,This package includes the data from field experiments to measure the range of attraction of two "male lures" on two different pest fruit fly species via Mark-Release-Recapture (MRR). These values will be of importance to those seeking to optimize fruit fly detection networks or other networks of traps. Methyl eugenol is found to be more attractive to Bactrocera dorsalis compared with trimedlure to Ceratitis capitata. Data consists of number released, proportion responsive, quality control assay results, and recaptures in traps set in a grid pattern after the release.,Resources in this dataset:,,

,Study aim was to determine establishment success of antibiotic resistance bacteria in laboratory and mass-reared Mediterranean fruit fly (Ceratitis capitata). Experiments conducted in Hilo, HI, USA by the USDA ARS. Datasets include two different datatypes: 1) data from plate counts (CFUs) on nutrient-rich and antibiotic-amended data and 2) amplicon sequence variant (ASVs) count data from full-length 16S rRNA sequencing using PacBio Kinnex mas-Seq on a PacBio Revio sequencer and associated taxonomy and .fasta files. Experiments were performed with two different fly lines, one maintained by the USDA ARS and another by California Department of Agriculture (CDFA). Experiments were performed with a target Enterobacter strain that was transformed with mScarlet and antibiotic resistance selection markers. Experiments evaluated the impact of fly age and inoculation formulation (via diet) on the conditions in which bacteria would be established under different dietary conditions. Data show that liquid diets support establishment of bacteria, regardless of age class and source. Sequence data suggest additional Enterobacter strains are present in flies beyond the target strain and these can be distinguished via full-length 16S rRNA sequencing, but not by shorter amplicon fragments.,

,Mediterranean fruit fly males reared for sterile insect technique (SIT) have been shown to have differences in their microbiomes relative to other population sources, which has been postulated to be a factor in the how well flies compete with wild conspecifics. To identify baseline performance metrics on the effects of irradiation on the gut microbiome of mass-reared flies in Hawai’i, a study was performed to assess performance metrics and microbiome (bacterial 16S-rRNA) variation across multiple timepoints. Mediterranean fruit fly pupae were selected from mass-reared trays intended for release, and paired samples were either irradiated or remained as controls and transported to the laboratory for evaluation.,

,This dataset reports discovery and initial comparative analysis of 88 presumptive microRNA (miRNA) sequences from the stable fly, obtained using high-throughput sequencing of small RNAs. The majority of stable fly miRNAs were 22-23 nucleotides (nt) in length. Many miRNAs were arthropod specific, and several mature miRNA sequences showed greater sequence identity to miRNAs from other blood-feeding dipterans such as mosquitoes rather than to Drosophilids. This initial step in characterizing the stable fly microRNAome provides a basis for further analyses of life stage-specific and tissue-specific expression to elucidate their functional roles in stable fly biology.,The stable fly, Stomoxys calcitrans (L.), is a serious ectoparasite affecting animal production and health of both animals and humans. Stable fly control relies largely on chemical insecticides; however, the development of insecticide resistance as well as environmental considerations requires continued discovery research to develop novel control technologies. MicroRNAs are a class of short noncoding RNAs that have been shown to be important regulators of gene expression across a wide variety of organisms, and may provide an innovative approach with regard to development of safer more targeted control technologies.,,

,To provide a foundation for identification of genomic loci for insecticide resistance and for discovery of new control technology, we report the sequencing, assembly, and annotation of the horn fly genome.,,

,Adult house flies (Musca domestica L.) are important reservoirs and mechanical vectors of bacteria in livestock operations. House fly bacterial communities are influenced by their local environment, yet a comprehensive understanding of bacterial diversity, pathogen prevalence, and bacterial source is not fully understood. We characterized bacterial communities from adult female house flies and associated manure samples from beef and dairy cattle farms in Kansas, Oklahoma, and Texas over four months (July-October). Bacterial community composition in flies and manure reflected the local environment, and house flies shared the majority (≥99%) of bacterial taxa with manure. The variability of bacterial diversity was greater among individual fly (species richness range: 48 - 1747) samples than manure (species richness range: 345 - 1162). Temporal variability of fly bacterial diversity was observed within each farm type. Bacterial taxa of veterinary and medical importance such as Corynebacterium, Turicibacter, Staphylococcus, Streptococcus, and Acinetobacter were highly prevalent in flies, constituting core bacterial communities. The prevalence of bacterial taxa associated with bovine keratoconjunctivitis (IBK) and bovine respiratory disease (BRD) was higher in flies than in manure and prevalence varied monthly. This study underscores the crucial role house flies play as carriers of cattle pathogens, contributing to their dissemination among animals and to off-site locations, where they pose a threat to surrounding communities and agricultural operations.,The raw Illumina MiSeq sequence data for this project can be found here: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1009094,Resources in this dataset:,,

This data release includes sampling location data, the information about sequenced organisms and the SRA accession number for the 16S whole-organism sequencing data for larval aquatic insects, emergent adult insects, and two riparian spiders from Torch Lake and Gratiot Lake (N=3 sites at each Lake). Torch Lake (Houghton County, Keweenaw Peninsula, Michigan, USA) is a Great Lakes Area of Concern (AOC) and a former EPA Superfund Site and owing to a high concentration of copper and other co-occurring metals from historic mining operations, while no mining or processing activity has taken place in the nearby Gratiot Lake basin (Keweenaw County, Keweenaw Peninsula, Michigan, USA).

,Adult house flies (Musca domestica L.) inhabiting dairy farms not only are nuisance pests but also harbor and disseminate bacteria. We examined the bacterial community composition, diversity, environmental sources, and prevalence in individual adult female house flies and cattle manure samples collected monthly from Florida, North Carolina, and Tennessee dairy farms between May and August 2021. Individual house flies carried diverse bacterial communities, encompassing all bacterial taxa (100%) identified in across manure samples, and additional species likely acquired from the animals. Bacterial community assemblage in house flies and manure samples within farms varied by month. Some taxa were differentially associated with either house flies (Corynebacterium, Acinetobacter, and Staphylococcus) or manure samples (Treponema, Succinivibrio, and Clostridia). House fly bacterial communities mostly contained specialist species originating from manure, with several taxa (Escherichia, Corynebacterium, Turicibacter) being potential pathogens of livestock and humans. These findings further support the role of house flies as carriers of cattle-associated bacteria, including pathogens, and their potential for disseminating these microbes among cattle and to neighboring environments. Since their bacterial communities provide a snapshot of their surrounding environment, house flies also serve as effective sentinels in xenosurveillance strategies.,The raw Illumina MiSeq sequence data for this project can be found here: https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1070634,Resources in this dataset:,,

,Meta-data from over 100 studies that examined induction of plant phenolic compounds following colonization by insects or microbial organisms (both pathogens and beneficials). This meta-data was used in a meta-analysis to observe that both insects and microbes consistently induce phenolic production. The meta-data covers publications that range from 2008 to 2017.,,