we hypothesized that ozone-adaptation is linked to diminution of neuroendocrine stress-axes activation and glucocorticoid levels. Male Wistar-Kyoto-rats (12-week-old) were injected with vehicle or a therapeutically-relevant dexamethasone dose (0.01-mg/kg/day; intraperitoneal) for 1-month to determine if suppression of glucocorticoid signaling was linked to adaptation. Vehicle- and dexamethasone-treated rats were exposed to air or 0.8-ppm ozone, 4 hours/dayx2 or 4 days to assess the impacts of acute exposure and adaptation, respectively. This dataset is associated with the following publication: Heinriquez, A., S. Snow, J. Dye, M.C. Schladweiler, D. Alewel, C. Miller, and U. Kodavanti. The contribution of the neuroendocrine system to adaption after repeated daily ozone exposure in rats. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 447(116085): 1, (2022).

This study was performed to determine the role of adrenal-derived stress hormones in mediating brain effects after ozone exposure. Sham and adrenalectomized rats were exposed to air or ozone for 4 hours and within 2 hours after exposure brain regions, hypothalamus and brainstem were isolated and circulating pituitary hormones were analyzed. Global gene expression was analysed in these two brain regions using Illumina mRNA sequencing platform and data were analyzed using various bioinformatics platforms. The resulting data are included in this manuscript. This dataset is associated with the following publication: Henriquez, A., J. House, S. Snow, C. Miller, M. Schladweiler, A. Astriab Fisher, H. Ren, M. Valdez, P. Kodavanti, and U. Kodavanti. Ozone-induced dysregulation of neuroendocrine axes requires adrenal-derived stress hormones. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 172(1): 38-50, (2019).

This study was performed to determine the role of adrenal-derived stress hormones in mediating brain effects after ozone exposure. Sham and adrenalectomized rats were exposed to air or ozone for 4 hours and within 2 hours after exposure brain regions, hypothalamus and brainstem were isolated and circulating pituitary hormones were analyzed. Global gene expression was analysed in these two brain regions using Illumina mRNA sequencing platform and data were analyzed using various bioinformatics platforms. The resulting data are included in this manuscript. This dataset is associated with the following publication: Henriquez, A., J. House, S. Snow, C. Miller, M. Schladweiler, A. Astriab Fisher, H. Ren, M. Valdez, P. Kodavanti, and U. Kodavanti. Ozone-induced dysregulation of neuroendocrine axes requires adrenal-derived stress hormones. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 172(1): 38-50, (2019).

This dataset includes data for the manuscript in progress, Intracerebroventricular kynurenic acid modulation differentially regulates the immune and neuroendocrine stress response to acute ozone exposure. This study employed the use of kynurenic acid or the kynurenic acid synthesis inhibitor, PF-04859989 infused into the dorsal third ventricle of the brain in male WKY rats, prior to acute ozone exposure at 0.8 ppm, to evaluate the role of kynurenic acid in modulating the neuroendocrine stress response to ozone. Our results indicate that kynurenic acid may have a protective role in the brain, in attenuating the pulmonary and systemic effects of ozone exposure, perhaps through multiple mechanisms. This dataset is associated with the following publication: Rentschler, K., M.C. Schladweiler, R. Grindstaff, W. Williams, P.R. Kodavanti, D. Freeborn, L. Klein, G. Jung, D. Herr, P. Evansky, J. McKee, S. Gavett, and U. Kodavanti. Differential Effects of Intracerebroventricular Kynurenic Acid Regulation on the Inflammatory and Neuroendocrine Response to Acute Ozone Exposure. Presented at Society of Toxicology, Orlando, FL, USA, 03/16/2025 - 03/20/2025.

This data set was used for the manuscript titled "Ozone-induced Changes in Oxidative Stress Parameters in Brain regions of Adult, Middle-age, and Senescent Brown Norway Rats". It includes various biochemical endpoints and neurobehavior. This dataset is associated with the following publication: Kodavanti, P.R., M. Valdez, J. Richards, D. Agina-Obu, P. Phillips, K. Jarema, and U. Kodavanti. Ozone-induced changes in oxidative stress parameters in brain regions of adult, middle-age, and senescent Brown Norway rats. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 410: 115351, (2020).

This data set was used for the manuscript titled "Ozone-induced Changes in Oxidative Stress Parameters in Brain regions of Adult, Middle-age, and Senescent Brown Norway Rats". It includes various biochemical endpoints and neurobehavior. This dataset is associated with the following publication: Kodavanti, P.R., M. Valdez, J. Richards, D. Agina-Obu, P. Phillips, K. Jarema, and U. Kodavanti. Ozone-induced changes in oxidative stress parameters in brain regions of adult, middle-age, and senescent Brown Norway rats. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 410: 115351, (2020).

Ozone exposure induces neuroendocrine stress response, which causes lymphopenia. We hypothesized that ozone-induced increases in stress hormones will temporally follow changes in circulating granulocytes, monocytes and lymphocyte subpopulations. The goal of this study was to chronicle the changes in circulating stress hormones, cytokines, and leukocyte trafficking during 4-hour exposure to ozone. Male Wistar Kyoto rats were exposed to air or ozone (0.4 or 0.8 ppm) for 0.5, 1, 2, or 4 hours. After each time point, we assessed, circulating stress hormones and cytokines, lung gene expression, and live and apoptotic granulocytes, monocytes (classical and non-classical), and lymphocytes (B, Th and Tc) in blood, thymus and spleen using flow cytometry. This dataset is associated with the following publication: Henriquez, A., W. Williams, S. Snow, M.C. Schladweiler, C. Fisher, M. Hargrove, D. Alewel, C. Colonna, S. Gavett, C. Miller, and U. Kodavanti. The Dynamicity of Acute Ozone-Induced Systemic Leukocyte Trafficking and Adrenal-Derived Stress Hormones. TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 458(152823): 1, (2021).

Ozone exposure induces neuroendocrine stress response, which causes lymphopenia. We hypothesized that ozone-induced increases in stress hormones will temporally follow changes in circulating granulocytes, monocytes and lymphocyte subpopulations. The goal of this study was to chronicle the changes in circulating stress hormones, cytokines, and leukocyte trafficking during 4-hour exposure to ozone. Male Wistar Kyoto rats were exposed to air or ozone (0.4 or 0.8 ppm) for 0.5, 1, 2, or 4 hours. After each time point, we assessed, circulating stress hormones and cytokines, lung gene expression, and live and apoptotic granulocytes, monocytes (classical and non-classical), and lymphocytes (B, Th and Tc) in blood, thymus and spleen using flow cytometry. This dataset is associated with the following publication: Henriquez, A., W. Williams, S. Snow, M.C. Schladweiler, C. Fisher, M. Hargrove, D. Alewel, C. Colonna, S. Gavett, C. Miller, and U. Kodavanti. The Dynamicity of Acute Ozone-Induced Systemic Leukocyte Trafficking and Adrenal-Derived Stress Hormones. TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 458(152823): 1, (2021).

Dorsal third ventricle infusions of the alpha 2 adrenergic receptor antagonist, mianserin- which increases brain concentrations of dopamine, serotonin, and acetylcholine, or the glucocorticoid receptor antagonist, mifepristone, were performed in male WKY rats prior to acute ozone exposure at 0.8 ppm for 4 hours. This experiment was designed to target hippocampal and hypothalamic neuromodulation to determine the role of indoleamines, monoamines, and glucocorticoids in mediating the systemic and pulmonary effects to ozone exposure.

In this study, using male Wistar and Wistar-derived Goto-Kakizaki (GK) rats, which exhibit a non-obese type-2 diabetes phenotype, we investigated whether two key metabolic stressors, type-2 diabetes and a high-cholesterol atherogenic diet, exacerbate ozone-induced metabolic effects. Rats were fed a normal control diet (ND) or high-cholesterol diet (HCD) for 12 weeks starting at 4 week-age and then exposed to filtered air or 1.0-ppm ozone (6h/day) for 1 or 2 days. Metabolic responses were analyzed at the end of each day and after an 18-hour recovery period following the 2-day exposure. This dataset is associated with the following publication: Snow, S., A. Henriquez, A. Fisher, B. Vallanat, J. House, M.C. Schladweiler, C. Wood, and U. Kodavanti. Peripheral Metabolic Effects of Ozone Exposure in Healthy and Diabetic Rats on Normal or High-Cholesterol Diet. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 415(115427): 1, (2021).