Habitat use, diet, prey availability and foraging ecology of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus, Vespertilionidae), was examined in the east Maui region inclusive of the Waihou Mitigation Area, Pu‘u Makua Restoration Area and the wind power facility operated by Auwahi Wind Energy, LLC. The study was conducted to inform the mitigation and management requirements of Auwahi Wind Energy, research funding was provided by Auwahi Wind Energy, LLC (Auwahi Wind) to fulfill requirements for mitigating bat fatalities under its approved incidental take permit. This study included the first genetic analysis of Hawaiian hoary bat diet items, and broadly confirms the major arthropod orders (Coleoptera, Lepidoptera, Diptera, Hemiptera, and Blattodea) found in previous microscopy-based studies of guano samples. Hawaiian hoary bats consumed both native and non-native insect species, and moths were the primary diet component, both in terms of its prevalence among individual bats and proportion of gene sequence counts. A total of 18 Lepidoptera families and 24 genus- or species-level taxa were identified in the analysis.

We examined habitat use and foraging activity of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus), as well as nocturnal aerial insect abundance at Kaloko-Honōkohau National Historical Park located in the coastal region of Kailua-Kona, Hawai‘i Island. The study area covers approximately 486 ha of marine waters and terrestrial coastal plains. Nocturnal aerial insect collection occurred in January and April (2 or 3 nights each) and in July and November (1 or 2 nights each) in 2014. Insects were collected at three stations (KAHO1, KAHO3, KAHO4) between 1 and 10 m above sea level. Nocturnal aerial insects were collected using a 22 W UV light placed above a funnel and bucket trap (Model #2851M, Bioquip Products Inc., Rancho Dominguez, CA) and powered by a 12 V marine deep cycle battery. Insect traps were placed near (~10 m) KAHO1 and KAHO3 (wooded shorelines) and KAHO4 (xeric, vegetated lava field). Timers turned the lights on at approximately one hour before local sunset and off at approximately one hour after local sunrise. Insects were removed from the traps at approximately 00:00 and 06:00, and preserved in EtOH. Insect samples were counted and identified to taxonomic order under a dissecting microscope (Leica MS5, Leica Microsystems Inc., Wetzlar, DE). Samples were dried in a drying oven for approximately 48 hours at 65 C and weighed on an analytical scale.

We examined habitat use and foraging activity of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus), as well as nocturnal aerial insect abundance at Kaloko-Honōkohau National Historical Park located in the coastal region of Kailua-Kona, Hawai‘i Island. The study area covers approximately 486 ha of marine waters and terrestrial coastal plains. Nocturnal aerial insect collection occurred in January and April (2 or 3 nights each) and in July and November (1 or 2 nights each) in 2014. Insects were collected at three stations (KAHO1, KAHO3, KAHO4) between 1 and 10 m above sea level. Nocturnal aerial insects were collected using a 22 W UV light placed above a funnel and bucket trap (Model #2851M, Bioquip Products Inc., Rancho Dominguez, CA) and powered by a 12 V marine deep cycle battery. Insect traps were placed near (~10 m) KAHO1 and KAHO3 (wooded shorelines) and KAHO4 (xeric, vegetated lava field). Timers turned the lights on at approximately one hour before local sunset and off at approximately one hour after local sunrise. Insects were removed from the traps at approximately 00:00 and 06:00, and preserved in ethyl alcohol (EtOH). Insect samples were counted and identified to taxonomic order under a dissecting microscope (Leica MS5, Leica Microsystems Inc., Wetzlar, DE). Samples were dried in a drying oven for approximately 48 hours at 65 C and weighed on an analytical scale.

Habitat use, diet, prey availability and foraging ecology of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus, Vespertilionidae) were examined in the east Maui region inclusive of the Waihou Mitigation Area, Pu‘u Makua Restoration Area and the wind power facility operated by Auwahi Wind Energy, LLC. The study was conducted to inform the mitigation and management requirements of Auwahi Wind Energy, and fulfill requirements for mitigating bat fatalities under its approved incidental take permit. Acoustic monitoring over the three-year period demonstrated that bats are present and actively feed year-round at the Waihou Mitigation Area. This study included the first genetic analysis of Hawaiian hoary bat diet items, and broadly confirms the major arthropod orders (Coleoptera, Lepidoptera, Diptera, Hemiptera, and Blattodea) found in previous microscopy-based studies of guano samples.

Habitat use, diet, prey availability and foraging ecology of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus, Vespertilionidae) were examined in the east Maui region inclusive of the Waihou Mitigation Area, Pu‘u Makua Restoration Area and the wind power facility operated by Auwahi Wind Energy, LLC. The study was conducted to inform the mitigation and management requirements of Auwahi Wind Energy, and fulfill requirements for mitigating bat fatalities under its approved incidental take permit. Acoustic monitoring over the three-year period demonstrated that bats are present and actively feed year-round at the Waihou Mitigation Area. This study included the first genetic analysis of Hawaiian hoary bat diet items, and broadly confirms the major arthropod orders (Coleoptera, Lepidoptera, Diptera, Hemiptera, and Blattodea) found in previous microscopy-based studies of guano samples.

Habitat use, diet, prey availability and foraging ecology of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus, Vespertilionidae), was examined in the east Maui region inclusive of the Waihou Mitigation Area, Pu‘u Makua Restoration Area and the wind power facility operated by Auwahi Wind Energy, LLC. Research funding was provided to conduct the bat ecology study by Auwahi Wind Energy, LLC, in order to fulfill requirements for mitigating bat fatalities under its approved incidental take permit. This study included the first genetic analysis of Hawaiian hoary bat guano items, and broadly confirms the major arthropod orders (Coleoptera, Lepidoptera, Diptera, Hemiptera, and Blattodea) found in previous microscopy-based studies of guano samples. This dataset describes the representative DNA sequences of operational taxonomic units (OTU) of prey items found in bat guano, derived with two different primers (Zeale, Epp).

Habitat use, diet, prey availability and foraging ecology of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus, Vespertilionidae), was examined in the east Maui region inclusive of the Waihou Mitigation Area, Pu‘u Makua Restoration Area and the wind power facility operated by Auwahi Wind Energy, LLC. Research funding to study bat ecology was provided by Auwahi Wind Energy, LLC in order to fulfill requirements for mitigating bat fatalities under it's approved incidental take permit. This study included the first genetic analysis of Hawaiian hoary bat diet items, and broadly confirms the major arthropod orders (Coleoptera, Lepidoptera, Diptera, Hemiptera, and Blattodea) found in previous microscopy-based studies of guano samples. This dataset describes the representative DNA sequences of operational taxonomic units (OTU) of prey items found in bat guano, derived with the Epp primer and it's count sequence reads in each bat sample.

Habitat use, diet, prey availability and foraging ecology of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus, Vespertilionidae), was examined in the east Maui region inclusive of the Waihou Mitigation Area, Pu‘u Makua Restoration Area and the wind power facility operated by Auwahi Wind Energy, LLC .Research for the bat ecology study was provided by Auwahi Wind Energy, LLC, in order to fulfill requirements for mitigating bat fatalities under its approved incidental take permit. Acoustic monitoring over the three-year period demonstrated that bats are present and actively feed year-round at the Waihou Mitigation Area. This study included the first genetic analysis of Hawaiian hoary bat diet items, and broadly confirms the major arthropod orders (Coleoptera, Lepidoptera, Diptera, Hemiptera, and Blattodea) found in previous microscopy-based studies of guano samples.

Habitat use, diet, prey availability and foraging ecology of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus, Vespertilionidae), was examined in the east Maui region inclusive of the Waihou Mitigation Area, Pu‘u Makua Restoration Area and the wind power facility operated by Auwahi Wind Energy, LLC .Research for the bat ecology study was provided by Auwahi Wind Energy, LLC, in order to fulfill requirements for mitigating bat fatalities under its approved incidental take permit. Acoustic monitoring over the three-year period demonstrated that bats are present and actively feed year-round at the Waihou Mitigation Area. This study included the first genetic analysis of Hawaiian hoary bat diet items, and broadly confirms the major arthropod orders (Coleoptera, Lepidoptera, Diptera, Hemiptera, and Blattodea) found in previous microscopy-based studies of guano samples.

Habitat use, diet, prey availability and foraging ecology of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus, Vespertilionidae), was examined in the east Maui region inclusive of the Waihou Mitigation Area, Pu‘u Makua Restoration Area and the wind power facility operated by Auwahi Wind Energy, LLC. Research for the bat ecology study was provided by Auwahi Wind Energy, LLC, in order to fulfill requirements for mitigating bat fatalities under its approved incidental take permit. This study included the first genetic analysis of Hawaiian hoary bat guano items, and broadly confirms the major arthropod orders (Coleoptera, Lepidoptera, Diptera, Hemiptera, and Blattodea) found in previous microscopy-based studies of guano samples. This dataset describes the representative DNA sequences of operational taxonomic units (OTU) of prey items found in bat guano, derived with two different primers (Zeale, Epp).