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Genetic fingerprinting of 184 Aspergillus from Ethiopia isolated in 2015 from peanut seeds, raw data
,Genetic fingerprinting of 184 Aspergillus section Flavi isolates from Ethiopia screened with 24 Insertion/Deletion markers located within the aflatoxin-biosynthesis gene cluster. Each file name contains in this order: isolate number, marker number, range of base pairs on the aflatoxin-biosynthesis cluster where the marker is located, and well position within the 384 microplate used for capillary electrophoresis.,,
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Raw Sequences of 17 Aspergillus Genomes from Ethiopia
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,Raw genome sequencing data of 17 isolates of Aspergillus flavus and Aspergillus parasiticus collected from peanuts from Ethiopia. These isolates were selected as representative of the genetic diversity of Aspergillus section Flavi found colonizing peanut seeds in four peanut-farming districts of Ethiopia, these are Darolabu, Gursum, Fedis, and Babile.,,
Genomic sequences of Aspergillus flavus accessions in Georgia USA
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The data was produced as part of the Feed the Future Innovation Lab for Collaborative Research on Peanut Productivity and Aflatoxin Control (the Peanut & Mycotoxin Innovation Lab, PMIL) subaward to the USDA Agricultural Research Service's National Peanut Research Lab in Dawson, GA entitled, Silencing of Aflatoxin Synthesis through RNA Interfence (RNAi) in Peanut Plants. The project has two main objectives, to study the genetic diversity of Aspergillus species collected in PMIL target countries and the USA, and to develop peanut lines containing RNAi gene constructs to reduce aflatoxin contamination by Aspergillus flavus infection. The data were produced as part of the first objective on genetic diversity. The genomes are available at National Center for Biotechnology Information (NCBI) database found at the landing page identified below using the numbers listed in the reference AccessionNumbers.txt.
ASCIIdata Spectra in ASCII text files, including separate files with wavelength and bandpass (FWHM) values
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ASCIIdata Files containing spectral data in ASCII text format: - measured spectra (ASCIIdata_splib07a.zip), including wavelength positions and bandpass (Full-Width at Half-Maximum; FWHM) values of channels in the spectrometers utilized - spectra interpolated to a higher number of more finely-spaced channels (ASCIIdata_splib07b.zip) - spectra convolved to other spectrometers, including the wavelength positions and bandpass (FWHM) values of the channels in the spectrometers, for example * Analytical Spectral Devices standard resolution (ASCIIdata_splib07b_cvASD.zip) * AVIRIS-Classic 2014 characteristics (ASCIIdata_splib07b_cvAVIRISc2014.zip) * Hyperspectral Mapper 2014 characteristics (ASCIIdata_splib07b_cvHYMAP2014.zip) * and others - spectra resampled to multispectral sensors, including channel wavelength positions and sensor response functions of the sensors, for example: * ASTER (ASCIIdata_splib07b_rsASTER.zip) * and others NOTE: within each zip file the ASCII data files are organized in chapter sub-folders: - ChapterM_Minerals - ChapterS_SoilsAndMixtures - ChapterC_Coatings - ChapterL_Liquids - ChapterO_OrganicCompounds - ChapterA_ArtificialMaterials - ChapterV_Vegetation GENERAL LIBRARY DESCRIPTION This data release provides the U.S. Geological Survey (USGS) Spectral Library Version 7 and all related documents. The library contains spectra measured with laboratory, field, and airborne spectrometers. The instruments used cover wavelengths from the ultraviolet to the far infrared (0.2 to 200 microns). Laboratory samples of specific minerals, plants, chemical compounds, and man-made materials were measured. In many cases, samples were purified, so that unique spectral features of a material can be related to its chemical structure. These spectro-chemical links are important for interpreting remotely sensed data collected in the field or from an aircraft or spacecraft. This library also contains physically-constructed as well as mathematically-computed mixtures. Measurements of rocks, soils, and natural mixtures of minerals have also been made with laboratory and field spectrometers. Spectra of plant components and vegetation plots, comprising many plant types and species with varying backgrounds, are also in this library. Measurements by airborne spectrometers are included for forested vegetation plots, in which the trees are too tall for measurement by a field spectrometer. The related U.S. Geological Survey Data Series publication, "USGS Spectral Library Version 7", describes the instruments used, metadata descriptions of spectra and samples, and possible artifacts in the spectral measurements (Kokaly and others, 2017). Four different spectrometer types were used to measure spectra in the library: (1) Beckman™ 5270 covering the spectral range 0.2 to 3 µm, (2) standard, high resolution (hi-res), and high-resolution Next Generation (hi-resNG) models of ASD field portable spectrometers covering the range from 0.35 to 2.5 µm, (3) Nicolet™ Fourier Transform Infra-Red (FTIR) interferometer spectrometers covering the range from about 1.12 to 216 µm, and (4) the NASA Airborne Visible/Infra-Red Imaging Spectrometer AVIRIS, covering the range 0.37 to 2.5 µm. Two fundamental spectrometer characteristics significant for interpreting and utilizing spectral measurements are sampling position (the wavelength position of each spectrometer channel) and bandpass (a parameter describing the wavelength interval over which each channel in a spectrometer is sensitive). Bandpass is typically reported as the Full Width at Half Maximum (FWHM) response at each channel (in wavelength units, for example nm or micron). The linked publication (Kokaly and others, 2017), includes a comparison plot of the various spectrometers used to measure the data in this release. Data for the sampling positions and the bandpass values (for each channel in the spectrometers) are included in this data release. These data are in the SPECPR files, as separate data
Microbial Observatory (ISS-MO): Draft Genome Sequence of two Aspergillus fumigatus Strains Isolated from the International Space Station
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Aspergillus fumigatus is a saprophytic filamentous fungus that is ubiquitous outdoors (soil decaying vegetation) and indoors (hospitals simulated closed habitats etc.). A. fumigatus can adapt to various environmental conditions and form airborne conidia that are the inoculum for a variety of diseases (e.g. non- and invasive pulmonary infections allergic bronchopulmonary aspergillosis etc.) in immunocompromised hosts. In an on-going Microbial Observatory Experiments on the International Space Station (ISS) molecular phylogeny of several fungal isolates were characterized. Two strains ISSF 21 and IF1SW-F4 were isolated from the HEPA filter and the surface of the Cupola of the ISS respectively. Using primers targeting the internal transcribed spacers ITS1 and 2 both isolates were identified as A. fumigatus. The whole genome sequence analysis of ISSF 21 revealed increased number of single nucleotide polymorphisms (SNPs) when compared to the reference A. fumigatus 293. Knowing that A. fumigatus is an opportunistic pathogen and microgravity highly influences the antibiotic susceptibility and pathogenicity of microorganisms we examined pathogenicity of both ISS isolates using the zebrafish larval model. The space station isolates (ISSF-021 and IF1SW-F4) were more virulent than two clinical strains (Af293 and CEA10) whose pathogenicity was highly characterized. Here the whole genome sequences of ISSF-021 strain are being deposited.
Genetic analyses of Astragalus sect. Humillimi data in the southwest USA
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These data were compiled to support effective conservation for species within Astragalus sect. Humillimi. Two species, A. cremnophylax var. cremnophylax and A. humillimus are federally listed as endangered taxa. The data provided herein were used to resolve the relationaships among taxa, the population structure within taxa, and genetic diversity within taxa. The data are provided in a STRUCTURE-formatted file that includes taxa, individuals, sampling localities, and the genotype scores per individual for 690 Amplified Fragment Length Polymorphisms (AFLPs).