Genome assemblies and sequencing data for two Rhizoctonia isolates, AG8-1 and AG8-3.

Compute cDNA-to-Genomic sequence alignments.

,Within this dataset:,Linum lewisii, a perennial blue flax native to North America, holds potential as a sustainable perennial crop for oilseed production due to its ecological adaptability, upright harvestable structure, nutritious seeds, and low insect and disease issues. Its native distribution spans a large geographic range, from the Pacific Coast to the Mississippi River, and from Alaska to Baja California. Tolerant to cold and drought conditions, this species is also important for native ecosystem rehabilitation. Its enhancement of soil health, support for pollinators, and carbon sequestration underscore its agricultural relevance.,This study presents a high-quality, chromosome-scale assembly of the L. lewisii (2n = 2x = 18) genome, derived from PacBio HiFi and Dovetail Omni-C sequencing of the “Maple Grove” variety. The initial assembly contained 642,903,787 base pairs across 2,924 scaffolds. Following HiRise scaffolding, the final assembly contained 643,041,835 base pairs, across 1,713 scaffolds, yielding an N50 contig length of 66,209,717 base pairs. Annotation of the assembly revealed 38,808 genes, including 37,599 protein-coding genes and 7,108 putative transposable elements. Analysis of synteny with other flax species revealed a striking number of chromosomal rearrangements. We also found an intriguing absence of the single-copy TSS1 gene in the L. lewisii genome, potentially linked to its transition from heterostyly to homostyly.,Taken together, these findings represent a significant advancement in our understanding of the Linum genus and provide a resource for future domestication efforts and basic research on Lewis flax.,

,The data in this repository is to provide the scripts used in the assembly of the Anastrepha ludens genome. It can be used to replicate the steps necessary to assemble the genome described in National Center for Biotechnology Information (NCBI) BioProject: PRJNA803324. This genome assembly workflow was performed on the United States Department of Agriculture (USDA) - SCINet/Mississippi State University High Performance Computing Cluster (HPC) Atlas and installed via Conda.,,

This genetic variation services interconvert and transform short genetic variants between HGVS expressions, VCF format, and the new SPDI (Sequence Position Deletion Insertion) format, based on alignment datasets used by ClinVar and dbSNP. NOTE: This service is still in beta testing mode

,The genosim package simulates genotypes, breeding values, and phenotypes; simulates DNA sequence read depth (numbers of A and B alleles); and resolves SNP conflicts between parent and offspring genotypes.,,

Metagenome Assembled Genome (MAG) of Sphingomonas sanguinis from the International Space Station metagenome

,The Baylor College of Medicine recently sequenced and annotated the Leptinotarsa decemlineata genome as part of the i5k pilot project. This dataset presents the Leptinotarsa decemlineata genome v1.0. This assembly version is the pre-release version, prior to filtering and quality control by the National Center for Biotechnology Information's GenBank resource. Assembly method details will be available in a forthcoming publication.,The Colorado potato beetle is considered the economically most significant defoliator of potato in northern latitudes worldwide. The range of this insect is continuing to expand, and it is likely to eventually colonize all potato-producing areas with temperate climate. Within it's native habitat, the beetle feeds on native solanaceous plants, S. angustifolium, S. elaeagnifolium, and buffalo bur, S. rostratum. However, it has adapted to potatoes and other solanaceous crops after its range expansion.,Due to the lack of any natural enemies that have been able to evolve seasonal adaptations, the cornerstone of Colorado potato beetle management has been the use of insecticides. However, the beetle has shown a remarkable ability to develop resistance to most insecticides used for its control. The mechanism(s) of insecticide resistance is yet unknown and genomic sequencing will lead to major advances in managing this pest in commercial plant production.,If you wish to use this dataset, please follow the Baylor College of Medicine's conditions for data use: https://www.hgsc.bcm.edu/bcm-hgsc-conditions-use,

,The Leptinotarsa decemlineata genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. This dataset presents the Leptinotarsa decemlineata gene set BCM_v_0.5.3, which was generated computationally. RNA-Seq data was used with additional protein homology data for a MAKER automated annotation of the Leptinotarsa decemlineata genome assembly 1.0. Further annotation method details will be available in a forthcoming publication.,NOTE: This gene set is an unstable pre-release (v0.5.3), and was provided to facilitate manual curation and analyses before the official gene set is released. Gene identifiers from this gene set will likely not be maintained.,If you wish to use this dataset, please follow the Baylor College of Medicine's conditions for data use: https://www.hgsc.bcm.edu/bcm-hgsc-conditions-use,