This dataset includes laboratory data collected to determine the effects of hydrogen peroxide on nitrification processes in biofilters of recirculating aquaculture systems (RAS). Data from the laboratory studies were used to inform methods used to conduct field trials in pilot- and commercial-scale RAS. Biofilter nitrification was monitored by measuring the levels of total ammonia-nitrogen, nitrite-nitrogen, and nitrate-nitrogen in experimental aquaria containing a small biofilter before and after hydrogen peroxide was applied to the test system. There are 4 excel workbooks associated with this data set: RASHP Range finding Trial.csv, RASHP Definitive Trial.csv, Hydrogen Peroxide Lab Studies.csv, and DO after HP addition.csv

This dataset includes laboratory data collected to determine the effects of hydrogen peroxide on nitrification processes in biofilters of recirculating aquaculture systems (RAS). Data from the laboratory studies were used to inform methods used to conduct field trials in pilot- and commercial-scale RAS. Biofilter nitrification was monitored by measuring the levels of total ammonia-nitrogen, nitrite-nitrogen, and nitrate-nitrogen in experimental aquaria containing a small biofilter before and after hydrogen peroxide was applied to the test system. There are 4 excel workbooks associated with this data set: RASHP Range finding Trial.csv, RASHP Definitive Trial.csv, Hydrogen Peroxide Lab Studies.csv, and DO after HP addition.csv

The ability of formalin, PEROX-AID® (hydrogen peroxide), and seawater to kill waterborne Nanophyetus salmincola cercariae was evaluated in vitro. Newly emerged waterborne cercariae survived for extended periods in freshwater, with 50-74% survival occurring in negative control groups after 24 hr. Exposure to dilutions of formalin reduced this survival time, with 0% of cercariae surviving after 30 min. in 167 ppm, 40 min. in 83 ppm, and 180 min. in 42ppm. Exposure to PEROX-AID® (hydrogen peroxide) for one hour resulted in reduced cercariae survival (16.4%) only at the highest concentration (100 ppm), compared to 100% survival in the untreated controls and all lesser concentrations. Dilutions of seawater resulted in reduced cercariae survival only at high salinities (15.2 – 30.3 ‰), where 10 min. exposures resulted in 0 – 16% survival. These results provide insights into prophylactic water treatment options for salmonid enhancement facilities that experience high mortalities due to infections with Nanophyetus salmincola. Further, the intolerance of cercariae to high salinities indicates that exposures of fish to infectious waterborne cercariae occur primarily in the freshwater portions of watersheds.

The ability of formalin, PEROX-AID® (hydrogen peroxide), and seawater to kill waterborne Nanophyetus salmincola cercariae was evaluated in vitro. Newly emerged waterborne cercariae survived for extended periods in freshwater, with 50-74% survival occurring in negative control groups after 24 hr. Exposure to dilutions of formalin reduced this survival time, with 0% of cercariae surviving after 30 min. in 167 ppm, 40 min. in 83 ppm, and 180 min. in 42ppm. Exposure to PEROX-AID® (hydrogen peroxide) for one hour resulted in reduced cercariae survival (16.4%) only at the highest concentration (100 ppm), compared to 100% survival in the untreated controls and all lesser concentrations. Dilutions of seawater resulted in reduced cercariae survival only at high salinities (15.2 – 30.3 ‰), where 10 min. exposures resulted in 0 – 16% survival. These results provide insights into prophylactic water treatment options for salmonid enhancement facilities that experience high mortalities due to infections with Nanophyetus salmincola. Further, the intolerance of cercariae to high salinities indicates that exposures of fish to infectious waterborne cercariae occur primarily in the freshwater portions of watersheds.

This dataset includes laboratory data collected to determine the effects of formaldehyde on nitrification processes in biofilters of recirculating aquaculture systems (RAS). Data from the laboratory studies were used to inform methods used to conduct field trials in a pilot and commercial-scale RAS. Biofilter nitrification was monitored by measuring the levels of total ammonia nitrogen, nitrite, and nitrate in experimental aquaria containing a small biofilter before and after formaldehyde was applied to the test system.

This dataset includes data collected to determine the effects of formaldehyde on nitrification processes in biofilters of recirculating aquaculture systems (RAS) from two field trials. Biofilter nitrification was monitored by measuring the levels of total ammonia nitrogen, nitrite, and nitrate in experimental aquaria containing a small biofilter before and after formaldehyde was applied to the test system.

This dataset includes data collected to determine the effects of formaldehyde on nitrification processes in biofilters of recirculating aquaculture systems (RAS) from two field trials. Biofilter nitrification was monitored by measuring the levels of total ammonia nitrogen, nitrite, and nitrate in experimental aquaria containing a small biofilter before and after formaldehyde was applied to the test system.

This dataset includes data collected to determine the effects of formaldehyde on nitrification processes in biofilters of recirculating aquaculture systems (RAS) from two field trials. Biofilter nitrification was monitored by measuring the levels of total ammonia nitrogen, nitrite, and nitrate in experimental aquaria containing a small biofilter before and after formaldehyde was applied to the test system.