The US Environmental Protection Agency conducts ecological risk assessments with a battery of fish toxicity tests that include acute, early life stage, and reproduction tests. While endpoints in these tests (survival, growth and reproduction) are conceptually related, because they are measured in separate exposures, the quantitative relationships between them are difficult to determine and largely ignored. In the current test, fathead minnows (FHM) were exposed for 28 days to 1 mg/L or 2 mg/L carbaryl, a well-studied carbamate insecticide, in early life stages and then reared in clean water until adulthood, when reproduction was assessed. Also. weekly growth measurements were taken throughout the test to determine growth rates during and after exposure. Growth curves derived from these measurements were then compared to the reproductive output. The data indicate that carbaryl reduced growth rate only for a brief time early in the exposure. However, this brief effect impacted overall growth into adulthood and lowered the reproductive output of exposed FHM. The effect of a transient exposure early in life to carbaryl could have later population-level impacts by causing mortality, lowering growth rates, and reducing reproductive output. This dataset is associated with the following publication: Flynn, K., S. Kadlec, V. Kurker, and M. Etterson. Effects of a 28-day early life stage exposure to carbaryl on fathead minnow long-term growth and reproduction. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 242: 106018, (2022).

This dataset provides exposure and effects data for two experiments regarding the dietborne toxicity of inorganic arsenic on fathead minnows survival and growth. There are separate spreadsheets for the two experiments, at the replicate exposure chamber level, of fish exposure, survival, and weights and calculated growth metrics. Each spreadsheet provides footnote descriptions of the data and calculations provided. Descriptions of experimental protocols are also provided as supporting documents. This dataset is associated with the following publication: Erickson, R., D. Mount, T. Highland, R. Hockett, D. Hoff, C. Jenson, and T. Lahren. The relative importance of waterborne and dietborne As exposure on survival and growth of juvenile fathead minnows. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 211: 18-28, (2019).

This dataset provides exposure and effects data for two experiments regarding the dietborne toxicity of inorganic arsenic on fathead minnows survival and growth. There are separate spreadsheets for the two experiments, at the replicate exposure chamber level, of fish exposure, survival, and weights and calculated growth metrics. Each spreadsheet provides footnote descriptions of the data and calculations provided. Descriptions of experimental protocols are also provided as supporting documents. This dataset is associated with the following publication: Erickson, R., D. Mount, T. Highland, R. Hockett, D. Hoff, C. Jenson, and T. Lahren. The relative importance of waterborne and dietborne As exposure on survival and growth of juvenile fathead minnows. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 211: 18-28, (2019).

Given that larval fish stages provide opportunities for development of stressor-selective screening level tests, this work aimed to investigate the optimal age of fathead minnow larva and duration of exposure for the pharmaceutical, propranolol, found in the environment. Differentially expressed genes were studied as well as gene set enrichment analysis. This dataset is associated with the following publication: Biales, A., D. Bencic, R. Flick, and G. Toth. Effects of Age and Exposure Duration on the Sensitivity of Early Life Stage Fathead Minnow (Pimephales promelas) to Waterborne Propranolol Exposure. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 43(4): 807-820, (2024).

Data are provided describing reproduction, length, weight, liver weight, and ovary weight in fish exposed to sediment spiked with weathered oil. Data are also provided on analytical chemistry of water and sediment to which fish were exposed in various treatments. This dataset is associated with the following publication: Raimondo , S., B. Hemmer , C. Lilavois , J. Krzykwa , A. Almario , J. Awkerman , and M. Barron. Effects of Louisiana crude oil on the sheepshead minnow (Cyprinodon variegatus) during a life-cycle exposure to laboratory oiled sediment. ENVIRONMENTAL TOXICOLOGY. John Wiley & Sons, Ltd., Indianapolis, IN, USA, 31(11): 1627-1639, (2016).

Data are provided describing reproduction, length, weight, liver weight, and ovary weight in fish exposed to sediment spiked with weathered oil. Data are also provided on analytical chemistry of water and sediment to which fish were exposed in various treatments. This dataset is associated with the following publication: Raimondo , S., B. Hemmer , C. Lilavois , J. Krzykwa , A. Almario , J. Awkerman , and M. Barron. Effects of Louisiana crude oil on the sheepshead minnow (Cyprinodon variegatus) during a life-cycle exposure to laboratory oiled sediment. ENVIRONMENTAL TOXICOLOGY. John Wiley & Sons, Ltd., Indianapolis, IN, USA, 31(11): 1627-1639, (2016).

Data set includes empirical results from 60 h, 10 d, and 21 d exposures of female fathead minnows to the fungicide imazalil as well as simulations from predictive models anchored to an established adverse outcome pathway (https://aopwiki.org/aops/25). Contents are organized into multiple tabs: (1) Simulated effects on plasma 17b-estradiol and vitellogenin used to inform experimental design. (2) Model simulations based on nominal concentrations used in the 60 h, 10 d, and 21 d exposures. (3) Biological effects data from the 60 h experiment. (4) Analytical exposure verification from the 60 h experiment. (5) Biological effects data from the 10 d exposure. (6) Biological effects data from 21 d exposure. (7) Analytical exposure verification from the 10 d and 21 d exposures. (8) Reproduction data from the 10 d and 21 d exposures. (9) Simulated reproduction results based on nominal exposure concentrations used in the 10 d and 21 d exposures. (10) Histopathology evaluations for selected females from the 10 d and 21 d exposures. This dataset is associated with the following publication: Villeneuve, D., B. Blackwell, C. Blanksma, J. Cavallin, W. Cheng, R. Conolly, K. Conrow, D. Feifarek, L. Heinis, K. Jensen, M. Kahl, R. Milsk, S. Poole, E. Randolph, T. Saari, K. Watanabe, and G. Ankley. Case Study in 21st-Century Ecotoxicology: Using In Vitro Aromatase Inhibition Data to Predict Reproductive Outcomes in Fish In Vivo. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 42(1): 100-116, (2023).

Data set includes empirical results from 60 h, 10 d, and 21 d exposures of female fathead minnows to the fungicide imazalil as well as simulations from predictive models anchored to an established adverse outcome pathway (https://aopwiki.org/aops/25). Contents are organized into multiple tabs: (1) Simulated effects on plasma 17b-estradiol and vitellogenin used to inform experimental design. (2) Model simulations based on nominal concentrations used in the 60 h, 10 d, and 21 d exposures. (3) Biological effects data from the 60 h experiment. (4) Analytical exposure verification from the 60 h experiment. (5) Biological effects data from the 10 d exposure. (6) Biological effects data from 21 d exposure. (7) Analytical exposure verification from the 10 d and 21 d exposures. (8) Reproduction data from the 10 d and 21 d exposures. (9) Simulated reproduction results based on nominal exposure concentrations used in the 10 d and 21 d exposures. (10) Histopathology evaluations for selected females from the 10 d and 21 d exposures. This dataset is associated with the following publication: Villeneuve, D., B. Blackwell, C. Blanksma, J. Cavallin, W. Cheng, R. Conolly, K. Conrow, D. Feifarek, L. Heinis, K. Jensen, M. Kahl, R. Milsk, S. Poole, E. Randolph, T. Saari, K. Watanabe, and G. Ankley. Case Study in 21st-Century Ecotoxicology: Using In Vitro Aromatase Inhibition Data to Predict Reproductive Outcomes in Fish In Vivo. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 42(1): 100-116, (2023).

This data set describes developmental and physiological effects observed in fathead minnow embryos that were exposed to sodium nitrate or conductivity-matched controls. Exposures were conducted in the laboratory from fertilization through 21 days post-fertilization (dpf). Nitrate doses were 0, 2, 5, 10, 25, or 100 milligrams per liter nitrate-nitrogen; conductivity controls were matched to the 10 and 100 milligrams per liter nitrate-nitrogen treatments. During the experiment, we assessed heart rate and percent arrhythmic heart beats at 3 dpf, timing of hatch and inflation of the posterior and anterior swim bladders, presence of pericardial edema or scoliosis, fish metrics at 21 dpf (fish length and weight; swim bladder length, width, and volume), and mortality. Water quality data during the exposure period are also presented, and include conductivity, pH, nitrate-nitrogen, total ammonia, unionized ammonia, and dissolved oxygen.