Genetic diversity levels, effective population size estimates, and population structure of the endangered Hawaiian hoary bat (Lasiurus semotus, also known as Aeorestes semotus) were examined across the islands of Hawai‘i, Maui, O‘ahu, and Kaua‘i using eighteen nuclear microsatellite loci and one mitochondrial gene from 339 individuals collected between 1988 and 2020. The study extracted DNA for population genetic analyses from tissue samples, collected from live bats captured as part of ongoing field studies or under rehabilitation care, from bat carcasses collected by local federal and state wildlife agencies and wind energy facilities, and from dried skin specimens at the Bernice Pauahi Bishop Museum. Eighteen nuclear microsatellite loci were amplified from 298 individuals. This dataset contains the microsatellite allele fragment sizes generated from 298 individual bat samples.

Genetic diversity levels, effective population size estimates, and population structure of the endangered Hawaiian hoary bat (Lasiurus semotus, also known as Aeorestes semotus) were examined across the islands of Hawai‘i, Maui, O‘ahu, and Kaua‘i using eighteen nuclear microsatellite loci and one mitochondrial gene from 339 individuals collected between 1988 and 2020. The study extracted DNA for population genetic analyses from tissue samples, collected from live bats captured as part of ongoing field studies or under rehabilitation care, from bat carcasses collected by local federal and state wildlife agencies and wind energy facilities, and from dried skin specimens at the Bernice Pauahi Bishop Museum. A region of the mitochondrial CO1 gene was sequenced in 321 individuals and eighteen nuclear microsatellite loci were amplified from 298 individuals. This data release contains three data files: (1) microsatellite allele fragment size data, (2) mitochondrial DNA CO1 sequences, and (3) sample collection site data and groupings used for population genetic testing.

Genetic diversity levels, effective population size estimates, and population structure of the endangered Hawaiian hoary bat (Lasiurus semotus, also known as Aeorestes semotus) were examined across the islands of Hawai‘i, Maui, O‘ahu, and Kaua‘i using eighteen nuclear microsatellite loci and one mitochondrial gene from 339 individuals collected between 1988 and 2020. The study extracted DNA for population genetic analyses from tissue samples, collected from live bats captured as part of ongoing field studies or under rehabilitation care, from bat carcasses collected by local federal and state wildlife agencies and wind energy facilities, and from dried skin specimens at the Bernice Pauahi Bishop Museum. A region of the mitochondrial CO1 gene was sequenced in 321 individuals and eighteen nuclear microsatellite loci were amplified from 298 individuals. This data release contains three data files: (1) microsatellite allele fragment size data, (2) mitochondrial DNA CO1 sequences, and (3) sample collection site data and groupings used for population genetic testing.

This dataset contains collection information for 23 Hawaiian hoary bat tissue samples collected across four Hawaiian Islands, Hawaii, Maui, Oahu, and Kauai. These data were collected in order to examine the phylogenetic history and patterns of genetic variation in the Hawaiian hoary bat. The raw sequencing data are deposited to National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) under accession PRJNA559902. The data are archived in GenBank at the following URL: https://www.ncbi.nlm.nih.gov/bioproject/559902.

This dataset contains collection information for 23 Hawaiian hoary bat tissue samples collected across four Hawaiian Islands, Hawaii, Maui, Oahu, and Kauai. These data were collected in order to examine the phylogenetic history and patterns of genetic variation in the Hawaiian hoary bat. The raw sequencing data are deposited to National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) under accession PRJNA559902. The data are archived in GenBank at the following URL: https://www.ncbi.nlm.nih.gov/bioproject/559902.

Genetic diversity levels, effective population size estimates, and population structure of the endangered Hawaiian hoary bat (Lasiurus semotus, also known as Aeorestes semotus) were examined across the islands of Hawai‘i, Maui, O‘ahu, and Kaua‘i using eighteen nuclear microsatellite loci and one mitochondrial gene from 339 individuals collected between 1988 and 2020. The study extracted DNA for population genetic analyses from tissue samples, collected from live bats captured as part of ongoing field studies or under rehabilitation care, from bat carcasses collected by local federal and state wildlife agencies and wind energy facilities, and from dried skin specimens at the Bernice Pauahi Bishop Museum. A region of the mitochondrial CO1 gene was sequenced in 321 individuals. This dataset contains the mitochondrial gene region cytochrome oxidase subunit 1 (CO1) DNA sequences generated from 321 individual bat samples.

Genetic diversity levels, effective population size estimates, and population structure of the endangered Hawaiian hoary bat (Lasiurus semotus, also known as Aeorestes semotus) were examined across the islands of Hawai‘i, Maui, O‘ahu, and Kaua‘i using eighteen nuclear microsatellite loci and one mitochondrial gene from 339 individuals collected between 1988 and 2020. The study extracted DNA for population genetic analyses from tissue samples, collected from live bats captured as part of ongoing field studies or under rehabilitation care, from bat carcasses collected by local federal and state wildlife agencies and wind energy facilities, and from dried skin specimens at the Bernice Pauahi Bishop Museum. A region of the mitochondrial CO1 gene was sequenced in 321 individuals. This dataset contains the mitochondrial gene region cytochrome oxidase subunit 1 (CO1) DNA sequences generated from 321 individual bat samples.

Hawaiian hoary bats ('ōpe'ape'a; Lasiurus semotus) were captured and tracked back to roosting locations on Hawaiʻi Island from 2018-2021. We examined resource selection at multiple spatial scales - perch location within a roost tree, roost tree, and forest stand. We used a discrete choice modeling approach to investigate roost selection and describe attributes of day-roost trees including those used as maternity roosts. ‘Ōpe‘ape‘a were found roosting in a variety of tree species and in an assortment of habitat types including native and non-native habitats. This data release consists of six tabular datasets: (1) Hawaiʻi Island ʻōpeʻapeʻa roost perch metrics, 2019–2021, (2) Hawai‘i Island ʻōpeʻapeʻa roost tree metrics, 2018–2021, (3) Hawaiʻi Island ʻōpeʻapeʻa random tree metrics, 2018-2021, (4) Hawaiʻi Island ʻōpeʻapeʻa roost stand metrics, 2018-2021, (5) Hawaiʻi Island ʻōpeʻapeʻa random stand metrics, 2018-2021, and (6) Hawaiʻi Island ʻōpeʻapeʻa roost thermal video monitoring, 2020-2021.

Hawaiian hoary bats ('ōpe'ape'a; Lasiurus semotus) were captured and tracked back to roosting locations on Hawaiʻi Island from 2018-2021. We examined resource selection at multiple spatial scales - perch location within a roost tree, roost tree, and forest stand. We used a discrete choice modeling approach to investigate roost selection and describe attributes of day-roost trees including those used as maternity roosts. ‘Ōpe‘ape‘a were found roosting in a variety of tree species and in an assortment of habitat types including native and non-native habitats. This data release consists of six tabular datasets: (1) Hawaiʻi Island ʻōpeʻapeʻa roost perch metrics, 2019–2021, (2) Hawai‘i Island ʻōpeʻapeʻa roost tree metrics, 2018–2021, (3) Hawaiʻi Island ʻōpeʻapeʻa random tree metrics, 2018-2021, (4) Hawaiʻi Island ʻōpeʻapeʻa roost stand metrics, 2018-2021, (5) Hawaiʻi Island ʻōpeʻapeʻa random stand metrics, 2018-2021, and (6) Hawaiʻi Island ʻōpeʻapeʻa roost thermal video monitoring, 2020-2021.

‘Ōpe‘ape‘a (Hawaiian hoary bats; Lasiurus semotus) were surveyed at 23 sites on Hawaiʻi Island from 33 to 2,341 m elevation from May 2018 to August 2021. Of the 23 sites, 8 were established as fixed survey sites for sampling at repeated intervals from January 2019 through January 2021. We surveyed each fixed site at least once per four-month period (January–April, May–August, September–December), with a survey comprising one to three netting events. Additional opportunistic surveys were conducted at alternate sites or on alternate dates. We captured 138 unique bats (37 female, 101 male) and recaptured 10 bats over 224 mist-netting events. Of the total 148 captured bats we affixed 131 with radio transmitters and attempted to relocate 127 (38 female; 89 male; 120 adult; 7 juvenile) bats in trees or forest stands used for day roosts on subsequent days of tracking. Of 91 bats (32 female; 59 male) relocated, males retained transmitters longer than females with a mean low estimate of 9 ± 6 days for males and 6 ± 4 days for females (time from capture to the last day a signal was confirmed). This data release consists of three tabular datasets: (1) Hawaiʻi Island ʻōpeʻapeʻa mist netting effort, 2018–2021, (2) Hawai‘i Island ʻōpeʻapeʻa captures, 2018–2021, and (3) Hawaiʻi Island ʻōpeʻapeʻa transmitter retention, 2018-2021.