These data include the ambrosia beetle (Coleoptera: Scolytinae) species that were reared from bolts (tree stem sections) infected with Rapid ʻŌhiʻa Death on Hawaiʻi Island from 2018-2019. These data were used to determine species, number of individuals reared, height, and elevation in which these beetles live.

These data include bark and ambrosia beetle (Coleoptera: Curculionidae) species directly reared from ʻōhiʻa lehua (Metrosideros polymorpha) wood infected with either Ceratocystis lukuohia or Ceratocystis huliohia, the pathogens responsible for Rapid ʻŌhiʻa Death (ROD). Rearing took place on Kauaʻi between January 2020 and November 2021.

These data include bark and ambrosia beetle (Coleoptera: Curculionidae) species directly reared from ʻōhiʻa lehua (Metrosideros polymorpha) wood infected with either Ceratocystis lukuohia or Ceratocystis huliohia, the pathogens responsible for Rapid ʻŌhiʻa Death (ROD). Rearing took place on Kauaʻi between January 2020 and November 2021.

These data include the ambrosia beetle (Coleoptera: Scolytinae) species that were reared from bolts (tree stem sections) infected with Rapid ʻŌhiʻa Death on Hawaiʻi Island from 2018-2019. These data were used to determine species, number of individuals reared, height, and elevation in which these beetles live.

Ambrosia beetles (Coleoptera: Curculionidae) have been implicated in the spread of Ceratocystis fungi that cause rapid ‘ōhiʻa death (ROD), a deadly fungal disease threatening the keystone Hawaiian tree ʻōhiʻa lehua (Metrosideros polymorpha). Research has investigated the role that ambrosia beetles play in the spread of the disease, including studies within Hawaiʻi Volcanoes National Park (HVNP). We collected ambrosia beetles in HVNP and used genetic tools to confirm the morphological taxonomic identification of native and non-native ambrosia beetles. Additionally, we sampled an area in which native ʻōlapa (Cheirodendron tryginum) trees were dying in ʻŌlaʻa Forest to gather taxonomic information on the insect species present. Ambrosia beetles and unknown lepidoptera larva were chiseled from dead and dying trees and we captured ambrosia beetles in panel traps. A subset of individuals (n = 15) were barcoded using Sanger sequencing of the mitochondrial gene cytochrome c oxidase I (COI). Some specimen sequences had matches in the National Center for Biotechnology Information (NCBI) GenBank sequence database when using the query feature, while others did not. For specimens whose sequences did not appear in GenBank queries, we submitted sequence data as a new species entry.

This data release includes data and metadata documenting Ceratocystis viability, moisture content, and beetle activity in felled and tarped ʻōhiʻa lehua (Metrosideros polymorpha) trees previously having been determined to be infected with either Ceratocystis lukuohia or Ceratocystis huliohia, the fungi that cause rapid ʻōhiʻa death (ROD).

This data release includes metadata and tabular data that describes ambrosia beetles that were collected on sticky traps placed on trees inoculated with Ceratocystis lukuohia and on control trees which were not inoculated with the fungus. The dataset includes beetles identified by species and the date on which they were collected. Two sticky traps were placed on each tree.