Data were collected in association with the application of visible light in laboratory and field settings to elicit behavioral responses from the invasive virile crayfish (Faxonius virilis). Data for laboratory light trials include water temperature, dissolved oxygen, and conductivity, crayfish sex, weight and size, light lux, proportion of time spent in each zone (lit versus unlit), total distance moved, mean velocity, and acceleration. Data for pond trials include crayfish sex and size, and count of crayfish captured by lit and baited traps.

Invasive crayfish are known to displace native crayfish species, alter aquatic habitat and community structure and function, and are serious pests for fish hatcheries. White River Crawfish (WRC; Procambarus acutus) were inadvertently introduced to a warm-water fish hatchery in Missouri, USA, possibly in an incoming fish shipment. We evaluated the use of chemical control for crayfish to ensure incoming and outgoing fish shipments from hatcheries do not contain live crayfish. We conducted acute (less than or equal to 24 hr) static toxicity tests to determine potency, dose-response, and selectivity of pesticides to WRC, Virile Crayfish (VC; Orconectes virilis), and Fathead Minnow (FHM; Pimephales promelas). Data included are: Collection location and size of test organisms; Test chemical concentrations and recovery; Mortality and effect-based responses of test organisms; Water quality of test solutions

Survival, behavior, reproduction, morphometric measurements, and tissue analyses of crayfish, mussels, and fish from acute toxicity tests. Exposure and recovery water quality data are also included for quality assurance purposes.

Survival, behavior, reproduction, morphometric measurements, and tissue analyses of crayfish, mussels, and fish from acute toxicity tests. Exposure and recovery water quality data are also included for quality assurance purposes.

This data was collected to determine responses of red swamp crayfish and rusty crayfish to CO2-enriched water to determine the utility of CO2 as a crayfish control tool. Avoidance tests, emergence tests, and loss of equilibrium (LOE) tests were performed to determine crayfish behavior in CO2-enriched water. Avoidance data (TimeinChamber2.csv, Crossings.csv, FirstLastShuttle.csv, AvoidanceLengthsandWeights.csv) were collected on 20171220 through 20180214. CO2 was injected in one side of a choice chamber under light or dark conditions and 10oC or 24oC water temperature. Shuttle movements were recorded and used to determine the number of crossings from the CO2 injected side to the non-injected side, the CO2 concentration and time of the first and last shuttle after CO2 injection began, and the total times spent in the injected and non-injected chambers. Loss of equilibrium (LOE) data were collected on 20180117 through 20180119 (LOEStats.csv). Crayfish were placed in a bucket with water and CO2 was injected into the bucket until the crayfish lost equilibrium. The concentration of CO2 and time until LOE occurred was recorded along with other behaviors induced by the elevated CO2 concentration. Red swamp crayfish emergence data was collected on 20180221 through 20180227 (Incline.csv). One side of the choice chamber used in the behavior/shuttle trials was isolated and an incline ramp was placed on the tank. CO2 was then injected into the tank and the behavior of the crayfish was recorded. Behavior data was recorded as in the tank, on the incline, or out of the water.

This data was collected to determine responses of red swamp crayfish and rusty crayfish to CO2-enriched water to determine the utility of CO2 as a crayfish control tool. Avoidance tests, emergence tests, and loss of equilibrium (LOE) tests were performed to determine crayfish behavior in CO2-enriched water. Avoidance data (TimeinChamber2.csv, Crossings.csv, FirstLastShuttle.csv, AvoidanceLengthsandWeights.csv) were collected on 20171220 through 20180214. CO2 was injected in one side of a choice chamber under light or dark conditions and 10oC or 24oC water temperature. Shuttle movements were recorded and used to determine the number of crossings from the CO2 injected side to the non-injected side, the CO2 concentration and time of the first and last shuttle after CO2 injection began, and the total times spent in the injected and non-injected chambers. Loss of equilibrium (LOE) data were collected on 20180117 through 20180119 (LOEStats.csv). Crayfish were placed in a bucket with water and CO2 was injected into the bucket until the crayfish lost equilibrium. The concentration of CO2 and time until LOE occurred was recorded along with other behaviors induced by the elevated CO2 concentration. Red swamp crayfish emergence data was collected on 20180221 through 20180227 (Incline.csv). One side of the choice chamber used in the behavior/shuttle trials was isolated and an incline ramp was placed on the tank. CO2 was then injected into the tank and the behavior of the crayfish was recorded. Behavior data was recorded as in the tank, on the incline, or out of the water.

This study evaluated carbon dioxide (CO2) injected into water as a possible behavioral stimulant to enhance capture and removal of invasive red swamp crayfish (RSC, Procambarus clarkii Girard, 1852) from a retention pond in southeastern Michigan. Objectives of this study were to (1) determine if target CO2 concentrations were attainable within the infested pond, and (2) determine if CO2 treatment was effective to push RSC towards shorelines or onto dry land where they could be collected and removed. Carbon dioxide was applied directly into one treatment pond (~2,500 m3) in Novi, MI. Two nearby ponds in Livonia, MI were used as untreated control ponds. Crayfish removal efficiency was evaluated in all ponds using baited traps and shoreline surveys. Results showed that the CO2 treatment pond reached its target concentration of >200 milligrams per liter (mg/L) CO2, a benchmark determined from previous laboratory studies, approximately 11 hours after injection started and was maintained between 200-351 mg/L CO2 for approximately 2.5 days. During treatment, some emergent crayfish were observed near influent culverts around the pond, possibly indicative of a behavioral response. However, the number of individuals and crayfish observations were minimal and infrequent. Crayfish continued to be removed throughout CO2 treatment with baited traps and perimeter surveys, but differences in catch rates between the treatment and control ponds were not apparent and confounded by a temporal decline in catch rates across all ponds. Overall, this study demonstrated that open-water treatment applications with CO2 are possible, but its effectiveness to enhance RSC removal was unclear due to limited crayfish observations.

This study evaluated carbon dioxide (CO2) injected into water as a possible behavioral stimulant to enhance capture and removal of invasive red swamp crayfish (RSC, Procambarus clarkii Girard, 1852) from a retention pond in southeastern Michigan. Objectives of this study were to (1) determine if target CO2 concentrations were attainable within the infested pond, and (2) determine if CO2 treatment was effective to push RSC towards shorelines or onto dry land where they could be collected and removed. Carbon dioxide was applied directly into one treatment pond (~2,500 m3) in Novi, MI. Two nearby ponds in Livonia, MI were used as untreated control ponds. Crayfish removal efficiency was evaluated in all ponds using baited traps and shoreline surveys. Results showed that the CO2 treatment pond reached its target concentration of >200 milligrams per liter (mg/L) CO2, a benchmark determined from previous laboratory studies, approximately 11 hours after injection started and was maintained between 200-351 mg/L CO2 for approximately 2.5 days. During treatment, some emergent crayfish were observed near influent culverts around the pond, possibly indicative of a behavioral response. However, the number of individuals and crayfish observations were minimal and infrequent. Crayfish continued to be removed throughout CO2 treatment with baited traps and perimeter surveys, but differences in catch rates between the treatment and control ponds were not apparent and confounded by a temporal decline in catch rates across all ponds. Overall, this study demonstrated that open-water treatment applications with CO2 are possible, but its effectiveness to enhance RSC removal was unclear due to limited crayfish observations.

This study deployed acoustic telemetry into a red swamp crayfish-infested water retention pond (1.0 ac) near Novi, Michigan to document the movements and behavior of telemetered red swamp crayfish (Procambarus clarkii). Telemetry was setup in the pond for approximately 3 months. Spreadsheets include telemetry data with position estimates for individual crayfish.

This study evaluated the use of dissolved carbon dioxide as a control method for invasive crayfish. Five concentrations of CO2 were administered to twenty-four test tanks at a water temperature of 5°C. Initially, Red Swamp Crayfish (Procambarus clarkii) were intended to be the test species used in the study. However, problems with obtaining a healthy population of RSC occurred, so Virile Crayfish (Faxonius virilis) were used instead. These datasets include water quality, biometric, behavioral observation, and CO2 titration data from two trials.