The dataset contains the raw data used for the correlation analyses. This dataset is associated with the following publications: Schaeffer, B., J. SantoDomingo, and B. Kreakie. SSWR 4.3 Tools for HABs Risk Characterization and Assessment FY20 Report. U.S. Environmental Protection Agency, Washington, DC, USA. Chaffin, J., J. Bratton, E. Verhamme, H. Bair, A. Beecher, C. Binding, J. Birbeck, T. Bridgeman, X. Chang, J. Crossman, W. Currie, T. Davis, G. Dick, K. Drouillard, R. Errera, T. Frenken, H. MacIsaac, A. McClure, R.M. McKay, L. Reitz, J. SantoDomingo, K. Stanislaawczyk, R. Stumpf, Z. Swan, B. Synder, J. Westrick, P. Xue, C. Yancey, A. Zastepa, and X. Zhou. The Lake Erie HABs Grab: A binational collaboration to characterize the western basin cyanobacterial harmful algal blooms at an unprecedented high-resolution spatial scale. Harmful Algae. Elsevier B.V., Amsterdam, NETHERLANDS, 108: 102080, (2021).

Log10 fecal score ratios for eligible microbial source tracking qPCR assays. This dataset is associated with the following publication: Brumfield, K.D., J.A. Cotruvo, O. Shanks, M. Sivaganesan, J. Hey, N.A. Hasan, A. Huq, R.R. Colwell, and M.B. Leddy. Metagenomic Sequencing and Quantitative Real-Time PCR for Fecal Pollution Assessment in an Urban Watershed. Frontiers in Water. Frontiers, Lausanne, SWITZERLAND, 3: 626849, (2021).

The Whole Genome Shotgun project has been deposited in DDBJ/ENA/GenBank under the BioProject PRJNA487286 with the following accession numbers CP061840 (chromosome) and CP061841 (plasmid). The raw sequence reads have been submitted to the NCBI SRA under the accession numbers SRR13076822 and SRR13076823. This dataset is associated with the following publication: Gomez-Alvarez, V., L. Boczek, I. Raffenberg, and R. Revetta. Closed Genome and Plasmid Sequences of Legionella pneumophila AW-13-4, Isolated from a Hot Water Loop System of a Large Occupational Building. Microbiology Resource Announcements. American Society for Microbiology, Washington, DC, USA, 10(1): e01276-20, (2021).

Draft Genome Sequences of isolates belonging to family Methylobacteriaceae Isolated from the International Space Station

Genome sequence, PCR clone sequences and qPCR data. This dataset is associated with the following publication: Linz, D., K. McIntosh, I. Struewing, S. Klemm, B. McMinn, R. Haugland, E. Villegas, and J. Lu. Genomic Characterization and Wetland Occurrence of a Novel Campylobacter Isolate from Canada Geese. Microorganisms. MDPI, Basel, SWITZERLAND, 11(3): 648, (2023).

These data describe microbiological analyses performed on groundwater samples from domestic drinking water supply collected from 42 groundwater wells in the Central Valley of California. Samples were collected between January 2014 and April 2014 for the Groundwater Ambient Monitoring and Assessment (GAMA) program priority basin assessment of the Madera, Chowchilla, and Kings (MACK) groundwater sub-basins’ shallow aquifers. A total of 75 wells were sampled for the MACK study unit between August 2013 and April 2014. Samples for this dataset were vacuum filtered and plated on MI and mEI agars prior to incubation to promote colony growth. Colonies were tallied by their species into columns for various fecal indicator bacteria (FIBs): total coliforms (TCs), Escherichia coli (E. coli), enterococci. Non-target growths were also counted and tallied. Six additional replicate samples were collected for quality assurance. Of the 579 total FIB colonies detected, 106 were selected for polymerase chain reaction (PCR) analysis with the goal of sequencing their DNA. Selected colonies consisted of both target and non-target growths and were taken from 14 samples collected at 13 different wells. DNA sequencing was successful for 34 of the sampled colonies out of a total of 59 submitted. Results for these analyses were reported in FASTA format with the number of bases and their starting position indicated for each batch.

Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene uptake was tracked over time and reported in T1_soil_microcosm_v2.0.csv. DNA was extracted from soil microcosm samples for microbial community analysis based on 16S rRNA gene sequencing; the resulting operational taxonomic units are presented in T2_soil_OTU_v2.0.csv. Bradyrhizobium sp. strain I71 was isolated from the soil microcosms and acetylene uptake and cell growth data for the isolate over time are shown in T3_soil_isolate_v2.0.csv. Nitrogen fixation assays for the pure culture of Bradyrhizobium sp. strain I71 are reported in T4_N2_fixation_v2.0.csv. Acetylene concentrations and cell densities from acetylenotrophic and heterotrophic growth assays for Bradyrhizobium sp. strain I71 are reported in T5_GrowthCurve_v2.0.csv