Laboratory analysis of innate American kestrel, Falco sparverius, immune response after exposure to flame retardant, isopropylphenyl phosphate (ITP) through 21 days post hatch. Data consist of flow cytometry files that were generated in the analysis of white blood cells from kestrel blood. Thus, data are in standard format that allows files created by one type of acquisition hardware and software to be analyzed by any other type.

Laboratory analysis of innate American kestrel, Falco sparverius, immune response after exposure to flame retardant, isopropylphenyl phosphate (ITP) through 21 days post hatch. Data consist of flow cytometry files that were generated in the analysis of white blood cells from kestrel blood. Thus, data are in standard format that allows files created by one type of acquisition hardware and software to be analyzed by any other type.

This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, spleens and bursas from American kestrels (Falco sparverius) exposed by egg injection to varying doses of short-chain chlorinated paraffins (SCCPs) and the flame retardant TBBPA-BDBPE were examined microscopically for architectural and cellular abnormalities. At euthanasia, spleen and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative and qualitative B and T cell parameters were assessed by light microscopy. Specifically, variables assessed included the following: spleen: total area; number, thickness and area of peri-arteriolar lymphoid sheaths; number and diameter of lymphoid follicles; bursa: follicular and medullary area; cellular density; apoptosis; heterophil infiltration; presence of follicular cysts. Evaluation of the architecture and cellular population of immune organs will shed light on potential functional immunological effects of exposure that may lead to increased susceptibility to infectious disease or affect normal growth and development of the chick. (Luna LG. 1968. Manual of histologic staining methods of the armed forces institute of pathology, 3rd edn. McGraw-Hill, New York, NY.)

This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, spleens and bursas from American kestrels (Falco sparverius) exposed by egg injection to varying doses of short-chain chlorinated paraffins (SCCPs) and the flame retardant TBBPA-BDBPE were examined microscopically for architectural and cellular abnormalities. At euthanasia, spleen and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative and qualitative B and T cell parameters were assessed by light microscopy. Specifically, variables assessed included the following: spleen: total area; number, thickness and area of peri-arteriolar lymphoid sheaths; number and diameter of lymphoid follicles; bursa: follicular and medullary area; cellular density; apoptosis; heterophil infiltration; presence of follicular cysts. Evaluation of the architecture and cellular population of immune organs will shed light on potential functional immunological effects of exposure that may lead to increased susceptibility to infectious disease or affect normal growth and development of the chick. (Luna LG. 1968. Manual of histologic staining methods of the armed forces institute of pathology, 3rd edn. McGraw-Hill, New York, NY.)

This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, hatchling American kestrels (Falco sparverius) were exposed to the flame retardant isopropyl triphenyl phosphate (ITP) and then challenged with a synthetic analogue of viral double-stranded RNA, polyinosinic:polycytidylic acid (poly I:C). Control birds were challenged with vehicle only or vehicle and poly I:C. At euthanasia, spleen, thymus, and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative and qualitative B and T cell parameters were assessed by light microscopy. Specifically, variables assessed included the following: spleen: proportion of white to red pulp; thickness of peri-arteriolar lymphoid sheaths; number and diameter of lymphoid follicles; thymus: total area; area of medulla; density of cortical lymphocytes; number of tingible body macrophages; heterophil infiltration; bursa: follicular and medullary area; cellular density; apoptosis; heterophil infiltration; presence of follicular cysts. Evaluation of the architecture and cellular population of immune organs will shed light on potential functional immunological effects of exposure that may lead to increased susceptibility to infectious disease. (Luna LG. 1968. Manual of histologic staining methods of the armed forces institute of pathology, 3rd edn. McGraw-Hill, New York, NY.)

This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, hatchling American kestrels (Falco sparverius) were exposed to the flame retardant isopropyl triphenyl phosphate (ITP) and then challenged with a synthetic analogue of viral double-stranded RNA, polyinosinic:polycytidylic acid (poly I:C). Control birds were challenged with vehicle only or vehicle and poly I:C. At euthanasia, spleen, thymus, and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative and qualitative B and T cell parameters were assessed by light microscopy. Specifically, variables assessed included the following: spleen: proportion of white to red pulp; thickness of peri-arteriolar lymphoid sheaths; number and diameter of lymphoid follicles; thymus: total area; area of medulla; density of cortical lymphocytes; number of tingible body macrophages; heterophil infiltration; bursa: follicular and medullary area; cellular density; apoptosis; heterophil infiltration; presence of follicular cysts. Evaluation of the architecture and cellular population of immune organs will shed light on potential functional immunological effects of exposure that may lead to increased susceptibility to infectious disease. (Luna LG. 1968. Manual of histologic staining methods of the armed forces institute of pathology, 3rd edn. McGraw-Hill, New York, NY.)

This dataset describes histopathological changes in liver, kidney, heart, skeletal muscle and intestine of captive American kestrels exposed to the second-generation anticoagulant rodenticide brodifacoum (BROD). The goal of the study was to determine the toxic range of brodifacoum by feeding birds a diet containing 0.3, 1.0, or 3.0 ug BROD/g wet weight. Birds were necropsied and examined grossly for hemorrhages or anemia, and liver, kidney, heart, pectoral muscle, and intestine was collected for histopathological evaluation. Tissues were scanned at least 100x magnification and all lesions, including hemorrhage, inflammation, and degenerative changes, were described and assigned a morphologic diagnosis with severity, chronicity, and distribution as appropriate.

This dataset describes histopathological changes in liver, kidney, heart, skeletal muscle and intestine of captive American kestrels exposed to the second-generation anticoagulant rodenticide brodifacoum (BROD). The goal of the study was to determine the toxic range of brodifacoum by feeding birds a diet containing 0.3, 1.0, or 3.0 ug BROD/g wet weight. Birds were necropsied and examined grossly for hemorrhages or anemia, and liver, kidney, heart, pectoral muscle, and intestine was collected for histopathological evaluation. Tissues were scanned at least 100x magnification and all lesions, including hemorrhage, inflammation, and degenerative changes, were described and assigned a morphologic diagnosis with severity, chronicity, and distribution as appropriate.

This dataset contains results of genetic screening for Poecivirus from samples of black-capped chickadees (BCCH; Poecile atricapillus) with and without clinical signs of avian keratin disorder (AKD). Data include information on detection/non-detection of the virus in tissue collected with buccal swabs, cloacal swabs, blood samples, and fecal samples from up to 124 individuals between 2015 and 2017 from various locations in southcentral Alaska. For an additional 17 symptomatic (and one asymptomatic) individual(s) collected between 2001 and 2015 in the same region, data include measurements of viral load in beak tissue measured by qRT-PCR as well as the amount of actively replicating virus detected by 7 negative-strand and 2 positive-strand oligonucleotide probes, all relative to amounts of host RNA. Ancillary data on beak measurements, clinical signs of AKD (beak overgrowth or hyperkeratosis at the cellular level), locations, and dates of collection are also included for each individual.

- Observations of test subjects and hatching data - Body weight, organ/tissue weights - Biomarker data (oxidative stress indicators, oxidative DNA damage, thyroid hormones, histological findings) in various tissues - Chemical residues in tissues