,This dataset contains manual annotations from Rhyzopertha dominica community curators, based on genome assembly RdoDt3_Drdd8_decomES.fasta.gz. These annotations are direct exports from Apollo 2.6 (https://doi.org/10.5281/zenodo.5015109), hosted by the i5k Workspace@NAL (https://i5k.nal.usda.gov/). Manual annotations are temporary and will be reviewed by the i5k Workspace@NAL and submitted to NCBI's GenBank database after review.,

The Melampsora lini genome was sequenced to improve its genome assembly. PacBio HiFi and Hi-C data was generated as well as RNA-seq data.

Background: In nitrate-poor soils, many leguminous plants form nitrogen-fixing symbioses with members of the bacterial family Rhizobiaceae. We selected Rhizobium sp. NGR234 for its exceptionally broad host range, which includes more than I 12 genera of legumes. Unlike the genome of Bradyrhizobium japonicum, which is composed of a single 8.7 Mb chromosome, that of NGR234 is partitioned into three replicons: a chromosome of about 3.5 Mb, a megaplasmid of more than 2 Mb (pNGR234b) and pNGR234a, a 536,165 bp plasmid that carries most of the genes required for symbioses with legumes. Symbiotic loci represent only a small portion of all the genes coded by rhizobial genomes, however. To rapidly characterize the two largest replicons of NGR234, the genome of strain ANU265 (a derivative strain cured of pNGR234a) was analyzed by shotgun sequencing. Results: Homology searches of public databases with 2,275 random sequences of strain ANU265 resulted in the identification of 1,130 putative protein-coding sequences, of which 922 (41%) could be classified into functional groups. In contrast to the 18% of insertion-like sequences (ISs) found on the symbiotic plasmid pNGR234a, only 2.2% of the shotgun sequences represent known ISs, suggesting that pNGR234a is enriched in such elements. Hybridization data also indicate that the density of known transposable elements is higher in pNGR234b (the megaplasmid) than on the chromosome. Rhizobium-specific intergenic mosaic elements (RIMEs) were found in 35 shotgun sequences, 6 of which carry RIME2 repeats previously thought to be present only in Rhizobium meliloti. As non-overlapping shotgun sequences together represent approximately 10% of ANU265 genome, the chromosome and megaplasmid may carry a total of over 200 RIMEs. Conclusions: 'Skimming' the genome of Rhizobium sp. NGR234 sheds new light on the fine structure and evolution of its replicons, as well as on the integration of symbiotic functions in the genome of a soil bacterium. Although most putative coding sequences could be distributed into functional classes similar to those in Bacillus subtilis, functions related to transposable elements were more abundant in NGR234. In contrast to ISs that accumulated in pNGR234a and pNGR234b, the hundreds of RIME elements seem mostly attributes of the chromosome.

Briefly, sample collection will entail collecting soil samples at 5 sites within 1 suggested area, with each site being more than a quarter mile apart. Sample sites would be between 150 feet from roads, and 75 feet from trails. At each site, 3 subsamples of topsoil (0-8 inches) using scoop will be collected, yielding 1 pooled sample (about 1.2 lbs).

,Our mission is to support application of low-input sustainable agriculture by: Providing, to the best of our ability, technical information about rhizobia, their preservation, and cultural and symbiotic characteristics; Acquiring and preserving the nitrogen-fixing bacterial symbionts of leguminous plants with the goal of maintaining widest possible genetic diversity; Maintaining quality control of new and existing germplasm by evaluation of microbiological purity and by examination of nodulation of the original trap host plant; Distributing cultures to the public and private sectors without charge for these services; Developing or adapting techniques in molecular biology for the determination of genetic diversity of rhizobia, to investigate interactions with their host plants and to identify novel characteristics; Acquiring, maintaining, evaluating quality, and distributing type strains for all the different taxa of nitrogen-fixing legume symbionts; Participating in the UNESCO program.,

Draft Genome sequences of Rhodotorula mucilaginosa isolated from the International space station.

These data show multilocus genotypes for Chylismia brevipes sampled from natural populations throughout the Mojave Desert of California, Nevada, and Arizona from 2016 to 2019. The data release contains two vcf files with SNPs from single-end Restriction-site associated DNA sequencing (RADSeq), which differ in the restriction enzyme used during sequencing. One file contains PstI genotypes, and the other SbfI genotypes.