Briefly, sample collection will entail collecting soil samples at 5 sites within 1 suggested area, with each site being more than a quarter mile apart. Sample sites would be between 150 feet from roads, and 75 feet from trails. At each site, 3 subsamples of topsoil (0-8 inches) using scoop will be collected, yielding 1 pooled sample (about 1.2 lbs).

These data represent the first reference genome for the invasive Phragmites australis ssp. australis (1.14 giga base pairs (Gbp)), as well as output from comparative genomic and transcriptomic analyses for invasive and native genotypes coexisting in the Great Lakes region of North America. Genome sequencing data used tillers and associated rhizome tissues collected from a single P. australis patch at the Ottawa National Wildlife Refuge near Toledo, Ohio, USA. Transcriptome analyses were produced from samples collected from three invasive and three native genotype P. australis patches from four sites around the Great Lakes in Michigan and Ohio, USA.

These data represent the first reference genome for the invasive Phragmites australis ssp. australis (1.14 giga base pairs (Gbp)), as well as output from comparative genomic and transcriptomic analyses for invasive and native genotypes coexisting in the Great Lakes region of North America. Genome sequencing data used tillers and associated rhizome tissues collected from a single P. australis patch at the Ottawa National Wildlife Refuge near Toledo, Ohio, USA. Transcriptome analyses were produced from samples collected from three invasive and three native genotype P. australis patches from four sites around the Great Lakes in Michigan and Ohio, USA.

The GRSM exotic plants database is a multi-faceted, SQL-based database containing location and treatment information for the exotic invasive plant control program. The exotic plant site location information includes plant species treated, geospatial information (point and polygon), site directions, and site notes. The exotic plant treatment information includes treatment method, herbicide and adjuvant brands and amounts used, area treated, field notes, and employees present. The purpose of this database is to allow easy site re-location for multi-year treatments, easy treatment data sums and sorting for end of season and project-based reporting, tracking of long-term trends in exotic invasive species management, and to make exotic plant work scheduling more efficient. GRSM manages over 50 invasive exotic plant species in over 900 locations parkwide with treatments records in this database starting in 1994. The National Park Service has published these data as a stand-alone export from an enterprise database. While the nature of table relationships and primary/foreign keys is detailed in this document, the National Park Service cannot provide assitance or instruction in the use of propietary software in analyzing and manipulating these data. Users should consult their software documention for instructions on manipulating these data. These GIS data are companion to a NRDT database, and users should consult NRDT documentation for instructions on viewing these data (Dietrich CH. 2005. Natural Resource Database Template Core Tables Standard. National Park Service (NPS). Geospatial Dataset-1035640.) (https://irma.nps.gov/App/Reference/Profile/1035640)(http://science.nature.nps.gov/im/datamgmt/applications/template/index.cfm)

The GRSM exotic plants database is a multi-faceted, SQL-based database containing location and treatment information for the exotic invasive plant control program. The exotic plant site location information includes plant species treated, geospatial information (point and polygon), site directions, and site notes. The exotic plant treatment information includes treatment method, herbicide and adjuvant brands and amounts used, area treated, field notes, and employees present. The purpose of this database is to allow easy site re-location for multi-year treatments, easy treatment data sums and sorting for end of season and project-based reporting, tracking of long-term trends in exotic invasive species management, and to make exotic plant work scheduling more efficient. GRSM manages over 50 invasive exotic plant species in over 900 locations parkwide with treatments records in this database starting in 1994. The National Park Service has published these data as a stand-alone export from an enterprise database. While the nature of table relationships and primary/foreign keys is detailed in this document, the National Park Service cannot provide assitance or instruction in the use of propietary software in analyzing and manipulating these data. Users should consult their software documention for instructions on manipulating these data. These GIS data are companion to a NRDT database, and users should consult NRDT documentation for instructions on viewing these data (Dietrich CH. 2005. Natural Resource Database Template Core Tables Standard. National Park Service (NPS). Geospatial Dataset-1035640.) (https://irma.nps.gov/App/Reference/Profile/1035640)(http://science.nature.nps.gov/im/datamgmt/applications/template/index.cfm)

Field Methods: I will collect individual sample branches (one for each population to make specimens) and few leaves for the other species without hurting the plants. I would only need population samples for Streptopus lanceolatus, Goodyera pubescens and Gaultheria procumbens. The leaves will be dried in silica gel and for DNA extraction. The species include: Prosartes languinosa, Prosartes matculata, Goodyera pubescens, Gaultheria procumbens, Streptopus amplexifolius, Streptopus lanceolatus.

Genome assemblies and sequencing data for two Rhizoctonia isolates, AG8-1 and AG8-3.

The fasta files (Genome_Set01.zip) contain the reference-assisted de novo assemblies (as contigs) of four Campylobacter spp. isolates. The table contains rows as isolates (yellow) and columns as attributes (green) for each individual genome. This dataset is associated with the following publication: Gomez-Alvarez, V., N. Ashbolt, J. Griffith, J. Santo Domingo, and J. Lu. Whole-Genome Sequencing of Four Campylobacter strains Isolated from Gull Excreta collected from Hobie Beach (Oxnard, CA, USA). Microbiology Resource Announcements. American Society for Microbiology, Washington, DC, USA, 8(32): e00560-19, (2019).

This study generated genetic 'metabarcode' data using high-throughput sequencing to characterize pollen foraging behavior of the endangered rusty-patched bumblebee, Bombus affinis. Pollen samples were collected primarily from forest meadow habitats in the Appalachian piedmont of the eastern United States, specifically within the states of Virginia and West Virginia. Three additional samples from the upper Midwest were also included for comparison. This data release consists of three tab-delimited files and a file of DNA sequences: 1) sample.metadata.txt includes sample identifiers and the accessions they have been assigned by the National Center for Biotechnology Information (NCBI), the authoritative repository for publicly funded genetic data in the United States. These accessions can be used individually to obtain raw sequencing data or sample information at www.ncbi.nlm.nih.gov. Alternatively, the BioProject accession PRJNA1235776 can be searched to retrieve the full set of data and sample accessions listed in the file. Entity and attribute metadata are provided for this file herein. 2) ITS1.raw.pollen.counts.txt includes the inferred taxon counts at the internal transcribed spacer 1 (ITS1) genetic locus, i.e. number of ITS1 sequences in a sample attributable to each identified taxon in each sample. Taxa are in rows and sequencing libraries are in columns. Taxa are listed by scientific name and the taxonomic rank of that scientific name. A numeric taxonomic identifier used by NCBI for each taxon is also provided, as the taxonomic identifier is unique in the NCBI databases whereas scientific names may not be. Entity and attribute data are not provided for this file due to its size and repetitive content. 3) ITS2.raw.pollen.counts.txt includes the inferred taxon counts at the internal transcribed spacer 2 (ITS2) genetic locus, i.e. number of ITS2 sequences in a sample attributable to each identified taxon in each sample. The file is identical in structure to the ITS1 file. 4) reference.db.fas contains the plant reference DNA sequences used for taxonomic assignment of the pollen sample sequences.

Field Methods: I will sample salamanders at five locations within Great Smoky Mountains National Park. Collections will take place during June 2015. D. quadramaculatus and D. marmoratus will be located by visually searching streams, stream edges, and beneath rocks within streams. All efforts will be made to minimize impact on the habitat. Each salamander found will be hand-captured and placed in a new, clean plastic bag with a small amount of water to keep their skin moist. Salamanders will then be measured with calipers for snout-vent length, tail length, and cranial length and width. An approximately 5 mm piece of tissue will be removed from the tail tip for use in genetic analyses. To minimize handling stress, salamander measurements and tissue collection will take place in the field, adjacent to capture sites, and salamanders will be released at their point of capture immediately afterwards.