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Assessment of Field and Laboratory Methods for the Detection and Analyses of Cyanobacteria and Cyanotoxins in Texas Reservoirs
In cooperation with the Texas Commission on Environmental Quality (TCEQ), the U.S. Geological Survey (USGS) utilized various field and laboratory methods to determine the presence and concentration of cyanobacteria, cyanotoxins, and taste-and-odor compounds in Texas water bodies. This data release documents the results from water-quality samples collected from 41 water bodies in Texas during 2016–19. Both qualitative and quantitative field and laboratory methods were performed. Analyses included phytoplankton taxonomy, measurements of phytoplankton biomass, and concentrations of cyanotoxins, taste-and-odor compounds, and photosynthetic pigments. Water-quality samples were also collected to provide supporting data and document existing conditions. These supporting data included dissolved solids, major ions, nutrients, and organic carbon. The study began in water year 2016 (WY16). A water year is defined as the 12-month period from October 1 through September 30 and is designated by the calendar year in which it ends. During water year 2016 and 2017, water-quality samples were analyzed for total and dissolved cyanotoxin concentrations (anatoxin, cylindrospermopsin, domoic acid, microcystin [total and 10 congeners], nodularin, okadaic acid, and saxitoxin), taste-and-odor compounds (methylisoborneol [MIB] and geosmin), chlorophyll a, pheophytin a, major ions (calcium, chloride, fluoride, magnesium, potassium, silica, sodium, and sulfate), and nutrients (nitrogen, phosphorous, and multiple species of each nutrient). In water year 2018 (WY18), analyses of cyanobacterial- and cyanotoxin gene concentrations were added to the study. In water year 2019 (WY19), the study design further expanded to include In-Situ Aqua TROLL sensors to compare the field values with the EXO2 multiparameter sonde. Each reservoir had one sampling site with the exception of three reservoirs at the beginning of WY2016. At those three reservoirs, only selected water-quality field properties were measured on site at 1-2 sampling sites —there were no water-quality samples collected at these sites. At all other sampling sites, water-quality field properties were measured every foot of the water column until the irradiance was 1 percent of the irradiance measured at the surface of the water column. Subsequently, water-quality field properties were measured every five feet to the bottom of the water column. Three rapid-assessment field kits were used to determine semi-quantitative values of three cyanotoxins (anatoxin, cylindrospermopsin, and microcystin) at each sampling site. Chlorophyll-a and pheophytin-a were analyzed by the Trinity River Authority Central Laboratory in Dallas, Texas. Cyanobacterial and cyanotoxin genes were analyzed by the USGS Ohio Water Microbiology Laboratory in Columbus, Ohio. The USGS Organic Geochemistry Research Laboratory in Lawrence, Kansas analyzed for cyanotoxins and taste-and-odor compounds. PhycoTech, Inc. determined phytoplankton taxonomy and biomass. Engineering Performance Solutions in Jacksonville, Florida analyzed for MIB and geosmin. Samples were analyzed for nutrients and major ions by the USGS National Water Quality Laboratory in Denver, Colorado. Water-quality field properties (water temperature, dissolved-oxygen concentration, pH, specific conductance, turbidity, chlorophyll density, chlorophyll fluorescence, phycocyanin density, phycocyanin fluorescence, irradiance, and Secchi depth) were also measured at each sampling site. Water-quality field properties and water-quality constituents are commonly referred to as “parameters” by analytical laboratories, and laboratory terminology for the datasets described in this data release were retained.
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Assessment of Field and Laboratory Methods for the Detection and Analyses of Cyanobacteria and Cyanotoxins in Texas Reservoirs
공공데이터포털
In cooperation with the Texas Commission on Environmental Quality (TCEQ), the U.S. Geological Survey (USGS) utilized various field and laboratory methods to determine the presence and concentration of cyanobacteria, cyanotoxins, and taste-and-odor compounds in Texas water bodies. This data release documents the results from water-quality samples collected from 41 water bodies in Texas during 2016–19. Both qualitative and quantitative field and laboratory methods were performed. Analyses included phytoplankton taxonomy, measurements of phytoplankton biomass, and concentrations of cyanotoxins, taste-and-odor compounds, and photosynthetic pigments. Water-quality samples were also collected to provide supporting data and document existing conditions. These supporting data included dissolved solids, major ions, nutrients, and organic carbon. The study began in water year 2016 (WY16). A water year is defined as the 12-month period from October 1 through September 30 and is designated by the calendar year in which it ends. During water year 2016 and 2017, water-quality samples were analyzed for total and dissolved cyanotoxin concentrations (anatoxin, cylindrospermopsin, domoic acid, microcystin [total and 10 congeners], nodularin, okadaic acid, and saxitoxin), taste-and-odor compounds (methylisoborneol [MIB] and geosmin), chlorophyll a, pheophytin a, major ions (calcium, chloride, fluoride, magnesium, potassium, silica, sodium, and sulfate), and nutrients (nitrogen, phosphorous, and multiple species of each nutrient). In water year 2018 (WY18), analyses of cyanobacterial- and cyanotoxin gene concentrations were added to the study. In water year 2019 (WY19), the study design further expanded to include In-Situ Aqua TROLL sensors to compare the field values with the EXO2 multiparameter sonde. Each reservoir had one sampling site with the exception of three reservoirs at the beginning of WY2016. At those three reservoirs, only selected water-quality field properties were measured on site at 1-2 sampling sites —there were no water-quality samples collected at these sites. At all other sampling sites, water-quality field properties were measured every foot of the water column until the irradiance was 1 percent of the irradiance measured at the surface of the water column. Subsequently, water-quality field properties were measured every five feet to the bottom of the water column. Three rapid-assessment field kits were used to determine semi-quantitative values of three cyanotoxins (anatoxin, cylindrospermopsin, and microcystin) at each sampling site. Chlorophyll-a and pheophytin-a were analyzed by the Trinity River Authority Central Laboratory in Dallas, Texas. Cyanobacterial and cyanotoxin genes were analyzed by the USGS Ohio Water Microbiology Laboratory in Columbus, Ohio. The USGS Organic Geochemistry Research Laboratory in Lawrence, Kansas analyzed for cyanotoxins and taste-and-odor compounds. PhycoTech, Inc. determined phytoplankton taxonomy and biomass. Engineering Performance Solutions in Jacksonville, Florida analyzed for MIB and geosmin. Samples were analyzed for nutrients and major ions by the USGS National Water Quality Laboratory in Denver, Colorado. Water-quality field properties (water temperature, dissolved-oxygen concentration, pH, specific conductance, turbidity, chlorophyll density, chlorophyll fluorescence, phycocyanin density, phycocyanin fluorescence, irradiance, and Secchi depth) were also measured at each sampling site. Water-quality field properties and water-quality constituents are commonly referred to as “parameters” by analytical laboratories, and laboratory terminology for the datasets described in this data release were retained.
Assessment of Field and Laboratory Methods for the Detection and Analyses of Cyanobacteria and Cyanotoxins in Texas Reservoirs
공공데이터포털
In cooperation with the Texas Commission on Environmental Quality (TCEQ), the U.S. Geological Survey (USGS) used various field and laboratory methods to determine the presence and concentration of cyanobacteria, cyanotoxins, and taste-and-odor compounds in selected Texas water bodies. This data release documents the results from water-quality samples collected from 12 water bodies in Texas during water year 2020 (WY20) and 2021 (WY21). A water year is defined as the 12-month period from October 1 through September 30 and is designated by the calendar year in which it ends. Both qualitative and quantitative field and laboratory methods were performed. Analyses included phytoplankton taxonomy, measurements of phytoplankton biomass, and concentrations of cyanotoxins, taste-and-odor compounds, and photosynthetic pigments. Water-quality samples were also collected to provide supporting data and document existing conditions. These supporting data included dissolved solids, major ions, nutrients, and organic carbon. Water-quality samples were analyzed for total cyanotoxin concentrations (anatoxin, cylindrospermopsin, domoic acid, microcystin [total and 10 congeners], nodularin, okadaic acid, and saxitoxin), taste-and-odor compound concentration (2-Methylisoborneo [MIB] and geosmin), chlorophyll a, pheophytin a, major ions (calcium, chloride, fluoride, magnesium, potassium, silica, sodium, and sulfate), and nutrients (nitrogen, phosphorous, and multiple species of each nutrient). Analyses of cyanobacterial and cyanotoxin gene concentrations are included. An In-Situ Aqua TROLL multiparameter sonde was deployed concurrently with a YSI EXO2 multiparameter sonde to provide two sets of field values that can be compared. Each reservoir had one sampling site. At each site, depth-integrated samples were collected using a peristaltic pump integrating through the photic zone. The photic zone is the depth when measured irradiance is 1 percent of the irradiance measured at the surface of the water column. Water-quality field properties were measured using the multiparameter sondes at 1-foot intervals in the water column through the photic zone (the upper layer of a water body where there is sufficient sunlight penetration to support photosynthesis), then at 5-foot intervals to the bottom of the water column. Three rapid-assessment field kits were used to determine semi-quantitative values of three cyanotoxins (anatoxin, cylindrospermopsin, and microcystin) at each sampling site. Chlorophyll-a and pheophytin-a were analyzed by the Trinity River Authority Central Laboratory in Dallas, Texas. Cyanobacterial and cyanotoxin genes were analyzed by the USGS Ohio Water Microbiology Laboratory in Columbus, Ohio. The USGS Organic Geochemistry Research Laboratory in Lawrence, Kansas analyzed for cyanotoxins and taste-and-odor compounds. PhycoTech, Inc. in St. Joseph, Michigan analyzed phytoplankton taxonomy and biomass. Taxonomic names within this data release are from PhycoTech's taxonomic naming convention and may differ from the taxonomic names listed in the Integrated Taxonomic Information System database (ITIS, 2022). Engineering Performance Solutions in Jacksonville, Florida analyzed for MIB and geosmin. Samples were analyzed for suspended solids, nutrients, and major ions by the USGS National Water Quality Laboratory (NWQL) in Denver, Colorado. Water-quality field properties (water temperature, dissolved-oxygen concentration, pH, specific conductance, turbidity, chlorophyll florescence (RFU & density), phycocyanin florescence (RFU & density), irradiance, and Secchi depth) were also measured at each sampling site. NWQL terms "parameter codes" and "parameter descriptions" were retained in the water-quality dataset when referring to water-quality field properties and constituents.
Assessment of Field and Laboratory Methods for the Detection and Analyses of Cyanobacteria and Cyanotoxins in Texas Reservoirs, 2020
공공데이터포털
In cooperation with the Texas Commission on Environmental Quality (TCEQ), the U.S. Geological Survey (USGS) used various field and laboratory methods to determine the presence and concentration of cyanobacteria, cyanotoxins, and taste-and-odor compounds in selected Texas water bodies. This data release documents the results from water-quality samples collected from 12 water bodies in Texas during water year 2020 (WY20) and 2021 (WY21). A water year is defined as the 12-month period from October 1 through September 30 and is designated by the calendar year in which it ends. Both qualitative and quantitative field and laboratory methods were performed. Analyses included phytoplankton taxonomy, measurements of phytoplankton biomass, and concentrations of cyanotoxins, taste-and-odor compounds, and photosynthetic pigments. Water-quality samples were also collected to provide supporting data and document existing conditions. These supporting data included dissolved solids, major ions, nutrients, and organic carbon. Water-quality samples were analyzed for total cyanotoxin concentrations (anatoxin, cylindrospermopsin, domoic acid, microcystin [total and 10 congeners], nodularin, okadaic acid, and saxitoxin), taste-and-odor compound concentration (2-Methylisoborneo [MIB] and geosmin), chlorophyll a, pheophytin a, major ions (calcium, chloride, fluoride, magnesium, potassium, silica, sodium, and sulfate), and nutrients (nitrogen, phosphorous, and multiple species of each nutrient). Analyses of cyanobacterial and cyanotoxin gene concentrations are included. An In-Situ Aqua TROLL multiparameter sonde was deployed concurrently with a YSI EXO2 multiparameter sonde to provide two sets of field values that can be compared. Each reservoir had one sampling site. At each site, depth-integrated samples were collected using a peristaltic pump integrating through the photic zone. The photic zone is the depth when measured irradiance is 1 percent of the irradiance measured at the surface of the water column. Water-quality field properties were measured using the multiparameter sondes at 1-foot intervals in the water column through the photic zone (the upper layer of a water body where there is sufficient sunlight penetration to support photosynthesis), then at 5-foot intervals to the bottom of the water column. Three rapid-assessment field kits were used to determine semi-quantitative values of three cyanotoxins (anatoxin, cylindrospermopsin, and microcystin) at each sampling site. Chlorophyll-a and pheophytin-a were analyzed by the Trinity River Authority Central Laboratory in Dallas, Texas. Cyanobacterial and cyanotoxin genes were analyzed by the USGS Ohio Water Microbiology Laboratory in Columbus, Ohio. The USGS Organic Geochemistry Research Laboratory in Lawrence, Kansas analyzed for cyanotoxins and taste-and-odor compounds. PhycoTech, Inc. in St. Joseph, Michigan analyzed phytoplankton taxonomy and biomass. Taxonomic names within this data release are from PhycoTech's taxonomic naming convention and may differ from the taxonomic names listed in the Integrated Taxonomic Information System database (ITIS, 2022). Engineering Performance Solutions in Jacksonville, Florida analyzed for MIB and geosmin. Samples were analyzed for suspended solids, nutrients, and major ions by the USGS National Water Quality Laboratory (NWQL) in Denver, Colorado. Water-quality field properties (water temperature, dissolved-oxygen concentration, pH, specific conductance, turbidity, chlorophyll florescence (RFU & density), phycocyanin florescence (RFU & density), irradiance, and Secchi depth) were also measured at each sampling site. NWQL terms "parameter codes" and "parameter descriptions" were retained in the water-quality dataset when referring to water-quality field properties and constituents.
Assessment of Field and Laboratory Methods for the Detection and Analyses of Cyanobacteria and Cyanotoxins in Texas Reservoirs, 2020
공공데이터포털
In cooperation with the Texas Commission on Environmental Quality (TCEQ), the U.S. Geological Survey (USGS) used various field and laboratory methods to determine the presence and concentration of cyanobacteria, cyanotoxins, and taste-and-odor compounds in selected Texas water bodies. This data release documents the results from water-quality samples collected from 12 water bodies in Texas during water year 2020 (WY20) and 2021 (WY21). A water year is defined as the 12-month period from October 1 through September 30 and is designated by the calendar year in which it ends. Both qualitative and quantitative field and laboratory methods were performed. Analyses included phytoplankton taxonomy, measurements of phytoplankton biomass, and concentrations of cyanotoxins, taste-and-odor compounds, and photosynthetic pigments. Water-quality samples were also collected to provide supporting data and document existing conditions. These supporting data included dissolved solids, major ions, nutrients, and organic carbon. Water-quality samples were analyzed for total cyanotoxin concentrations (anatoxin, cylindrospermopsin, domoic acid, microcystin [total and 10 congeners], nodularin, okadaic acid, and saxitoxin), taste-and-odor compound concentration (2-Methylisoborneo [MIB] and geosmin), chlorophyll a, pheophytin a, major ions (calcium, chloride, fluoride, magnesium, potassium, silica, sodium, and sulfate), and nutrients (nitrogen, phosphorous, and multiple species of each nutrient). Analyses of cyanobacterial and cyanotoxin gene concentrations are included. An In-Situ Aqua TROLL multiparameter sonde was deployed concurrently with a YSI EXO2 multiparameter sonde to provide two sets of field values that can be compared. Each reservoir had one sampling site. At each site, depth-integrated samples were collected using a peristaltic pump integrating through the photic zone. The photic zone is the depth when measured irradiance is 1 percent of the irradiance measured at the surface of the water column. Water-quality field properties were measured using the multiparameter sondes at 1-foot intervals in the water column through the photic zone (the upper layer of a water body where there is sufficient sunlight penetration to support photosynthesis), then at 5-foot intervals to the bottom of the water column. Three rapid-assessment field kits were used to determine semi-quantitative values of three cyanotoxins (anatoxin, cylindrospermopsin, and microcystin) at each sampling site. Chlorophyll-a and pheophytin-a were analyzed by the Trinity River Authority Central Laboratory in Dallas, Texas. Cyanobacterial and cyanotoxin genes were analyzed by the USGS Ohio Water Microbiology Laboratory in Columbus, Ohio. The USGS Organic Geochemistry Research Laboratory in Lawrence, Kansas analyzed for cyanotoxins and taste-and-odor compounds. PhycoTech, Inc. in St. Joseph, Michigan analyzed phytoplankton taxonomy and biomass. Taxonomic names within this data release are from PhycoTech's taxonomic naming convention and may differ from the taxonomic names listed in the Integrated Taxonomic Information System database (ITIS, 2022). Engineering Performance Solutions in Jacksonville, Florida analyzed for MIB and geosmin. Samples were analyzed for suspended solids, nutrients, and major ions by the USGS National Water Quality Laboratory (NWQL) in Denver, Colorado. Water-quality field properties (water temperature, dissolved-oxygen concentration, pH, specific conductance, turbidity, chlorophyll florescence (RFU & density), phycocyanin florescence (RFU & density), irradiance, and Secchi depth) were also measured at each sampling site. NWQL terms "parameter codes" and "parameter descriptions" were retained in the water-quality dataset when referring to water-quality field properties and constituents.
Cyanotoxin concentrations in extracts from cyanobacteria colonies, plankton net tows, and Solid Phase Adsorption Toxin Tracking (SPATT) samplers in western rivers, lakes, and reservoirs, including drinking water sources in the Oregon Cascades: 2016-2020
공공데이터포털
This data release contains cyanotoxin concentrations for microcystins, cylindrospermopsins, anatoxins, and saxitoxins assessed using Enzyme-Linked Immunosorbent Assays (ELISA) on 363 samples collected from 82 surface-water sites located in the Cascade Range in Oregon, and eight sites located outside of the Oregon Cascade Range in Washington and California, during 2016-2020. Three sample types were assessed: 1) benthic colonies and mats of cyanobacteria (n=109), 2) plankton net tows (n=90), and 3) Solid Phase Adsorption Toxin Tracking samplers (SPATTs)(n=164).
Cyanotoxin concentrations in extracts from cyanobacteria colonies, plankton net tows, and Solid Phase Adsorption Toxin Tracking (SPATT) samplers in western rivers, lakes, and reservoirs, including drinking water sources in the Oregon Cascades: 2016-2020
공공데이터포털
This data release contains cyanotoxin concentrations for microcystins, cylindrospermopsins, anatoxins, and saxitoxins assessed using Enzyme-Linked Immunosorbent Assays (ELISA) on 363 samples collected from 82 surface-water sites located in the Cascade Range in Oregon, and eight sites located outside of the Oregon Cascade Range in Washington and California, during 2016-2020. Three sample types were assessed: 1) benthic colonies and mats of cyanobacteria (n=109), 2) plankton net tows (n=90), and 3) Solid Phase Adsorption Toxin Tracking samplers (SPATTs)(n=164).
Cyanobacteria, other water-quality, and discharge data collected from the Raritan River Basin, New Jersey, August 2020 through August 2021
공공데이터포털
Within New Jersey’s Raritan Basin Water Supply Complex, multiple lakes and reservoirs with persistent and recurrent cyanobacterial harmful algal blooms (cyanoHABs) discharge water which ultimately travels downstream in surface water to drinking-water intakes. Cyanobacteria and other water-quality data were collected as part of a collaborative study among multiple agencies, including the U.S. Geological Survey (USGS) New Jersey Water Science Center (NJWSC), New Jersey Water Supply Authority, New Jersey Department of Environmental Protection, and Montclair State University to evaluate the spatial and temporal variability of cyanotoxin occurrence and potential production, persistence, and transport from lacustrine sources to downstream fluvial systems used as a drinking-water source in the Raritan Basin Water Supply Complex, New Jersey. An advanced monitoring strategy using a combination of solid phase adsorption toxin tracking (SPATT) passive samplers, discrete water-quality samples, and continuous monitoring instrumentation, were used to help gain insight on rapidly changing water-quality conditions that affect cyanotoxin production and transport. Eight discrete sampling locations were chosen based upon existing USGS streamflow-gaging stations as well as one site located on Spruce Run Reservoir. Twenty discrete sampling events were conducted across the 8 river sampling locations and 24 sampling events from the reservoir sampling location from August 2020 through August 2021; events occurred twice per month except from December 2020 to April 2021, when samples were collected once per month. Each sampling event was spread over two days, with upstream sites sampled on the first day and downstream sites sampled on the second day. This sampling method mimicked the natural order of streamflow. To meet study objectives, certain types of data were collected, including streamflow, discrete water quality and turbidity samples, water-quality field measurements (water temperature, pH, dissolved oxygen, and specific conductance), nutrients (ammonia, orthophosphate, nitrate plus nitrite, total phosphorus, and total nitrogen), chlorophyll-a, cyanobacterial genes (cyanobacteria 16S cyanobacterial ribosomal RNA, and cyanotoxin synthetase genes for microcystin, saxitoxin, anatoxin-a, and cylindrospermopsin), cyanotoxins (microcystin, anatoxin-a, and cylindrospermopsin), and phytoplankton.
Liquid Chromatography Triple Quadrupole Mass Spectrometry (LC/MS/MS) analysis of cyanobacteria cultures from Lake Elsinore and Canyon Lake (CA, USA, 2016) for cyanotoxins and algal toxins
공공데이터포털
Cyanobacteria are common in inland water bodies. Many strains are known to produce potent toxins (cyanotoxins) which can impact human and animal health in sufficient concentrations. Lake Elsinore and Canyon Lake are two impaired lakes in California with frequent cyanobacteria blooms that are not monitored for toxin production. These data document the liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) results for 21 cyanotoxins and algal toxins in 13 cyanobacteria samples collected during bloom events from Lake Elsinore and Canyon Lake (CA, USA) in July, August, and September 2016.
Liquid Chromatography Triple Quadrupole Mass Spectrometry (LC/MS/MS) analysis of cyanobacteria cultures from Lake Elsinore and Canyon Lake (CA, USA, 2016) for cyanotoxins and algal toxins
공공데이터포털
Cyanobacteria are common in inland water bodies. Many strains are known to produce potent toxins (cyanotoxins) which can impact human and animal health in sufficient concentrations. Lake Elsinore and Canyon Lake are two impaired lakes in California with frequent cyanobacteria blooms that are not monitored for toxin production. These data document the liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) results for 21 cyanotoxins and algal toxins in 13 cyanobacteria samples collected during bloom events from Lake Elsinore and Canyon Lake (CA, USA) in July, August, and September 2016.
Cyanotoxins in the California Sacramento-San Joaquin Delta: Whole Water Cyanotoxin Samples
공공데이터포털
This dataset is comprised of cyanotoxin concentrations from whole water cyanotoxin samples collected at water quality stations in the Sacramento-San Joaquin Delta, beginning in 2020. Cyanotoxin congeners were measured from whole water cyanotoxin samples within the cyanotoxin classes: microcystins, anatoxins, cylindrospermopsins, saxitoxins, anabaenopeptins, and nodularin. Concentrations were measured using liquid chromatography tandem mass spectrometry (LC-MS/MS) and enzyme linked immunosorbent assay (ELISA).