In zebrafish, 161 compounds were screened and 34 were identified by visual inspection as VDCs, of which 28 were confirmed as VDCs by quantitative image analysis. Testing of the zebrafish VDCs for their capacity to inhibit endothelial tube formation in the murine yolk-sac-derived endothelial cell line C166 identified 22 compounds that both disrupted zebrafish vascular development and murine endothelial in vitro tubulogenesis. This dataset is associated with the following publication: McCollum, C., J. Conde Vancells, C. Hans, M. Vazquez-Chantada, N. Kleinstreuer, T. Tal , T. Knudsen, S. Shah, F. Merchant, R. Finnell, J. Gustafsson, R. Cabrera, and M. Bondesson. (Reproductive Toxicology) Identification of vascular disruptor compounds by a tiered analysis in zebrafish embryos and mouse embryonic endothelial cells. REPRODUCTIVE TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 70: 60-69, (2017).

This study evaluated two anti-angiogenic agents, 5HPP-33 and TNP-470, across the ToxCastDB HTS assay platform and anchored the results to complex in vitro functional assays: the rat aortic explant assay (AEA), rat whole embryo culture (WEC), and the zebrafish embryotoxicity (ZET) assay. This dataset is not publicly accessible because: no EPA data; all the data generated by external organizations; EPA coauthors. It can be accessed through the following means: Data generated by external organizations. Format: N/A. This dataset is associated with the following publication: Ellis-Hutchings, R., R. Settivari, A. McCoy, N. Kleinstreuer, J. Franzosa, T. Knudsen, and E. Carney. (REPRODUCTIVE TOXICOLOGY) EMBRYONIC VASCULAR DISRUPTION ADVERSE OUTCOMES: LINKING HIGH THROUGHPUT SIGNALING SIGNATURES WITH FUNCTIONAL CONSEQUENCES. REPRODUCTIVE TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 70: 82-96, (2017).

The instructions for analysis are described in Kolli et al. (2019) along with a sample Illumina sequence file available with a ready-to-run VirtualMachine that can also be accessed at http://toxicology.uga.edu/resources/dna_methylation_analysis/. Supporting data for sequencing and qPCR raw data are available at https://doi.org/10.5281/zenodo.8313356, sharing qPCR raw data files for the endpoint for male and female fish from CFX Manger (Ver. 3.0), qPCR quantification data, TGBS raw sequences, and a TGBS metadata file with sample information. This dataset is associated with the following publication: Kolli, R., T. Glenn, R. Bringolf,, W. Henderson, B. Cummings, and J. Kenneke. Changes in CpG Methylation of the Vitellogenin 1 Promoter in Adult Male Zebrafish after Exposure to 17α-Ethynylestradiol. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 43(7): 1547-1556, (2024).

The data set includes: 1) Swimbladder inflation at 120 hours post-fertilization (hpf) 2) Swimbladder surface area at 120 hpf 3) Larval body length at 120 hpf 4) Swimming activity at 120 hpf 5) Targeted whole body gene expression results for nine mRNA transcripts evaluated at 60 hpf (hb9; fgf10a; acta2; anxa5b; wnt5b; fzd2; shha; ihha; ptc1) 6) Targeted whole body gene expression results for the same nine mRNA transcripts evaluated at 120 hpf. 7) Whole body thyroid hormone concentrations measured at 48, 60, 96, and 120 hpf 8) Whole body thyroid hormone concentrations in IOP exposed embryos/larvae at 48, 60, and 120 hpf 9) Data for supplementary figures S6, S7A, S7B 10) Ratio T3 of IOP exposed embryos over control T3 levels during embryonic-juvenile development (4 replicates, Figure S9). This dataset is associated with the following publication: Van Dingenen, I., L. Vergauwen, A. Haigis, B. Blackwell, E. Stacy, D. Villeneuve, and D. Knapen. Deiodinase inhibition impairs the formation of the three posterior swim bladder tissue layers during early embryonic development in zebrafish. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 261: 106632, (2023).

This dataset includes data used to generate Figures 4C, 5B, 5C, and 5D in Tal et al. Screening for angiogenic inhibitors in zebrafish to evaluate a predictive model for developmental vascular toxicity. Reproductive Toxicology. 2017. Data underlying all other figures shown in the manuscript are included in the Supplemental Tables published with the original article. This dataset is associated with the following publication: Tal , T., C. Kilty, A. Smith, C. LaLone , B. Kennedy, A. Tennant , C. McCollum, M. Bondesson, T. Knudsen , S. Padilla , and N. Kleinstreuer. Screening for angiogenic inhibitors in zebrafish to evaluate a predictive model for developmental vascular toxicity. REPRODUCTIVE TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 70: 70-81, (2017).

The data set provides all data that were reported in results, tables, and figures associated with Stinckens et al., "The effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish". The data set includes: 1) Effects of three test chemicals on thyroperoxidase enzyme activity 2) Effects of three test chemicals on deiodinase enzyme activity 3) Thyroid hormone (T3 and T4) concentrations measured in exposed larvae 4) Measures of posterior and anterior swimbladder chamber inflation and surface areas. 5) Data on swimming behavior (distance) of exposed fish. 6) Measured concentrations of the test chemicals in the test media and tissues. This dataset is associated with the following publication: Stinckens, E., L. Vergauwen, B. Blackwell, G. Ankley, D. Villeneuve, and D. Knapen. Effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 54(10): 6213-6223, (2020).

Data describe results of a set of laboratory experiments using a previously described quantitative thiaminase I activity assay to test the thiaminase activity of whole zebrafish homogenates. Parameters described include the thiaminase activity data, dilution factors, incubation time, and tissue weight assayed. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2006.