This excel file contains the resultant data from a study on iodotyrosine deiodinase in the model amphibian species, Xenopus laevis. These data include: tadpole growth and development, thyroid hormones in plasma and glands, and expression of thyroid-relevant genes. The initial worksheet tab that provides metadata for each dataset included in the other worksheets that make up the file. This dataset is associated with the following publication: Olker, J., J. Haselman, P. Kosian, K. Donnay, J. Korte, C. Blanksma, M. Hornung, and S. Degitz. Evaluating iodide recycling inhibition as a novel molecular initiating event for thyroid axis disruption in amphibians. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 166(2): 318-331, (2018).

Supplementary materials for "Olker JH, Korte JJ, Haselman JT, Hornung MW, Degitz SJ. Cross-species comparison of chemical inhibition of human and Xenopus iodotyrosine deiodinase. Aquat Toxicol. 2022 Aug;249:106227. doi: 10.1016/j.aquatox.2022.106227. Epub 2022 Jun 15. PMID: 35767922; PMCID: PMC9887787." The excel spreadsheet contains the resultant data from an assay for chemical inhibition of amphibian Iodotyrosine Deiodinase (IYD) enzyme activity. 154 chemicals from the EPA’s ToxCast chemical library were tested in concentration-response and these amphibian IYD assay results were compared to those from the human IYD inhibition assay reported in Olker et al. 2021 (doi:10.1016/j.tiv.2020.105073). The same set of 154 chemicals were tested in both assays and compared here. This data set the median, minimum, and maximum inhibition produced at each concentration for each tested chemical. A model inhibitor (3-Nitro-L-Tyrosine) was included on each plate as a positive control, with concentration response data for those curves also included in the data set. This dataset is associated with the following publication: Olker, J., J. Korte, J. Haselman, M. Hornung, and S. Degitz. Xenopus laevis and Human iodotyrosine deiodinase enzyme cross-species sensitivity to inhibition by ToxCast chemicals.. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 249: N/A, (2022).

This dataset contains in vitro and in vivo biochemical and apical (i.e., growth/development) data pertaining to iodotyrosine deiodinase inhibition in the African clawed frog (Xenopus laevis). This dataset is associated with the following publication: Haselman, J., J. Olker, P. Kosian, J. Korte, J. Denny, J. Tietge, M. Hornung, and S. Degitz. Characterization of the Mechanistic Linkages Between Iodothyronine Deiodinase Inhibition and Impaired Thyroid-Mediated Growth and Development in Xenopus Laevis Using Iopanoic Acid. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 187(1): 139-149, (2022).

Dataset for "Combining in vitro and in silico New Approach Methods to investigate type 3 iodothyronine deiodinase chemical inhibition across species". This dataset is associated with the following publication: Mayasich, S., M. Goldsmith, K. Mattingly, and C. Lalone. Combining In Vitro and In Silico New Approach Methods to Investigate Type 3 Iodothyronine Deiodinase Chemical Inhibition Across Species. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 42(5): 1032-1048, (2023).

Data set includes optimized assay parameters and kinetic characterization for xenopus laevis deiodinase type 3 enzyme, and inhibition activity of 352 ToxCast chemicals against this enzyme. This dataset is associated with the following publication: Mayasich, S., J. Korte, J. Denny, P. Hartig, J. Olker, P. Degoey, J. O'Flanagan, S. Degitz, and M. Hornung. Xenopus laevis and human type 3 iodothyronine deiodinase enzyme cross-species sensitivity to inhibition by ToxCast chemicals. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 73: 105141, (2021).

Iodide uptake following a two-hour incubation with concentrations of 5-ATcompared to control iodide uptake in FRTL thyroid follicular cells. This dataset is associated with the following publication: Adams, V., M. Bazar, E. Reinke, A. Buckalew, and W. Eck. In vitro and in vivo effects of 5-Aminotetrazole (5-AT)- an energetic compound. REGULATORY TOXICOLOGY AND PHARMACOLOGY. Elsevier Science Ltd, New York, NY, USA, 111(104573): 1, (2020).

This dataset was generated to support parameterization of a larval amphibian thyroid axis computational model. Specifically, plasma iodine levels were determined not only to inform a concentration value for model parameter specification in the model, but also to support the notion that aquatic and terrestrial species should be considered substantially different with regard to circulating iodide levels. Other computational model physical parameters were informed by evaluating Xenopus laevis thyroid gland histological images for morphological features including thyroid follicular cell numbers and areas. This dataset is associated with the following publication: Haselman, J., J. Nichols, K. Mattingly, M. Hornung, and S. Degitz. A biologically based computational model for the hypothalamic-pituitary-thyroid (HPT) axis in Xenopus laevis larvae. MATHEMATICAL BIOSCIENCES. Elsevier Science Ltd, New York, NY, USA, 362: 109021, (2023).

Data pertaining to a NIS-expressing cell line, hNIS-HEK293T-EPA, and its screening capabilities for determining inhibitors of NIS-mediated iodide uptake. This dataset is associated with the following publication: Hallinger, D., A. Murr, A. Buckalew, S. Simmons, T. Stoker, and S. Laws. Development of a Screening Approach to Detect Thyroid Disrupting Chemicals that Inhibit the Human Sodium/Iodide Symporter (NIS). TOXICOLOGY IN VITRO. Elsevier Science Ltd, New York, NY, USA, 66-78, (2017).

How to access data for "Decrane R, Stoker T, Murr A, Ford J, El-Masri H. Cross species extrapolation of the disruption of thyroid hormone synthesis by oxyfluorfen using in vitro data, physiologically based pharmacokinetic (PBPK), and thyroid hormone kinetics models. Curr Res Toxicol. 2023 Nov 23;5:100138. doi: 10.1016/j.crtox.2023.100138. PMID: 38074188; PMCID: PMC10697989.". This dataset is not publicly accessible because: N/A. It can be accessed through the following means: Data will be made available on request from Hisham El-Masri (el-masri.hisham@epa.gov). Format: N/A. This dataset is associated with the following publication: Decrane, R., T. Stoker, A. Murr, J. Ford, and H. El-Masri. Cross species extrapolation of the disruption of thyroid hormone synthesis by oxyfluorfen using in vitro data, physiologically based pharmacokinetic (PBPK), and thyroid hormone kinetics models. Current Research in Toxicology. Elsevier B.V., Amsterdam, NETHERLANDS, 5: 100138, (2023).

Disruption of thyroid hormone signaling is a form of endocrine disruption that is of concern to both human health and ecosystems. Research is being conducted to define the biological targets chemicals may interact with to disrupt thyroid hormone signaling and the stages in development where that disruption can most readily lead to adverse effects. The present data characterize the expression of key genes associated with thyroid hormone signaling and regulation over the course of development for two small fish widely used in aquatic ecotoxicology research. These data provide baseline information that can lead to a more complete understanding of which thyroid disrupting chemicals fish may be susceptible to and at which stages in development. This dataset is associated with the following publication: Vergauwen, L., J. Cavallin, G. Ankley, C. Bars, I. Gabriels, E. Michiels, K. Nelson, J. Periz-Stanacev, E. Randolph, S. Robinson, T. Saari, A. Schroeder, E. Stinckens, J. Swintek, S. Van Cruchten, E. Verbueken, D. Villeneuve, and D. Knapen. Gene transcription ontogeny of thyroid-axis development in early-life stage fathead minnow and zebrafish. Journal of Experimental Biology. The Company of Biologists LIMITED, Cambridge, UK, 266: 878-1002, (2018).