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Molecular Detection of Legionella spp. and their associations with Mycobacterium spp., Pseudomonas aeruginosa and amoeba hosts in a drinking water distribution system
Quantity of Legionella spp., Mycobacterium spp., Acanthamoeba,Vermamoeba vermiformis and Pseudomonas aeruginosa were estimated using qPCR methods. This dataset is associated with the following publication: Lu , J., I. Struewing, E. Vereen, A.E. Kirby, K. Levy, C. Moe, and N. Ashbolt. Molecular detection of Legionella spp. and their associations with Mycobacterium spp., Pseudomonas aeruginosa and amoeba hosts in a drinking water distribution system (Journal Article). JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 120(2): 509-521, (2016).
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Molecular Detection of Legionella spp. and their associations with Mycobacterium spp., Pseudomonas aeruginosa and amoeba hosts in a drinking water distribution system
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Quantity of Legionella spp., Mycobacterium spp., Acanthamoeba,Vermamoeba vermiformis and Pseudomonas aeruginosa were estimated using qPCR methods. This dataset is associated with the following publication: Lu , J., I. Struewing, E. Vereen, A.E. Kirby, K. Levy, C. Moe, and N. Ashbolt. Molecular detection of Legionella spp. and their associations with Mycobacterium spp., Pseudomonas aeruginosa and amoeba hosts in a drinking water distribution system (Journal Article). JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 120(2): 509-521, (2016).
raw data collected from Malvern Instrument
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These are raw data/image files from the Malvern Zetasizer Instrument. This dataset is associated with the following publication: Buse, H., J. Hoelle, C. Muhlen, and D. Lytle. Electrophoretic mobility of Legionella pneumophila serogroups 1 to 14. FEMS MICROBIOLOGY LETTERS. Elsevier Science Ltd, New York, NY, USA, 1, (2018).
Avium Legionella and Structures
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Concentrations of Legionella pneumphila and Mycobacterium avium as measured by quantitative Polmerase chaine reaction. This dataset is associated with the following publication: Donohue, M., D. King, S. Pfaller, and J. Mistry. The sporadic nature of Legionella pneumophila, Legionella pneumophila Sg1 and Mycobacterium avium occurrence within residences and office buildings across 36 states in the United States. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 126(5): 1568-1579, (2019).
Legionella-biofilm dataset
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Water quality measurements, disinfectant decay during experiments, raw data, qPCR data. This dataset is associated with the following publication: Buse, H., J. Szabo, B. Morris, and I. Struewing. Chlorine and monochloramine disinfection of Legionella pneumophila colonizing copper and PVC drinking water biofilms. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, USA, 1-33, (2019).
Legionella propagation and staining
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Pictures of live/dead Legionella pneumophila under confocal microscope before and after treatment. This dataset is associated with the following publication: Oh, Y., R. Noga, V. Shanov, H. Ryu, H. Chandra, B. Yadav, J. Yadav, and S. Chae. Simultaneous Separation and Inactivation of Legionella pneumophila using Electrically Heatable Carbon Nanotube Sheet Composite Membranes. Chemical Engineering Journal. Elsevier BV, AMSTERDAM, NETHERLANDS, 366: 21-26, (2019).
LPMA Hot Water
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This dataset contains the molecular occurrence and concentration of Legionella pneumophila, M. avium, M. intracellulare and M. abscessus by structure type. Portions of this dataset are inaccessible because: This data set will have a second paper that is not written yet. Please hold off publishing the data set until the second paper is published. They can be accessed through the following means: This data will be accessible via Science Hub at a later date. Format: Molecular Data. This dataset is associated with the following publication: Donohue, M., J. Mistry, N. Tucker, and S. Vesper. Hot Water in Residences and Office Buildings Have Distinctive Legionella pneumophila Contamination Risk. . (ed.), INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH. Elsevier B.V., Amsterdam, NETHERLANDS, 245: 114023, (2022).
Annual variations and effects of temperature on Legionella spp. and other potential opportunistic pathogens in tap and shower water
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The data contained in this worksheet provides the quantitative detection of potential pathogens for the bathroom water samples used in this study. This dataset is associated with the following publication: Lu, J., H. Buse, I. Struewing, A. Zhao, D. Lytle, and N. Ashbolt. Annual variations and effects of temperature on Legionella spp. and other potential opportunistic pathogens in tap and shower water. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 24(0): 2326-2336, (2016).
Raw data of Legionella pneumophila determined by culture and Legiolert methods
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This Excel file contains all the raw data of Legionella pneumophila determined by both culture and Legiolert methods. Datasheets were prepared separately for each figure and table. This dataset is associated with the following publication: Boczek, L., M. Tang, C. Formal, D. Lytle, and H. Ryu. Comparison of two culture methods for the enumeration of Legionella pneumophila from potable water samples. JOURNAL OF WATER AND HEALTH. IWA Publishing, London, UK, 19(3): 468-477, (2021).
Data from: Detection of viral, bacterial, and protozoan pathogens and microbial source tracking markers in paired large- and small-volume water samples
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,This repository contains data supporting the publication, "Detection of viral, bacterial, and protozoan pathogens and microbial source tracking markers in paired large- and small-volume water samples." The dataset comprises qPCR concentrations of microbial targets in paired large volume and small volume samples from field studies and laboratory recovery experiments, as described in the main publication.,"Field studies.csv" contains sample data and target concentrations for three field studies, two in groundwater and one in surface water. In the "Groundwater - Private wells (n = 138)" study, large volume samples taken by dead-end ultrafiltration were compared to small volume grab samples. In the remaining two studies, large volume dead-end ultrafiltration and small volume samples were collected synchronously to remove the effect of spatial/temporal heterogeneity during collection. Only the 15 qPCR assays with detections and common to all studies are included. Geographic information is not included to protect the privacy of study participants.,"Recovery data.csv" contains laboratory data for large volume and small volume method recovery of three microbial targets (a bacterium, a virus, and a protozoan) at varying initial concentrations.,"Storage data.csv" contains laboratory data for microbial target decay during storage prior to processing. Liquid small volume samples and large volume ultrafilters were stored at 4 C to simulate normal sample storage and transport conditions. Concentrations of three microbial targets (a bacterium, a virus, and a protozoan) were determined for storage times from 0 to 96 hours.,Descriptive data for all microbial targets is provided in "Target data.csv". An explanation of variables (with units) for all data files can found in "Key to variables.csv".,