This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure study utilizing estrone and atrazine. The data for corresponding measurements of estrone in the water extracts by liquid chromatography-mass spectrometry are also shown. This data also describes relative fluorescence units of bovine serum albumin standards which were used to convert the relative fluorescence units of the cell lysates to protein with which to normalize the relative luminescence units of the cell lysates.

Compares results of bioassay with hormones measured using analytical chemistry. This dataset is associated with the following publication: Conley, J., H. Mash , N. Evans , K. Schenck , L. Rosenblum, S. Glassmeyer , E.T. Furlong, D.W. Kolpin, and V. Wilson. Comparison of in vitro estrogenic activity and estrogen concentrations in source and treated waters from 25 U.S. drinking water treatment plants. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, N/A, (2016).

In vitro bioactivity concentrations and chemical concentrations of estrogens, androgens, and glucocorticoids from a nationwide screen of United States stream water samples. In vitro bioassays include T47D-Kbluc, MDA-kb2, and a CV-1 cell line transduced with human glucocorticoid receptor. This dataset is associated with the following publication: Conley, J., N. Evans, M. Cardon, L. Rosenblum, L. Iwanowicz, P. Hartig, K. Schenck, P. Bradley, and V. Wilson. Occurrence and in vitro bioactivity of estrogen, androgen, and glucocorticoid compounds in a nationwide screen of United States stream waters. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 4781-4791, (2017).

This USGS data release contains station and laboratory method information and geospatial information, as well as concentration results for inorganic and organic compounds and bioluminescent yeast estrogen screen and transgenic zebrafish embryo estrogren bioassay, analyzed at 38 sites in 25 states as part of the Chemical Mixtures and Environmental Effects Pilot Study, 2012-2014.

This USGS data release contains station and laboratory method information and geospatial information, as well as concentration results for inorganic and organic compounds and bioluminescent yeast estrogen screen and transgenic zebrafish embryo estrogren bioassay, analyzed at 38 sites in 25 states as part of the Chemical Mixtures and Environmental Effects Pilot Study, 2012-2014.

the data set contains the figures and tables from the publication in addition to the means, standard errors of the mean and the sample sizes used in each group for every experiment. the data set also contains a description of the genes, their function and acronyms on the QPCR arrays used in the study. Finally, the dataset includes the histopathology reports on the uterine changes induced by the different chemicals and the criteria used by the pathologist to classify the estrogenic effects of the chemicals. This dataset is associated with the following publication: Conley, J., B. Hannas, V. Wilson, E. Gray, and J. Furr. A demonstration of the uncertainty in predicting the estrogenic activity of individual chemicals and mixtures from an in vitro estrogen receptor transcriptional activation assay (T47D-KBluc) to the in vivo uterotrophic assay using oral exposure. TOXICOLOGICAL SCIENCES. Society of Toxicology, 382-395, (2016).

the data set contains the figures and tables from the publication in addition to the means, standard errors of the mean and the sample sizes used in each group for every experiment. the data set also contains a description of the genes, their function and acronyms on the QPCR arrays used in the study. Finally, the dataset includes the histopathology reports on the uterine changes induced by the different chemicals and the criteria used by the pathologist to classify the estrogenic effects of the chemicals. This dataset is associated with the following publication: Conley, J., B. Hannas, V. Wilson, E. Gray, and J. Furr. A demonstration of the uncertainty in predicting the estrogenic activity of individual chemicals and mixtures from an in vitro estrogen receptor transcriptional activation assay (T47D-KBluc) to the in vivo uterotrophic assay using oral exposure. TOXICOLOGICAL SCIENCES. Society of Toxicology, 382-395, (2016).

Intersex in small- and largemouth bass has been observed in the upper Chesapeake Bay Watershed since 2003. This observation is indicative of exposure to estrogenic endocrine disrupting chemicals. In order to complement biological data sets and better identify the spatial and temporal nature of this end-point, discrete grab water samples were collected throughout the upper Chesapeake Bay Watershed for the evaluation of estrogenic activity. Data reported here are estrogenicity values derived from bioassay screening of OASIS-HLB extracts discrete grab water samples. Estrogenicity was determined using a bioluminescent yeast estrogen screen. Here we report estrogenicity data for selected sites in the Chesapeake Bay water shed collected between 2008 and 2011.

Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter), or a mixture of endocrine-active substances commonly detected in the Chesapeake Bay watershed, atrazine (5.4 micrograms per liter) and estrone (47.9 nanograms per liter). Male reproductive condition, sperm count, and sperm motility were assessed.

Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter), or a mixture of endocrine-active substances commonly detected in the Chesapeake Bay watershed, atrazine (5.4 micrograms per liter) and estrone (47.9 nanograms per liter). Male reproductive condition, sperm count, and sperm motility were assessed.