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BBGD454: an Online Database for Blueberry Genomic Data Transcriptome analysis of Blueberry using 454 EST sequencing
,NOTE: This dataset is no longer publicly available.,This database houses over 500,000 sequences that were generated and assembled into approximately 15,000 contigs, annotated and functionally mapped to Gene Ontology (GO) terms.,Blueberry (Vaccinium corymbosum) is a major berry crop in the United States. Next generation sequencing methodologies, such as 454, have been demonstrated to be successful and efficient in producing a snap-shot of transcriptional activities during an organism’s developmental stage(s) or its response to biotic or abiotic stresses. Such application of this new sequencing technique allows for high-throughput, genome-wide experimental verification of known and novel transcripts. We have applied a high-throughput pyrosequencing technology (454 EST sequencing) for transcriptome profiling of blueberry during different stages of fruit development to gain an understanding of the genes that are up or down regulated during this process. We have also sequenced flower buds at four different stages of cold acclimation to gain a better understanding of the genes and biochemical pathways that are up- or down-regulated during cold acclimation, since extreme low temperatures are known to reduce crop yield and cause major losses to US farmers. We have also sequenced a leaf sample to compare its transcriptome profile with that of bud and fruit samples. Over 500,000 sequences were generated and assembled into approximately 15,000 contigs and were annotated and functionally mapped to Gene Ontology (GO) terms. A database was developed to house these sequences and their annotations. A web based interface was also developed to allow collaborators to search\browse the data and aid in the analysis and interpretation of the data. The availability of these sequences will allow for future advances, such as the development of a blueberry microarray to study gene expression, and will aid in the blueberry genome sequencing effort that is underway. This work was supported by grant 2008-51180-04861 from the USDA - Cooperative State Research, Education, and Extension Service (CSREES) Specialty Crop Research Initiative program.,
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BBGD: an Online Database for Blueberry Genomic Data
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,NOTE: This dataset is no longer publicly available.,BBGD (http://bioinformatics.towson.edu/BBGD/) was developed as a database for blueberry genomics. BBGD is both a sequence and gene expression database. It stores both EST and microarray data and allows scientists to correlate expression profiles with gene function. BBGD is a public online database. "Presently, the main focus of the database is the identification of genes in blueberry that are significantly induced or suppressed after low temperature exposure. ",To gain a better understanding of changes in gene expression associated with cold acclimation in blueberry, the Rowland laboratory (USDA-ARS, Beltsville, MD) has undertaken a genomics approach based on the analysis of Expressed Sequence Tags (ESTs). Initially, two standard cDNA libraries were constructed using RNA from cold-acclimated and non-acclimated floral buds of the blueberry cultivar ‘Bluecrop’ (Vaccinium corymbosum L.) and about 1200 5’-end ESTs were generated from each of the libraries. About 100 3’-end ESTs were generated from the cold-acclimated library as well.,The Blueberry EST database contains EST sequences from a number blueberry libraries including cold acclimated and non-acclimated libraries. It also includes forward and reverse subtractive libraries.,You can query the sequence database by clone ID, accession number or gene (clone) name below. Or you can get a list (in tabular) format of all the clones in a particular library by clicking on the library name on the left side navigation bar.,Attribution for photo: D2601-1 - Blueberry plant: Copyright free, public domain photo by Mark Ehlenfeldt,
RefSeq: NCBI Reference Sequence Database
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A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
USDA Public Citrus Genome Database
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,This is a public resource highlighting efforts at ARS in developing genome information for the Citrus Carrizo Genome. Updates and progress are reported here.,,
CottonGen Breeding Information Management System (BIMS)
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,BIMS, the Breeding Information Management System, is a secure and comprehensive online breeding management system developed for the generic Tripal Database Platform which allows breeders to store, manage, archive and analyze their private breeding program. Breeders can load data in templates provided as well as output from the Field Book App, an android app for collecting phenotype data. In addition to the private breeders BIMS, users without accounts can also view the publicly available breeding data. The fully developed version will allow users to:,,
Nucleotide
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The Nucleotide database is a collection of sequences from several sources, including GenBank, RefSeq, TPA and PDB. Genome, gene and transcript sequence data provide the foundation for biomedical research and discovery.
NCBI Datasets
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NCBI Datasets is one-stop shop for finding, browsing, and downloading genomic data. Find and download taxonomy, genome, gene, transcript, protein data, including installation of NCBI Datasets command-line tools.
Oncopeltus fasciatus genome annotations v0.5.3
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,The Oncopeltus fasciatus genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The O. fasciatus research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.1.,Oncopeltus fasciatus has been an established lab organism for over 60 years, and has been used for a wide range of studies from physiology to development and evolution. As a relatively conservative and generalized species, it affords a baseline against which other species can be compared.,For example, this species has the same piercing and sucking type mouthparts as its less benign relatives, including the blood-sucking kissing bug, Rhodnius prolixus, and the brown marmorated stink bug, Halyomorpha halys, which are disease vector and agricultural pest species, respectively. Unlike the pest species, the benign, seed-feeding Oncopeltus can be functionally investigated in the lab by RNA interference (RNAi). Comparing the genomes, and conducting experimental lab work in Oncopeltus, will help to identify unique features of the pest species, and thus inform management strategies for them.,More generally, Oncopeltus is a key species for comparisons across the insects. It is one of the few experimentally tractable hemimetabolous species that can ground comparisons with the completely metamorphosing species of the Holometabola (e.g., flies, beetles, wasps). Topics investigated in this framework include reproductive biology and development of the legs, wings, body segments, extraembryonic membranes, and overall establishment of the body plan.,This dataset presents the Oncopeltus fasciatus gene set BCM_v_0.5.3, which was generated computationally. RNA-Seq data was used with additional protein homology data for a MAKER automated annotation of the Oncopeltus fasciatus genome assembly 1.0. Further annotation method details will be available in a forthcoming publication.,NOTE: This gene set is an unstable pre-release (v0.5.3), and was provided to facilitate manual curation and analyses before the official gene set is released. Gene identifiers from this gene set will likely not be maintained.,If you wish to use this dataset, please follow the Baylor College of Medicine's conditions for data use: https://www.hgsc.bcm.edu/bcm-hgsc-conditions-use,
SoyBase and the Soybean Breeder's Toolbox
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,SoyBase is a repository for genetics, genomics and related data resources for soybean. It contains current genetic, physical and genomic sequence maps integrated with qualitative and quantitative traits.,SoyBase database was established in the 1990s as the USDA Soybean Genetics Database. Originally, it contained only genetic information about soybeans such as genetic maps and information about the Mendelian genetics of soybean. In time SoyBase was expanded to include molecular data regarding soybean genes and sequences as they became available. In 2010, the soybean genome sequence was published and it and supporting gene sequences have been integrated into the SoyBase sequence browser. SoyBase genetic maps were used in the assembly of both the Williams 82 2010 assembly (Wm82.a1.v1) and the newest genome assembly (Wm82.a2.v1).,SoyBase also incorporates information about mutant and other soybean genetic stocks and serves as a contact point for ordering strains from those populations. As association analyses continue due to various re-sequencing efforts SoyBase will also incorporate those data into the soybean genome browser as they become available. Gene expression patterns are also available at SoyBase through the SoyBase expression pages and the Soybean Gene Atlas. Other expression/transcriptome/methylomic data sets also have been and continue to be incorporated into the SoyBase genome browser.,Project No:3625-21000-062-00D Accession No: 0425040,
Database of Genotype and Phenotype (dbGaP)
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Database of Genotype and Phenotype (dbGaP) was developed to archive and distribute the data and results from studies that have investigated the interaction of genotype and phenotype in Humans.