the dataset contains NHEERL collected data on fetal male rat gestational day 18 testicular testosterone production and related data. This dataset is associated with the following publication: spade, d., C. Yue Bai , C. Lambright, J. Conley, K. Boekelheide , and E. Gray. Validation of an automated counting procedure for phthalate-induced testicular multinucleated germ cells. TOXICOLOGY LETTERS. Elsevier Science Ltd, New York, NY, USA, 290: 55-61, (2018).

the data set contains the figures and tables from the publication in addition to the means, standard errors of the mean and the sample sizes used in each group for every experiment. the data set also contains a description of the genes, their function and acronyms on the QPCR arrays used in the study. Finally, the dataset includes the histopathology reports on the uterine changes induced by the different chemicals and the criteria used by the pathologist to classify the estrogenic effects of the chemicals. This dataset is associated with the following publication: Conley, J., B. Hannas, V. Wilson, E. Gray, and J. Furr. A demonstration of the uncertainty in predicting the estrogenic activity of individual chemicals and mixtures from an in vitro estrogen receptor transcriptional activation assay (T47D-KBluc) to the in vivo uterotrophic assay using oral exposure. TOXICOLOGICAL SCIENCES. Society of Toxicology, 382-395, (2016).

Feminization of male fish and the role of endocrine-active chemicals in this phenomenon has been an area of intense study for many years. Estrone (E1), a natural steroid, is found in aquatic environments sometimes at relatively high concentrations. However, E1 has been less thoroughly studied than 17β-estradiol (E2) or 17α-ethynylestradiol due in part to a relatively lower potency in metabolically-limited estrogen receptor (ER) binding/activation assays. Recent evidence suggests that in vivo biotransformation of E1 to E2 may occur in fathead minnows (Pimephales promelas) residing in environments with high concentrations of E1, such as near wastewater treatment plants. The enzymes likely responsible for this biotransformation, 17β-hydroxysteroid dehydrogenases (17βHSDs), have been well characterized in mammals but to a lesser extent in fish species. In the current study, a novel systematic analysis of amino acid sequence data from the National Center for Biotechnology Information database demonstrated that multiple 17βHSD isoforms are conserved across different fish species. Experimentally, we showed that metabolically-active hepatic cytosolic preparations from two commercially important salmonid species, rainbow trout and lake trout, biotransformed E1 to E2 to a degree sufficient to alter results of competitive ER binding assays. These results from in silico and in vitro analyses indicate E1 and biotransformation may play a significant role in the feminization of a variety of fish species in contaminated aquatic environments. This dataset is associated with the following publication: Tapper, M., R. Kolanczyk, C. LaLone, J. Denny, and G. Ankley. Conversion of estrone to 17â-Estradiol: A potential confounding factor in assessing risks of environmental estrogens to fish. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 39(10): 2028–2040, (2020).

Adult largemouth bass have been shown to be sensitive to induction of intersex in response to chronic estrogen exposures over a complete reproductive cycle. Late summer to fall is the period of early gonad recrudescence when spermatogenesis is beginning in preparation for the spawning season in the spring. Our objective was to assess in 2018 whether early gonad recrudescence was a period of sensitivity for induction of intersex and/or alterations in reproductive condition or function. Adult male largemouth bass were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either 17alpha-ethinylestradiol or to a mixture of endocrine-active substances commonly detected in the Chesapeake Bay watershed (atrazine and estrone). Gonad samples were sent overnight to Wetland and Aquatic Research Center laboratories and sperm motility was measured by computer assisted sperm motion analysis. Overall, male reproductive condition, sperm count, and sperm motility were analyzed.

Experimental results from in vitro analysis for Confirmation of Estrogenic and Antiandrogenic Pesticide Bioactivity using Schild Regression Analysis. This dataset is associated with the following publication: Boxberger, C., L. Gray, N. Evans, J. Conley, and E. Medlock Kakaley. In Vitro Confirmation of Estrogenic and Antiandrogenic Pesticide Bioactivity using Schild Regression Analysis. TOXICOLOGY AND APPLIED PHARMACOLOGY. Elsevier B.V., Amsterdam, NETHERLANDS, 504: 117523, (2025).

Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17α-ethinylestradiol.

Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17α-ethinylestradiol.

This data set is a dose response analysis of two thyroid hormone synthesis disruptors in adult male rats. Data included serum and thyroid gland concentrations of the two chemicals tested, propylthiouracil and methimazole, as well as serum and thyroid gland hormone concentrations. These data were critical for developing a model linking highthroughput assay data on synthesis inhibition to make predictions of thyroid hormone in serum. This dataset is associated with the following publication: Hassan, I., H. El-Masri, J. Ford, A. Brennan, S. Handa, K. Friedman, and M. Gilbert. Extrapolating In Vitro and Ex Vivo Screening Assay Data for Thyroperoxidase Inhibition to Predict Serum Thyroid Hormones in the Rat. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 173(2): 280-292, (2020).

This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure study utilizing estrone and atrazine. The data for corresponding measurements of estrone in the water extracts by liquid chromatography-mass spectrometry are also shown. This data also describes relative fluorescence units of bovine serum albumin standards which were used to convert the relative fluorescence units of the cell lysates to protein with which to normalize the relative luminescence units of the cell lysates.

Three chemicals, 2-ethylhexyl 4-hydroxybenzoate (2-EHHB), 5-chloro-2-(2,4-dichlorophenoxy) phenol (triclosan), and 4-nonylphenol, branched (4-NP) were evaluated using the Larval Amphibian Growth and Development Assay (LAGDA) to investigate endocrine-mediated effects. A delay in development to NF stage 62 was observed for the three chemicals, however, histopathological evaluation revealed that the effects were likely not thyroid related. 4-NP caused increased stage scores in male and female gonadal duct development. Citation information for this dataset can be found in Data.gov's References section.