180626 Thyroid Hormone PFOA PFOS Manuscript Data. This dataset is associated with the following publication: Selano, J., V. Richardson, J. Washington, and C. Mazur. Characterization of non-radiolabeled Thyroxine (T4) uptake in cryopreserved rat hepatocyte suspensions: Pharmacokinetic implications for PFOA and PFOS chemical exposure. TOXICOLOGY IN VITRO. Elsevier Science Ltd, New York, NY, USA, 58: 230-238, (2019).

The supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/toxics11120951/s1. Text S1: Chemicals; Text S2: Thyroid Hormone Chemicals and Analysis; Text S3: In Vivo Statistics; Text S4: Plasma Dosimetry Chemicals, Materials, and Analysis; Text S5: Normalization of Dosimetry Data Calculations; Text S6: Liver Dosimetry Chemicals, Materials, and Analysis; Text S7: Liver-to-Plasma Partitioning Calculations; Text S8: Non-Targeted Analysis Method and Data Processing; Text S9: Hepatocyte Metabolic Stability Assay Materials, Chemicals, and Calculations; Text S10: Plasma Protein Binding Materials, Chemicals, Assay Design, and Calculations; Text S11: In Vitro–In Vivo Extrapolation (IVIVE) Calculations. Table S1: Data processing parameters used with Sciex OS 3.0; Table S2: Data processing parameters used with Sciex MarkerView 1.3.1; Table S3: Chemical identification, vendor, purity, and experiment usage for all analytes and internal standards; Table S4: Mobile phase gradient for targeted analysis of thyroid hormones in plasma on a Sciex 6500+ QTRAP. Both mobile phases contained 0.1% formic acid as an additive; Table S5: Various instrument conditions for plasma thyroid hormone quantitation on a Sciex 6500+ QTRAP; Table S6: Monitored transitions for analysis of thyroid hormones and 13C-labeled internal standards on a Sciex 6500+ QTRAP. All ions were acquired in positive ion mode; Table S7: Mobile phase gradient for targeted analysis of HFPO-TeA on a Sciex X500R QTOF/MS. Both mobile phases contained ammonium formate (4 mM) as an additive; Table S8: Various instrument conditions for sample analysis on a Sciex X500R QTOF/MS; Table S9: Monitored transitions for analysis of HFPO-TeA using PFHxDA as an internal standard on a Sciex X500R QTOF/MS. The ion of m/z 350.97 is the in-source fragment formed from the HFPO-TeA molecular ion of m/z 660.97. All ions were acquired in negative ion mode; Table S10: Mobile phase gradient for non-targeted analysis on a Sciex X500R QTOF/MS. Ammonium formate (4 mM) was present in both mobile phases as an additive; Table S11: Mobile phase gradient for targeted analysis of HFPO-TeA on a Waters Xevo-TQS. Both mobile phases contained the additive ammonium acetate (2.5 mM); Table S12: Various instrument conditions for hepatocyte clearance and protein plasma binding assays on a Waters Xevo-TQS; Table S13: Monitored transitions for analysis of the in vitro analytes using a Waters Xevo-TQS; Table S14: Individual body weights, absolute liver weights, and relative liver weights for all rats after 5 days of exposure; Table S15: Individual concentrations for plasma T3, rT3, and T4 in all rats after 5 days of exposure to HFPO-TeA. N/A = Calculation not completed due the majority of samples being below the LOQ; Table S16: Individual HFPO-TeA plasma and plasma extract concentrations for all rats after 2 h of exposure... This dataset is associated with the following publication: Renyer, A., K. Ravindra, B. Wetmore, J. Ford, M. Devito, M. Hughes, L. Wehmas, and D. Macmillan. Dose Response, Dosimetric, and Metabolic Evaluations of Replacement PFAS Perfluoro-(2,5,8-trimethyl-3,6,9-trioxadodecanoic) Acid (HFPO-TeA). Toxics. MDPI, Basel, SWITZERLAND, 11(12): 951, (2023).

Raw data to accompanying manuscript "The Pollutant Perfluorohexane Sulfonate (PFHxS) Reduces Serum Thyroxine but Does Not Alter Thyroid Hormone Action in the Postnatal Rat Brain". This dataset is associated with the following publication: O'Shaughnessy, K., K. Bell, A. Sasser, M. Gilbert, C. Riutta, J. Ford, J. McCord, and C. Wood. The Pollutant Perfluorohexane Sulfonate (PFHxS) Reduces Serum Thyroxine but Does Not Alter Thyroid Hormone Action in the Postnatal Rat Brain. ENVIRONMENT INTERNATIONAL. Elsevier B.V., Amsterdam, NETHERLANDS, 190: 108838, (2024).

File contains data on body, liver, thyroid weights from dams and exposed offspring, serum thyroid hormones, gland and cortical gene expression, results of cognitive function tests. This dataset is associated with the following publication: Ramhoj, L., U. Haas, M. Gilbert, C. Wood, T. Svingen, D. Usai, A.M. Vinggaard, K. Mandrup, and M. Axelstad. Evaluating thyroid hormone disruption: Investigations of long-term neurodevelopmental effects in rats after perinatal exposure to perfluorohexane sulfonate (PFHxS). Scientific Reports. Nature Publishing Group, London, UK, 10(1): 2672, (2020).

Results of single concentration and concentration response testing of 165 PFAS samples from the USEPA ToxCast chemical library in nine (9) thyroid relevant assays. Chemicals were first screened in single concentration ('Single Concentration' tab) and then those chemicals with 50% or greater activity were further tested in concentration-response mode ('Concentration Response' tab). This dataset is associated with the following publication: Degitz, S., J. Olker, J. Denny, P. Degoey, P. Hartig, M. Cardon, S. Eytcheson, J. Haselman, S. Mayasich, and M. Hornung. In vitro screening of per- and polyfluorinated substances (PFAS) for interference with seven thyroid hormone system targets across nine assays. TOXICOLOGY IN VITRO. Elsevier Science Ltd, New York, NY, USA, 95: 105762, (2024).

This data set is a dose response analysis of two thyroid hormone synthesis disruptors in adult male rats. Data included serum and thyroid gland concentrations of the two chemicals tested, propylthiouracil and methimazole, as well as serum and thyroid gland hormone concentrations. These data were critical for developing a model linking highthroughput assay data on synthesis inhibition to make predictions of thyroid hormone in serum. This dataset is associated with the following publication: Hassan, I., H. El-Masri, J. Ford, A. Brennan, S. Handa, K. Friedman, and M. Gilbert. Extrapolating In Vitro and Ex Vivo Screening Assay Data for Thyroperoxidase Inhibition to Predict Serum Thyroid Hormones in the Rat. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 173(2): 280-292, (2020).

How to access data for "Decrane R, Stoker T, Murr A, Ford J, El-Masri H. Cross species extrapolation of the disruption of thyroid hormone synthesis by oxyfluorfen using in vitro data, physiologically based pharmacokinetic (PBPK), and thyroid hormone kinetics models. Curr Res Toxicol. 2023 Nov 23;5:100138. doi: 10.1016/j.crtox.2023.100138. PMID: 38074188; PMCID: PMC10697989.". This dataset is not publicly accessible because: N/A. It can be accessed through the following means: Data will be made available on request from Hisham El-Masri (el-masri.hisham@epa.gov). Format: N/A. This dataset is associated with the following publication: Decrane, R., T. Stoker, A. Murr, J. Ford, and H. El-Masri. Cross species extrapolation of the disruption of thyroid hormone synthesis by oxyfluorfen using in vitro data, physiologically based pharmacokinetic (PBPK), and thyroid hormone kinetics models. Current Research in Toxicology. Elsevier B.V., Amsterdam, NETHERLANDS, 5: 100138, (2023).

Dataset for Foley et al. 'Technical evaluation and standardization of the human thyroid microtissue assay'; Toxicological Sciences, Vol 199, Issue 1, pg 89-107, kfae014 May 2024. DOI https://doi.org/10.1093/toxsci/kfae014. This dataset is associated with the following publication: Foley, B., K. Breaux, J. Gamble, S. Lynn, R. Thomas, and C. Deisenroth. Technical Evaluation and Standardization of the Human Thyroid Microtissue Assay. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 199(1): 89-107, (2024).

This file contains summary data of thyroid hormones in serum and brain in rat dams and their pups following maternal exposure to a perflorinated substance, PFHxS and an antimicrobial, Triclosan. Gene expression in thyroid glands and liver and brain were investigated. Anatomical and bahavioral indices of developmental neurotoxicity were assessed. Result so fall of these inquiries are summarized in these datasets. This dataset is associated with the following publication: Gilbert, M.E., K. OShaughnessy, S. Thomas, C. Riutta, C. Wood, A. Smith, W. Oshiro, J. Ford, A. Hotchkiss, I. Hassan, and R.L. Ford. Thyroid Disruptors: Extrathyroidal Sites of Chemical Action and Neurodevelopmental Outcome-An Examination Using Triclosan and Perfluorohexane Sulfonate. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 183(6): 195-213, (2021).

This file contains summary data of thyroid hormones in serum and brain in rat dams and their pups following maternal exposure to a perflorinated substance, PFHxS and an antimicrobial, Triclosan. Gene expression in thyroid glands and liver and brain were investigated. Anatomical and bahavioral indices of developmental neurotoxicity were assessed. Result so fall of these inquiries are summarized in these datasets. This dataset is associated with the following publication: Gilbert, M.E., K. OShaughnessy, S. Thomas, C. Riutta, C. Wood, A. Smith, W. Oshiro, J. Ford, A. Hotchkiss, I. Hassan, and R.L. Ford. Thyroid Disruptors: Extrathyroidal Sites of Chemical Action and Neurodevelopmental Outcome-An Examination Using Triclosan and Perfluorohexane Sulfonate. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 183(6): 195-213, (2021).