This dataset estimates human mobility through origin destination (OD) movement flow among the Statistical Area 2 (SA2) regions in Queensland (QLD), connected by public transport (PT) networks. The SA2 regions of Queensland connected by buses, trains, trams and ferries have been used to evaluate OD movement flows. The passenger OD movement data among different stations (or the station-based OD flow) are first estimated using a statistical estimation methodology. The stations-based OD flow data are then translated into region-based OD matrices using the state-of-art method. For more information please see the original metadata file here. Human mobility data is a key ingredient in various areas and domains of research including epidemiology, policy and administration, criminology, transportation, logistics and supply chains, environmental management and, pollution and contamination. High quality human mobility data provided by telecommunication companies collected from call data records (CDRs) is available at prohibitive cost with restrictive licensing, keeping it out of reach for the majority of research community. On the other hand, there is an abundance of high-quality public data, reporting different aspects of mobility. Examples are the public transport patronage and information about the usage of the Australian road network. These datasets are collected by different organisations and government departments and are presented in various formats. For instance, data may be collected at different spatial (e.g. at state or postcode levels) and temporal scales and be presented in the form of passenger counts or aggregated movement flows. This dataset addresses the general lack of national scale comprehensive human mobility dataset in Australia by transforming available mobility data into a consistent format that is suitable for analysis in a broad range of research areas. Merging the various individual datasets into Australia's first comprehensive, national-scale human mobility data asset drastically improves the quality and coverage of existing datasets. The Mobility Australia project received investment (https://doi.org/10.47486/DP702) from the Australian Research Data Commons (ARDC). The ARDC is funded by the National Collaborative Research Infrastructure Strategy (NCRIS). The original data tables were structured in a matrix-like format. AURIN employed a methodology to merge diverse datasets into a comprehensive one, categorising based on transportation types (e.g., trains, buses, rails, ferries), years (e.g., 2019, 2020, 2021, etc.), and temporal scales (e.g., weekly, monthly, yearly). Subsequently, AURIN spatially enabled the original data by employing the 2021 edition of the Australian Statistical Geography Standard (ASGS). The flow between origin and destination pairs is visually represented using line geometry.