We supply abundance information of fish species along multiple randomly oriented transects at the lowest possible taxonomic level. This information is collected from multiple stations on an annual or biennial basis.

This is a long term data set collecting visual census transect data on reef fishes at staions located at Rileys Hump, Tortugas South Ecological Reservee.

This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrates (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources.To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions.

NCCOS' Center for Coastal Monitoring and Assessment (CCMA) is working closely with a number of divisions in the USVI DPNR (e.g., Divisions of Fish and Wildlife and Coastal Zone Management), the University of the Virgin Islands (UVI), and The Nature Conservancy (TNC) to develop the baseline characterization of chemical contamination, toxicity, and the marine resources in the St. Thomas East End Reserve (STEER) in St. Thomas, USVI. The STEER contains extensive mangroves, seagrass beds and coral reefs. Within the watershed, however, are a large active landfill, numerous marinas, various commercial/industrial activities, an EPA Superfund Site, resorts, and several residential areas served by individual septic systems. This baseline assessment will provide managers with critical information needed to help preserve and restore habitats, including a number of nursery areas within the STEER that are important to commercial and recreational fisheries. As part of the characterization, a field survey was conducted in June 2012 to conduct a biological assessment of fish communities and benthic habitats within the STEER and at select hardbottom locations adjacent to STEER. The basis for this work was the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps were stratified to select sampling stations. Sites were randomly selected within strata to ensure coverage of the entire study region. The habitat stratification was divided into three major habitat types: hardbottom which includes reef, pavement, etc. inside STEER; softbottom which consists of sand and seagrass, and mangrove. In addition, two harbottom areas outside STEER of interest to STEER's Core Team were included as a separate stratum. Using standardized protocols of NOAA's Coral Reef Ecosystem Monitoring Project, the fish and benthic habitat survey was conducted by two scientific divers. During each dive one diver quantified the species and size of fish within a 25 x 4 m transect while a second diver characterized the habitat and invertebrate community.

This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrates (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources. To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions. Starting in 2010, the regular La Parguera survey area was extended eastward to encompass the Guanica Bay region. The purpose of this modification was to conduct a baseline assessment of fish, macro-invertebrates (conch, lobster, Diadema) and benthic communities in support of Guanica Bay watershed restoration, and then to monitor changes over time. A watershed management plan was developed in 2008 by the Center for Watershed Protection, in cooperation with various Divisions of Puerto Rico DNER and NOAA, to identify priority management recommendations and implementation strategies for the Guanica watershed based on input from local experts, observations from on-the-ground assessments, and a comprehensive review of

This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrates (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources. To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions. Starting in 2010, the regular La Parguera survey area was extended eastward to encompass the Guanica Bay region. The purpose of this modification was to conduct a baseline assessment of fish, macro-invertebrates (conch, lobster, Diadema) and benthic communities in support of Guanica Bay watershed restoration, and then to monitor changes over time. A watershed management plan was developed in 2008 by the Center for Watershed Protection, in cooperation with various Divisions of Puerto Rico DNER and NOAA, to identify priority management recommendations and implementation strategies for the Guanica watershed based on input from local experts, observations from on-the-ground assessments, and a comprehensive review of

The research mission was conducted in the Dry Tortugas, FL by National Ocean Service scientists from the Center for Coastal Habitat and Fisheries Research (CCFHR) during 2009 aboard the NOAA Ship Nancy Foster. The overall objective of CCFHR's research in the Tortugas is to examine the effects of implementation of the Tortugas North Ecological Reserve (TNER). The establishment of the TNER, a no-take reserve, in 2001 provided the opportunity to examine the response of the fish and benthic communities to the creation of a refuge for exploited reef fishes. Historically, exploitation of reef fishes in the Tortugas has focused on large predatory reef fishes, primarily snappers and groupers and, to a lesser extent, grunts. Trends in populations of these targeted species are expected to vary relative to geographic variation in fishing mortality (F). Increasing trends in targeted species abundance are expected in the TNER where all fishing was prohibited in 2001. Within adjacent areas managed as the Exclusive Economic Zone (EEZ), where both federally sanctioned commercial and recreational fisheries are permitted, exploited populations are expected to be depressed relative to those in the TNER. Within the Dry Tortugas National Park (DTNP) where fishing effort is limited to recreational hook and line fishing, trends in exploited species are expected to be intermediate to those observed in the TNER and EEZ. Cascading effects due to change in abundance of exploited species is expected to indirectly impact corals and other sessile benthic communities. Sampling to detect reserve implementation effects was conducted at the ecotone between the reef habitat of the banks and the surrounding soft-bottom shelf where the structure and composition of communities should provide sensitive indicators of a reserve effect. Energy flow across reef-sand boundaries is critical to reef communities. Energy and nutrients are imported to the reef by nocturnally foraging reef fish that feed in sand, algae, and seagrass flats adjacent to the reef. The majority of the TNER (approximately 70%) consists of soft-bottom shelf habitat, and previous work on the west Florida shelf suggests that benthic primary production is the major energy source supporting fish biomass. In addition to providing ecologically sensitive sampling locations, the interface between bank and shelf provided a distinct landscape feature suited to a comparative analysis of management impact. Observations were made using 1) a stratified-random survey design for scuba divers visual observations, 2) a systematic survey of fish and fauna using scientific splitbeam echosounders (fisheries sonar) to map fish densities and biomass on the shelf, coral and softbottom habitats.

This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrate (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources.To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions.

The research mission was conducted in the Dry Tortugas, FL by National Ocean Service scientists from the Center for Coastal Habitat and Fisheries Research (CCFHR) during 2011 aboard the NOAA Ship Nancy Foster. The overall objective of CCFHR's research in the Tortugas is to examine the effects of implementation of the Tortugas North Ecological Reserve (TNER). The establishment of the TNER, a no-take reserve, in 2001 provided the opportunity to examine the response of the fish and benthic communities to the creation of a refuge for exploited reef fishes. Historically, exploitation of reef fishes in the Tortugas has focused on large predatory reef fishes, primarily snappers and groupers and, to a lesser extent, grunts. Trends in populations of these targeted species are expected to vary relative to geographic variation in fishing mortality (F). Increasing trends in targeted species abundance are expected in the TNER where all fishing was prohibited in 2001. Within adjacent areas managed as the Exclusive Economic Zone (EEZ), where both federally sanctioned commercial and recreational fisheries are permitted, exploited populations are expected to be depressed relative to those in the TNER. Within the Dry Tortugas National Park (DTNP) where fishing effort is limited to recreational hook and line fishing, trends in exploited species are expected to be intermediate to those observed in the TNER and EEZ. Cascading effects due to change in abundance of exploited species is expected to indirectly impact corals and other sessile benthic communities. Sampling to detect reserve implementation effects was conducted at the ecotone between the reef habitat of the banks and the surrounding soft-bottom shelf where the structure and composition of communities should provide sensitive indicators of a reserve effect. Energy flow across reef-sand boundaries is critical to reef communities. Energy and nutrients are imported to the reef by nocturnally foraging reef fish that feed in sand, algae, and seagrass flats adjacent to the reef. The majority of the TNER (approximately 70%) consists of soft-bottom shelf habitat, and previous work on the west Florida shelf suggests that benthic primary production is the major energy source supporting fish biomass. In addition to providing ecologically sensitive sampling locations, the interface between bank and shelf provided a distinct landscape feature suited to a comparative analysis of management impact. Observations were made using 1) a stratified-random survey design for scuba divers visual observations, 2) a systematic survey of fish and fauna using scientific splitbeam echosounders (fisheries sonar) to map fish densities and biomass on the shelf, coral and softbottom habitats.

This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrates (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources. To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions.