This data includes the results of an interlab methods comparison to recover and quantify SARS-CoV-2 from wastewater. This dataset is associated with the following publication: Pecson, B., E. Darby, C. Haas, Y. Amha, M. Bartolo, R. Danielson, Y. Dearborn, G. DiGiovanni, C. Ferguson, S. Fevig, E. Gaddis, D. Gray, G. Lukasik, B. Mull, L. Olivas, A. Olivieri, Y. Qu, and S. CoV-2. Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S.. Environmental Science: Water Research & Technology. Royal Society of Chemistry, Cambridge, UK, 7(3): 504-520, (2021).

Data describes percent losses of SARS-CoV-2 from wastewater. Also details recovery of endogenous SARS-CoV-2 from wastewater using two volumetrically different methods. This dataset is associated with the following publication: McMinn, B., A. Korajkic, A. Pemberton, J. Kelleher, W. Ahmed, and E. Villegas. Assessment of two volumetrically different concentration approaches to improve sensitivities for SARS-CoV-2 detection during wastewater monitoring. JOURNAL OF VIROLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 311: 114645, (2023).

This dataset contains the raw droplet counts resulting from of ddPCR or RT-ddPCR of nucleic acid extracts from wastewater. The wastewater was collected from three different sewersheds in Southwest Ohio (Mill Creek WWTP, Taylor Creek WWTP, and a sub-sewershed, Lick Run). ddPCR counts (positive droplets and total droplets) are provided for the following targets: N1 and N2 (SARS-CoV-2 nucleocapsid genes), crAssphage, PMMoV, HF183 (all fecal indicators), and OC43 (an RNA spike-in from a cultured coronavirus). Other metadata (pH, flow, temperature, TSS, CBOD5) are provided where available. This dataset is associated with the following publication: Nagarkar, M., S. Keely, M. Jahne, E. Wheaton, C. Hart, B. Smith, J. Garland, E. Varughese, A. Braam, B. Wiechman, B. Morris, and N. Brinkman. SARS-CoV-2 Monitoring at three sewersheds of different scales and complexity demonstrates distinctive relationships between wastewater measurements and COVID-19 case data. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 816: 151534, (2022).

An interlaboratory assessment of the 8 analytical laboratories participating in the Ohio Wastewater Monitoring Network was conducted. This assessment compared measured results of SARS-CoV-2 gene fragments, fecal indicator organism and matrix spike. The data provided here are the results generated by the EPA/ORD lab participating in the network. This dataset is associated with the following publication: Davis, A., S. Keely, N. Brinkman, Z. Bohrer, Y. Ai, X. Mou, S. Chattopadhyay, O. Hershey, J. Senko, N. Hull , E. Lytmer, A. Quintero, and J. Lee. Evaluation of intra- and inter-lab variability in quantifying SARS-CoV-2 in a state-wide wastewater monitoring network. Environmental Science: Water Research & Technology. Royal Society of Chemistry, Cambridge, UK, 9(4): 1053-1068, (2023).

The City of Bloomington Utilities (CBU) in cooperation with the Indiana Finance Authority is participating in a monitoring program which tests wastewater for coronavirus. From August through December of 2020, wastewater professionals from CBU collected samples three times each week at eight sites across Bloomington, including each of the two wastewater treatment plants. Throughout 2021 samples have been collected from the two wastewater treatment plants. Testing of those samples is conducted by multiple private, independent laboratories in an effort to gauge viral prevalence in the community. More information about this process can be found in the Indiana Wastewater Monitoring Report 2020 at: https://data.bloomington.in.gov/dataset/cd07435a-adae-4ba6-b671-2b711227131d/resource/22b67271-e2d4-4789-af7d-4fc7a586071c/download/indiana-wastewater-monitoring-report-2020_bclear.pdf