Lack of reproduction has been hypothesized to be a factor in the decline of the pallid sturgeon (Scaphirhynchus albus) through much of its range. Spawning habitat and behavior have only recently been documented on the Lower Missouri River, which comprises over 1,300 river kilometers of undammed river. This data consists of present habitat measurements for ten pallid sturgeon spawning events from 2008-2013 that occurred in discrete reaches dispersed over hundreds of kilometers on the Lower Missouri River.

Lack of reproduction has been hypothesized to be a factor in the decline of the pallid sturgeon (Scaphirhynchus albus) through much of its range. Spawning habitat and behavior have only recently been documented on the Lower Missouri River, which comprises over 1,300 river kilometers of undammed river. This data consists of present habitat measurements for ten pallid sturgeon spawning events from 2008-2013 that occurred in discrete reaches dispersed over hundreds of kilometers on the Lower Missouri River.

The data set contains sampling and catch data associated with an experiment to examine dispersal and short-term survival of endangered pallid sturgeon (Scaphirhynchus albus) free embryos in the Missouri River of eastern Montana. The experiment was conducted as part of a flow test from Fort Peck Dam, when warm surface water from Fort Peck reservoir was released over the spillway to improve environmental conditions for pallid sturgeon. On 20240626, an estimated 354,000 1-day post-hatch (dph) pallid sturgeon free embryos produced from hatchery broodstock were released in the warm plume of the spillway, and recaptured at sites 3.9- and 8.4-miles downstream. The free embryos were sampled using multiple boats and crews at the sites, where sampling for the free embryos was conducted adjacent to the riverbed (bottom samples) and in the middle of the water column (mid-water samples). The data set includes sample site location information; site-, boat-, and time-specific catches for the released pallid sturgeon free embryos; length estimates for the free embryos; and parental crosses used to produce the free embryos.

Endangered pallid sturgeon (Scaphirhynchus albus) spawning habitats were mapped on the Tongue River near Miles, City Montana June in 2023 and 2024. Data quantify the physical characteristics of the river channel and flow; depth, velocity and habitat assessments using single-beam sonar, acoustic Doppler current profilers (ADCPs) in 2023 and single-beam sonar in 2024. Real-time kinematic Global Navigation Satellite Systems (GNSS) through a base station were used for positioning in 2023 and a Real-time GNSS correction network was used in 2024. In 2023 depths and velocities were mapped in a 1.6 kilometer long reach used by pallid sturgeon with transects that had a 15-meter spacing. In 2024 we surveyed a longitudinal profile of depth, water surface elevation, and bed elevation for 30.3 kilometers from just below Twelve Mile Dam to just upstream from the confluence with the Yellowstone River. Elevation control in 2024 used a real time virtual reference station correction subscription that utilized satellite-based elevation corrections.This dataset includes: Maps of depth and velocity for a 1.6 km long reach in 2023, raw ADCP points used to generate depth and velocity maps, raw ASCII ADCP data from transects used to generate depth and velocity maps, and a longitudinal bed and water surface profile of a 30.3 km long reach surveyed on 6/29/2024.

Layer that includes the known information on lake sturgeon breeding, feeding and concentration areas in the St. Lawrence River and Estuary according to a literature review of documents produced between 1976 and 2002. Additional Information Lake sturgeon's breeding, feeding and concentration areas were produced according to a literature review of the following documents: Blais, J.-P. et V. Legendre. 1976. La ouananiche, Salmo salar, du lac Tremblant, Québec. Québec, Ministère du Loisir, de la Chasse et de la Pêche, Direction régionale de Montréal, Rapp. tech. 06-11, 116 p. Bouthillier, L., P. Dumont et G. Roy. 1993. Répertoire des habitats du poisson de la région de Montréal, Québec. Ministère du Loisir, de la Chasse et de la Pêche du Québec, Direction régionale de Montréal. ii + 6 p. + fiches d'habitats + 18 cartes d'habitats à l'échelle 1:50 000 + bibliographie 15 p. Desjardins, S. et P. Dumont. 1990. Avis du ministère du Loisir, de la Chasse et de la Pêche au sujet de l'acceptabilité environnementale du projet Soligaz. Québec, Ministère du Loisir, de la Chasse et de la Pêche, Direction régionale de Montréal. Dimension Environnement SNC. 1990. Évaluation de l'abondance de l'Esturgeon jaune (Acipenser fulvescens) dans la baie du Cap St-Michel. Rapport complémentaire présenté au ministère de l'Environnement du Québec dans le cadre de l'étude d'impact sur l'environnement du projet Soligaz, Montréal, 24 p. Dumont, P. et R. Fortin. 1977. Relations entre les niveaux d'eau printaniers et la reproduction du Grand Brochet du Haut-Richelieu et de la baie Missisquoi. Université du Québec à Montréal, Département des sciences biologiques, pour le Bureau international Champlain Richelieu, Comité d'impact sur l'environnement, x + 108 p. Dumont, P., J. Leclerc et L. Bouthillier. 1989b. Données d'inventaire, marais Fraser, lac Saint-François, Cazaville. Été 1989. Québec, Ministère du Loisir, de la Chasse et de la Pêche, Service de l'aménage¬ment et de l'exploitation de la faune, Montréal. [Données non publiées]. Dumont, P., J. Leclerc et Y. Chagnon. 1990. Pêche électrique sur la rivière Yamaska le 18 avril 1990. Québec, Ministère du Loisir de la Chasse et de la Pêche, Service de l'aménagement et de l'exploitation de la faune, Montréal. [Données non publiées]. Enquête auprès des pêcheurs et agents du MEF et du MPO. 1995. Fournier, P. et L.-M. Soyez. 1988. Étude de l'utilisation faunique printanière du marais de Rosemère. Québec, Ministre du Loisir, de la Chasse et de la Pêche, Service de l'aménagement et de l'exploitation de la faune, Montréal, 13 p. Gendron, M. 1986. Rivière-des-Prairies. Aménagement d'un haut-fond, printemps 1986. Groupe de recherche SEEEQ ltée pour la Direction Environnement d'Hydro-Québec, 103 p. Gendron, M. 1987. Rivière-des-Prairies. Suivi de l'aménagement d'un haut-fond, printemps 1987. Le Groupe de recherche SEEEQ ltée pour la Direction Environnement d'Hydro-Québec, 60 p. Gendron, M. 1988. Rivière-des-Prairies. Suivi de l'aménagement du haut-fond, synthèse 1982-1988. Le Groupe de recherche SEEEQ ltée pour le Service de recherche en environnement et santé publique, Vice-Présidence Environnement, Hydro-Québec, 95 p. LaHaye, M. et R. Fortin. 1990. Caractérisation de l'habitat de fraie et de l'habitat des juvéniles de l'Esturgeon jaune (Acipenser fulvescens) dans la région de Montréal. Rapport de recherche préparé pour le Département des sciences biologiques de l'Université du Québec à Montréal, 81 p. + annexes. Letendre, M., B. Dumas et M. Beaudoin. 1990. Inventaire de la rivière des Prairies, au niveau de l'île de Pierre. Québec, Ministère du Loisir de la Chasse et de la Pêche, Service de l'aménagement et de l'exploitation de la faune, Montréal. [Travaux en cours]. Société de la Faune et des Parcs du Québec. 2000. Atlas des habitats critiques connus ou d'intérêt particulier pour les poissons du fleuve Saint-Laurent entre le port de Montréal et l'Île aux Coudres. Direction du développement de la faune. Therrien, J.,

The data set contains sampling data, catch data, and biological data associated with a broad-scale experiment to examine free embryo dispersal and larval settlement of endangered pallid sturgeon (Scaphirhynchus albus) in the Missouri River of eastern Montana and western North Dakota. On 20190701, two age groups of pallid sturgeon including 771,707 1-day post-hatch (dph) free embryos and 200,786 5-dph free embryos were released in the Missouri River where dispersal in the water column was assessed through 150 miles. Following dispersal, beam trawl sampling of benthic habitats was conducted to verify settlement and quantify the spatial extent of settlement. Trawling was conducted for nine consecutive weeks from 20190716 to 20190911 in eight river bends positioned between river miles 1552.0 and 1591.8. A tissue sample was obtained from captured larval Scaphirhynchus. The tissue sample was used in genetic analysis to identify species and identify parentage. The data set includes sampling location information, benthic trawl catch data for Scaphirhynchus species and other species, genetic results distinguishing Scaphirhynchus species as shovelnose sturgeon or pallid sturgeon, parentage for the pallid sturgeon, and biological data for all species.

The data set contains sampling data, catch data, and biological data associated with a broad-scale experiment to examine free embryo dispersal and larval settlement of endangered pallid sturgeon (Scaphirhynchus albus) in the Missouri River of eastern Montana and western North Dakota. On 20190701, two age groups of pallid sturgeon including 771,707 1-day post-hatch (dph) free embryos and 200,786 5-dph free embryos were released in the Missouri River where dispersal in the water column was assessed through 150 miles. Following dispersal, beam trawl sampling of benthic habitats was conducted to verify settlement and quantify the spatial extent of settlement. Trawling was conducted for nine consecutive weeks from 20190716 to 20190911 in eight river bends positioned between river miles 1552.0 and 1591.8. A tissue sample was obtained from captured larval Scaphirhynchus. The tissue sample was used in genetic analysis to identify species and identify parentage. The data set includes sampling location information, benthic trawl catch data for Scaphirhynchus species and other species, genetic results distinguishing Scaphirhynchus species as shovelnose sturgeon or pallid sturgeon, parentage for the pallid sturgeon, and biological data for all species.

Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.

Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.

Data consist of flow cytometry files (.FCS) that were generated in the analysis of larval pallid sturgeon specimens. Nuclei from produced larval sturgeon somatic cells were analyzed using flow cytometry to estimate genome size and assign ploidy. Freshly collected rainbow trout (Oncorhynchus mykiss), largemouth bass (Micropterus salmoides), or pallid sturgeon blood were used as external controls, whereby the fish blood samples were analyzed in separate tubes from the cells of prepared sturgeon larvae. All samples at 1 x 10^6 cells/mL were incubated in a propidium iodide solution to stain DNA, then analyzed by flow cytometry. Analysis software generated histograms from the experimental samples and the control standard samples. The histogram peak channels (modes) were then compared to generate a genome size estimate and additionally to visualize peaks for ploidy categorization.