Compares results of bioassay with hormones measured using analytical chemistry. This dataset is associated with the following publication: Conley, J., H. Mash , N. Evans , K. Schenck , L. Rosenblum, S. Glassmeyer , E.T. Furlong, D.W. Kolpin, and V. Wilson. Comparison of in vitro estrogenic activity and estrogen concentrations in source and treated waters from 25 U.S. drinking water treatment plants. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, N/A, (2016).

In vitro bioactivity concentrations and chemical concentrations of estrogens, androgens, and glucocorticoids from a pilot study of US tapwaters. In vitro bioassays include T47D-Kbluc, MDA-kb2, and a CV-1 cell line transduced with human glucocorticoid receptor. This dataset is associated with the following publication: Bradley, P., D. Kolpin, K. Romanok, K. Smalling, M. Focaszio, J. Brown, M. Cardon, K. Carpenter, S. Corsi, L. DeCicco, J. Dietze, N. Evans, E. Furlong, C. Givens, J. Gray, D. Griffin, C. Higgins, M. Hladik, L. Iwanowicz, C. Journey, K. Kuivila, J. Masoner, C. McDonough, M. Meyer, J. Orlando, M. Strynar, C. Weis, and V. Wilson. Reconnaissance of Mixed Organic and Inorganic Chemicals in Private and Public Supply Tapwaters at Selected Residential and Workplace Sites in the United States. Environmental Science & Technology Letters. American Chemical Society, Washington, DC, USA, 13972-13985, (2018).

In vitro bioactivity concentrations and chemical concentrations of estrogens, androgens, and glucocorticoids from a pilot study of US tapwaters. In vitro bioassays include T47D-Kbluc, MDA-kb2, and a CV-1 cell line transduced with human glucocorticoid receptor. This dataset is associated with the following publication: Bradley, P., D. Kolpin, K. Romanok, K. Smalling, M. Focaszio, J. Brown, M. Cardon, K. Carpenter, S. Corsi, L. DeCicco, J. Dietze, N. Evans, E. Furlong, C. Givens, J. Gray, D. Griffin, C. Higgins, M. Hladik, L. Iwanowicz, C. Journey, K. Kuivila, J. Masoner, C. McDonough, M. Meyer, J. Orlando, M. Strynar, C. Weis, and V. Wilson. Reconnaissance of Mixed Organic and Inorganic Chemicals in Private and Public Supply Tapwaters at Selected Residential and Workplace Sites in the United States. Environmental Science & Technology Letters. American Chemical Society, Washington, DC, USA, 13972-13985, (2018).

This USGS data release contains station and laboratory method information and geospatial information, as well as concentration results for inorganic and organic compounds and bioluminescent yeast estrogen screen and transgenic zebrafish embryo estrogren bioassay, analyzed at 38 sites in 25 states as part of the Chemical Mixtures and Environmental Effects Pilot Study, 2012-2014.

This USGS data release contains station and laboratory method information and geospatial information, as well as concentration results for inorganic and organic compounds and bioluminescent yeast estrogen screen and transgenic zebrafish embryo estrogren bioassay, analyzed at 38 sites in 25 states as part of the Chemical Mixtures and Environmental Effects Pilot Study, 2012-2014.

This data set includes results for hormone and pharmaceutical compounds analyzed in environmental and quality-control samples collected by the USGS National Water-Quality Assessment Project during 2013 through 2015 for a study of groundwater resources used for drinking-water supply across the United States. This data release includes: Table 1. Site information and ancillary data (including principal aquifer, rock type, well depth, groundwater age information, aridity information, and land-use information) for groundwater sites sampled for hormones and pharmaceuticals by the National Water-Quality Assessment Project, 2013-15. Table 2. Hormone and pharmaceutical results for groundwater samples collected by the National Water-Quality Assessment Project, 2013-15. Table 3. Hormone and pharmaceutical results for field related blank samples of known blank type collected by the National Water-Quality Assessment Project for groundwater, 2013-15. Table 4. Hormone and pharmaceutical results for laboratory matrix spikes and associated environmental samples collected by the National Water-Quality Assessment Project for groundwater, 2013-15. Table 5. Hormone and pharmaceutical results for replicate samples and associated environmental samples collected by the National Water-Quality Assessment Project for groundwater, 2013-15.

This data set includes results for hormone and pharmaceutical compounds analyzed in environmental and quality-control samples collected by the USGS National Water-Quality Assessment Project during 2013 through 2015 for a study of groundwater resources used for drinking-water supply across the United States. This data release includes: Table 1. Site information and ancillary data (including principal aquifer, rock type, well depth, groundwater age information, aridity information, and land-use information) for groundwater sites sampled for hormones and pharmaceuticals by the National Water-Quality Assessment Project, 2013-15. Table 2. Hormone and pharmaceutical results for groundwater samples collected by the National Water-Quality Assessment Project, 2013-15. Table 3. Hormone and pharmaceutical results for field related blank samples of known blank type collected by the National Water-Quality Assessment Project for groundwater, 2013-15. Table 4. Hormone and pharmaceutical results for laboratory matrix spikes and associated environmental samples collected by the National Water-Quality Assessment Project for groundwater, 2013-15. Table 5. Hormone and pharmaceutical results for replicate samples and associated environmental samples collected by the National Water-Quality Assessment Project for groundwater, 2013-15.

Intersex in small- and largemouth bass has been observed in the upper Chesapeake Bay Watershed since 2003. This observation is indicative of exposure to estrogenic endocrine disrupting chemicals. In order to complement biological data sets and better identify the spatial and temporal nature of this end-point, discrete grab water samples were collected throughout the upper Chesapeake Bay Watershed for the evaluation of estrogenic activity. Data reported here are estrogenicity values derived from bioassay screening of OASIS-HLB extracts discrete grab water samples. Estrogenicity was determined using a bioluminescent yeast estrogen screen. Here we report estrogenicity data for selected sites in the Chesapeake Bay water shed collected between 2008 and 2011.

This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure study utilizing estrone and atrazine. The data for corresponding measurements of estrone in the water extracts by liquid chromatography-mass spectrometry are also shown. This data also describes relative fluorescence units of bovine serum albumin standards which were used to convert the relative fluorescence units of the cell lysates to protein with which to normalize the relative luminescence units of the cell lysates.

This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure study utilizing estrone and atrazine. The data for corresponding measurements of estrone in the water extracts by liquid chromatography-mass spectrometry are also shown. This data also describes relative fluorescence units of bovine serum albumin standards which were used to convert the relative fluorescence units of the cell lysates to protein with which to normalize the relative luminescence units of the cell lysates.